Recombinant yeast host cells comprising FE-S cluster proteins

ABSTRACT

Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is related to and claims the benefit of priority toU.S. Provisional Application Nos. 61/100,801 filed Sep. 29, 2008 and61/100,806 filed Sep. 29, 2008. The entirety of each is herebyincorporated by reference.

FIELD OF THE INVENTION

The invention relates to the field of industrial microbiology and theexpression of proteins that require an iron-sulfur cluster for activity.More specifically, expression of heterologous Fe—S protein activity inyeast cells is improved through specific host gene inactivation.

BACKGROUND OF THE INVENTION

Engineering of yeast for fermentative production of commercial productsis an active and growing field. Enzymatic pathways engineered forbiosynthesis of some products include enzymes that require binding of aniron-sulfur (Fe—S) cluster for activity. Dihydroxy-acid dehydratase(DHAD) is one example. DHAD is part of naturally occurring biosyntheticpathways producing valine, isoleucine, leucine and pantothenic acid(vitamin B5). Increased expression of DHAD activity is desired forenhanced microbial production of branched chain amino acids or ofpantothenic acid. In addition, DHAD catalyzed conversion of2,3-dihydroxyisovalerate to α-ketoisovalerate is a common step in themultiple isobutanol biosynthetic pathways that are disclosed inco-pending US Patent Pub No. US 20070092957 A1. Disclosed therein isengineering of recombinant microorganisms for production of isobutanol,which is useful as a fuel additive and whose availability may reduce thedemand for petrochemical fuels.

Diol dehydratase provides an enzyme activity in a biosynthetic pathwayfor production of 2-butanone and 2-butanol that is disclosed inco-pending US Patent Pub No. US 2007-0292927A1. Disclosed in US PatentPub No. US20090155870 is a butanediol dehydratase that is useful forexpression in this pathway due to its coenzyme B-12 independence. A dioldehydratase reactivase that is an Fe—S cluster protein required foractivity of the B12-independent butanediol dehydratase, is alsodisclosed in US Patent Pub No. US20090155870. 2-Butanone, also referredto as methyl ethyl ketone (MEK), is a widely used solvent, extractantand activator of oxidative reactions, as well as a substrate forchemical synthesis of 2-butanol. 2-butanol is useful as a fuel additive,whose availability may reduce the demand for petrochemical fuels.

For improved production of compounds synthesized in pathways includingan Fe—S cluster containing enzyme, it is desirable to provide a hostcell capable of expressing high levels of this enzymatic activity in theproduction host of interest. Whereas a number of commercially relevantbacteria and yeast can express activity of Fe—S cluster containingproteins, this activity is at levels far below what is commerciallyuseful for enhancing introduced biosynthetic pathways. Consequently aneed exists for the discovery of host cells capable of expressingactivity of Fe—S cluster containing proteins at levels high enough toenhance introduced pathways that have Fe—S requirements. Obtaining highfunctional expression of heterologous Fe—S cluster containing enzymes isproblematic due to the Fe—S cluster requirement, which involvesavailability and proper loading of the cluster into the apo-protein.

SUMMARY OF THE INVENTION

Provided herein are recombinant yeast host cells comprising at least oneheterologous Fe—S cluster protein wherein the yeast host has reducedexpression of at least one endogenous Fe—S cluster protein.

The recombinant yeast cell may be grown under suitable conditions forthe production of products including isobutanol, 2-butanol and2-butanone.

In one aspect, the recombinant yeast cell comprises a disruption in thegene encoding the at least one endogenous Fe—S cluster protein.

In another aspect, the endogenous Fe—S cluster protein is selected fromthe group consisting of dihydroxy-acid dehydratase, isopropylmalatedehydratase, sulfite reductase, glutamate dehydrogenase, biotinsynthase, aconitase, homoaconitase, lipoate synthase, ferredoxinmaturation, NADH ubiquinone oxidoreductase, succinate dehydrogenase,ubiquinol-cytochrome-c reductase, ABC protein Rli1, NTPase Nbp35, andhydrogenase-like protein.

In another aspect, the yeast is selected from the group consisting ofSaccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces,Yarrowia and Pichia.

In another aspect, the endogenous Fe—S protein is expressed in themitochondria, and in another embodiment, the endogenous Fe—S clusterprotein has an activity selected from the group consisting of:dihydroxy-acid dehydratase and isopropylmalate dehydratase activity.

In another aspect, the host cell is Saccharomyces expressing a geneencoding a polypeptide having the amino acid sequence as set forth inSEQ ID NO:114.

In some embodiments, the at least one heterologous Fe—S cluster proteinis selected from the group consisting of fungal 2Fe-2S dihydroxy-aciddehydratases and plant 2Fe-2S dihydroxy-acid dehydratases. In oneembodiment, the heterologous fungal or plant 2Fe-2S clusterdihydroxy-acid dehydratase is expressed in the cytosol. In oneembodiment, the heterologous fungal or plant 2Fe-2S clusterdihydroxy-acid dehydratase is a polypeptide having an amino acidsequence that matches the Profile HMM of table 9 with an E value of<10⁻⁵ wherein the polypeptide additionally comprises all three conservedcysteines, corresponding to positions 56, 129, and 201 in the aminoacids sequences of the Streptococcus mutans DHAD enzyme corresponding toSEQ ID NO:179. In one embodiment, the heterologous fungal or plant2Fe-2S cluster dihydroxy-acid dehydratase is a polypeptide having anamino acid sequence that has at least about 95% sequence identity to anamino acid sequence selected from the group consisting of SEQ ID NOs:46,48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82,84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114,116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142,144, 146, 148, 150 and 152. In one embodiment, the heterologous fungalor plant 2Fe-2S cluster dihydroxy-acid dehydratase is a polypeptidehaving an amino acid sequence that is at least about 90% identical toSEQ ID NO:114 using the Clustal W method of alignment using the defaultparameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250series of protein weight matrix over the full length of the proteinsequence.

In another aspect, a method for the conversion of2,3-dihydroxyisovalerate to α-ketoisovalerate is provided, said methodcomprising:

a) providing (1) a recombinant yeast host cell comprising at least oneheterologous gene encoding a 2Fe-2S dihydroxy-acid dehydratase whereinthe recombinant yeast host cell has reduced activity of at least oneendogenous Fe—S cluster protein; and (2) a source of2,3-dihydroxyisovalerate; and

b) growing the recombinant host cell of (a) with said source of2,3-dihydroxyisovalerate under conditions where the2,3-dihydroxyisovalerate is converted by the host cell toα-ketoisovalerate.

In another aspect, a method for the conversion of 2,3-butanediol to2-butanone is provided, said method comprising:

a) providing (1) a recombinant yeast host cell comprising at least oneheterologous gene encoding a Fe—S propanediol dehydratase reactivasewherein the recombinant yeast host cell has reduced activity of at leastone endogenous Fe—S cluster protein; and (2) a source of 2,3-butanediol;and

b) growing the recombinant host cell of (a) with said source of2,3-butanediol under conditions where 2,3-butanediol is converted by thehots cell to 2-butanone.

Also provided is a method for the production of isobutanol comprisinggrowing a recombinant yeast host cell disclosed herein under conditionswherein isobutanol is produced.

In other embodiments, the at least one heterologous Fe—S cluster proteinhas Fe—S propanediol dehydratase reactivase activity. In someembodiments, the at least one heterologous Fe—S cluster protein havingFe—S propanediol dehydratase reactivase activity is a propanedioldehydratase reactivase having an amino acid sequence that is at leastabout 90% identical to the amino acid sequence as set forth in SEQ IDNO:44 using the Clustal W method of alignment using the defaultparameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250series of protein weight matrix over the full length of the proteinsequence.

In some embodiments, the cell produces 2-butanol, and in someembodiments the cell produces 2-butanone. In some embodiments, the cellcomprises a 2-butanol biosynthetic pathway, and in some embodiments, thecell comprises a 2-butanone biosynthetic pathway.

BRIEF DESCRIPTION OF THE FIGURES AND SEQUENCE DESCRIPTIONS

The invention can be more fully understood from the following detaileddescription, figures, and the accompanying sequence descriptions, whichform a part of this application.

FIG. 1 shows biosynthetic pathways for isobutanol production.

FIG. 2 shows a biosynthetic pathway for 2-butanone and 2-butanolproduction.

Table 9 is a table of the Profile HMM for dihydroxy-acid dehydratasesbased on enzymes with assayed function prepared as described inExample 1. Table 9 is submitted herewith electronically and isincorporated herein by reference.

The following sequences conform with 37 C.F.R. 1.821-1.825(“Requirements for Patent Applications Containing Nucleotide Sequencesand/or Amino Acid Sequence Disclosures—the Sequence Rules”) and areconsistent with World Intellectual Property Organization (WIPO) StandardST. 25 (1998) and the sequence listing requirements of the EPO and PCT(Rules 5.2 and 49.5(a-bis), and Section 208 and Annex C of theAdministrative Instructions). The symbols and format used for nucleotideand amino acid sequence data comply with the rules set forth in 37C.F.R. §1.822.

TABLE 1 Inactivation target Fe—S protein encoding genes SEQ ID NO: SEQID NO: Organism and gene Nucleic Acid Peptide Saccharomyces cerevisiaeLEU1 1 2 Schizosaccharomyces pombe LEU1 3 4 Candida galbrata CBS 138LEU1 5 6 Candida albicans SC 5314 LEU1 7 8 Kluyveromyces lactis LEU1 910 Yarrowia lipolytica LEU1 11 12 Pichia stipitis LEU1 13 14Saccharomyces cerevisiae YJM789 ILV3 111 112 Schizosaccharomyces pombeILV3 93 94 Candida galbrata CBS 138 ILV3 107 108 Candida albicans SC5314ILV3 101 102 Kluyveromyces lactis ILV3 113 114 Yarrowia lipolytica ILV3105 106 Pichia stipitis CBS 6054 ILV3 103 104 Saccharomyces cerevisiaeACO1 153 154 Schizosaccharomyces pombe 155 156 (chromosome II) ACO1Schizosaccharomyces pombe 157 158 (chromosome I) ACO1 Kluyveromyceslactis NRRL Y-1140 ACO1 159 160 Candida albicans SC5314 ACO1 161 162Yarrowia lipolytica CLIB122 ACO1 163 164 Pichia stipitis CBS 6054 ACO1165 166 Candida glabrata CBS138 167 168 (chromosome F) ACO1 Candidaglabrata CBS138 169 170 (chromosome D) ACO1 Candida glabrata CBS138 171172 (chromosome K) ACO1

TABLE 2 Fungal and plant 2Fe—2S DHADs in addition to those in Table 1SEQ ID NO: SEQ ID NO: Description Nucleic acid Peptide Chlamydomonasreinhardtii 45 46 Ostreococcus lucimarinus CCE9901 47 48 Vitis vinifera(Unnamed protein 49 50 product: CAO71581.1) Vitis vinifera (CAN67446.1)51 52 Arabidopsis thaliana 53 54 Oryza sativa (indica cultivar-group) 5556 Physcomitrella patens subsp. patens 57 58 Chaetomium globosum CBS148.51 59 60 Neurospora crassa OR74A 61 62 Magnaporthe grisea 70-15 6364 Gibberella zeae PH-1 65 66 Aspergillus niger 67 68 Neosartoryafischeri NRRL 181 69 70 (XP_001266525.1) Neosartorya fischeri NRRL 18171 72 (XP_001262996.1) Aspergillus niger (An03g04520) 73 74 Aspergillusniger (An14g03280) 75 76 Aspergillus terreus NIH2624 77 78 Aspergillusclavatus NRRL 1 79 80 Aspergillus nidulans FGSC A4 81 82 Aspergillusoryzae 83 84 Ajellomyces capsulatus NAm1 85 86 Coccidioides immitis RS87 88 Botryotinia fuckeliana B05.10 89 90 Phaeosphaeria nodorum SN15 9192 Pichia guilliermondii ATCC 6260 95 96 Debaryomyces hansenii CBS767 9798 Lodderomyces elongisporus NRRL 99 100 YB-4239 Vanderwaltozymapolyspora DSM 109 110 70294 Ashbya gossypii ATCC 10895 115 116 Laccariabicolor S238N-H82 117 118 Coprinopsis cinerea okayama7#130 119 120Cryptococcus neoformans var. 121 122 neoformans JEC21 Ustilago maydis521 123 124 Malassezia globosa CBS 7966 125 126 Aspergillus clavatusNRRL 1 127 128 Neosartorya fischeri NRRL 181 129 130 (Putative)Aspergillus oryzae 131 132 Aspergillus niger (An18g04160) 133 134Aspergillus terreus NIH2624 135 136 Coccidioides immitis RS (CIMG_04591)137 138 Paracoccidioides brasiliensis 139 140 Phaeosphaeria nodorum SN15141 142 Gibberella zeae PH-1 143 144 Neurospora crassa OR74A 145 146Coprinopsis cinerea okayama 7#130 147 148 Laccaria bicolor S238N-H82 149150 Ustilago maydis 521 151 152

TABLE 3 Expression genes SEQ ID NO: SEQ ID NO: Description Nucleic acidPeptide Roseburia inulinivorans (RdhtA) 15 43 Roseburia inulinivorans(RdhtB) 16 44 Bacillus subtilis (alsS) 27 28 Vibrio cholerae (KARI) 3536 Pseudomonas aeruginosa PAO1 (KARI) 37 38 Pseudomonas fluorescens PF5(KARI) 39 40 Achromobacter xylosoxidans (sadB) 41 42 B12-independentglycerol dehydratase 190 191 from Clostridium butyricum B-12 independentbutanediol 192 193 dehydratase reactivase from Clostridium butyricum

SEQ ID NO:17 is a synthetic rdhtAB sequence.

SEQ ID NOs:18-21 and 30-33 are primers for PCR, cloning or sequencinganalysis used a described in the Examples herein.

SEQ ID NO:22 is a dual terminator sequence.

SEQ ID NO:23 is the Saccharomyces cerevisiae ADH terminator.

SEQ ID NO:24 is the Saccharomyces cerevisiae CYC1 terminator.

SEQ ID NO:25 is the Saccharomyces cerevisiae FBA promoter.

SEQ ID NO:26 is the Saccharomyces cerevisiae GPM promoter.

SEQ ID NO:29 is the pNY13 vector.

SEQ ID NO:34 is the Saccharomyces cerevisiae CUP1 promoter.

SEQ ID NO:173 is the codon optimized coding region for ILV3 DHAD fromKluyveromyces lactis.

TABLE 4 Functionally verified DHADs used for Profile HMM SEQ ID NO: SEQID NO: Organism Nucleic acid Peptide Nitrosomonas europaea ATCC 19718174 175 Synechocystis sp. PCC 6803 176 177 Streptococcus mutans UA159178 179 Streptococcus thermophilus LMG 18311 180 181 Ralstoniametallidurans CH34 182 183 Ralstonia eutropha JMP134 184 185 Lactococcuslactis subsp. cremoris 186 187 SK11 Flavobacterium johnsoniae UW101 188189

DETAILED DESCRIPTION OF THE INVENTION

Disclosed herein is the discovery that introduced Fe—S containingproteins in yeast host cells have high activity levels when expressionof endogenous Fe—S containing proteins is inhibited or disrupted. Thepresent invention relates to recombinant yeast cells engineered toprovide expression of at least one heterologous protein that is an Fe—Scluster protein, and engineered for reduced expression of at least oneendogenous Fe—S cluster protein. In these cells the activity of theheterologous Fe—S cluster protein is improved, such that there isimproved production of a product made in a biosynthetic pathway thatincludes the enzyme activity. Examples of commercially useful productsfrom a pathway including an Fe—S protein include valine, isoleucine,leucine, pantothenic acid, isobutanol, 2-butanone and 2-butanol.

The following abbreviations and definitions will be used for theinterpretation of the specification and the claims.

As used herein, the terms “comprises,” “comprising,” “includes,”“including,” “has,” “having,” “contains” or “containing,” or any othervariation thereof, are intended to cover a non-exclusive inclusion. Forexample, a composition, a mixture, process, method, article, orapparatus that comprises a list of elements is not necessarily limitedto only those elements but may include other elements not expresslylisted or inherent to such composition, mixture, process, method,article, or apparatus. Further, unless expressly stated to the contrary,“or” refers to an inclusive or and not to an exclusive or. For example,a condition A or B is satisfied by any one of the following: A is true(or present) and B is false (or not present), A is false (or notpresent) and B is true (or present), and both A and B are true (orpresent).

Also, the indefinite articles “a” and “an” preceding an element orcomponent of the invention are intended to be nonrestrictive regardingthe number of instances (i.e. occurrences) of the element or component.Therefore “a” or “an” should be read to include one or at least one, andthe singular word form of the element or component also includes theplural unless the number is obviously meant to be singular.

The term “invention” or “present invention” as used herein is anon-limiting term and is not intended to refer to any single embodimentof the particular invention but encompasses all possible embodiments asdescribed in the specification and the claims.

As used herein, the term “about” modifying the quantity of an ingredientor reactant of the invention employed refers to variation in thenumerical quantity that can occur, for example, through typicalmeasuring and liquid handling procedures used for making concentrates oruse solutions in the real world; through inadvertent error in theseprocedures; through differences in the manufacture, source, or purity ofthe ingredients employed to make the compositions or carry out themethods; and the like. The term “about” also encompasses amounts thatdiffer due to different equilibrium conditions for a compositionresulting from a particular initial mixture. Whether or not modified bythe term “about”, the claims include equivalents to the quantities. Inone embodiment, the term “about” means within 10% of the reportednumerical value, preferably within 5% of the reported numerical value

The term “Fe—S cluster protein” is a protein that binds an iron-sulfurcluster and requires the binding of the cluster for its activity.

The term “2Fe-2S DHAD” refers to DHAD enzymes requiring a bound[2Fe-2S]²⁺ cluster for activity.

The term “Fe—S propanediol dehydratase reactivase” refers to propanedioldehydratase reactivases requiring a bound Fe—S cluster for activity.

The term “isobutanol biosynthetic pathway” refers to an enzyme pathwayto produce isobutanol from pyruvate.

The term “2-butanol biosynthetic pathway” refers to an enzyme pathway toproduce 2-butanol from pyruvate.

The term “2-butanone biosynthetic pathway” refers to an enzyme pathwayto produce 2-butanone from pyruvate.

There term “Dihydroxy-acid dehydratase”, also abbreviated DHAD, willrefer to an enzyme that converts 2,3-dihydroxyisovalerate toα-ketoisovalerate.

The term “butanediol dehydratase”, also known as “diol dehydratase” or“propanediol dehydratase” refers to a polypeptide (or polypeptides)having an enzyme activity that catalyzes the conversion of2,3-butanediol to 2-butanone. Butanediol dehydratases that do notutilize the cofactor adenosyl cobalamin (also known as coenzyme B12, orvitamin B12; although vitamin B12 may refer also to other forms ofcobalamin that are not coenzyme B12) are coenzyme B12-independent dioldehydratases that require association with a diol dehydratase reactivasethat is a Fe—S cluster protein. Examples of B12-independent dioldehydratases include those from Clostridium glycolicum (Hartmanis et al.(1986) Arch. Biochem. Biophys. 245:144-152), Clostridium butyricum(protein SEQ ID NO:191; coding region SEQ ID NO:190; O'Brien et al.(2004) Biochemistry 43:4635-4645), and Roseburia inulinivorans (coding:SEQ ID NO:15; protein: SEQ ID NO:43; disclosed in co-pending US PatentPub No. US20090155870.

The term “propanediol dehydratase reactivase”, also known as “dioldehydratase reactivase” or “butanediol dehydratase reactivase” refers toa reactivating factor for diol dehydratase, an enzyme which undergoessuicide inactivation during catalysis. Diol dehydratase reactivasesassociated with coenzyme B12-independent diol dehydratases may be Fe—Scluster proteins. Examples include those from Clostridium glycolicum(Hartmanis et al. (1986) Arch. Biochem. Biophys. 245:144-152),Clostridium butyricum (protein SEQ ID NO:193; coding region SEQ IDNO:192; O'Brien et al. (2004) Biochemistry 43:4635-4645), and Roseburiainulinivorans (coding: SEQ ID NO:16; protein: SEQ ID NO:44; disclosed incommonly owned and co-pending US Patent Pub No. US20090155870).

The term “reduced expression” as it applies to the expression of aprotein in a cell host will include those situations where the activityof the protein is diminished as compared with a wildtype form (as withantisense technology for example) or substantially eliminated as withgene disruption, deletion or inactivation for example.

The term “carbon substrate” or “fermentable carbon substrate” refers toa carbon source capable of being metabolized by host organisms of thepresent invention and particularly carbon sources selected from thegroup consisting of monosaccharides, oligosaccharides, polysaccharides,and one-carbon substrates or mixtures thereof.

The term “gene” refers to a nucleic acid fragment that is capable ofbeing expressed as a specific protein, optionally including regulatorysequences preceding (5′ non-coding sequences) and following (3′non-coding sequences) the coding sequence. “Native gene” refers to agene as found in nature with its own regulatory sequences. “Chimericgene” refers to any gene that is not a native gene, comprisingregulatory and coding sequences that are not found together in nature.Accordingly, a chimeric gene may comprise regulatory sequences andcoding sequences that are derived from different sources, or regulatorysequences and coding sequences derived from the same source, butarranged in a manner different than that found in nature. “Endogenousgene” refers to a native gene in its natural location in the genome ofan organism. A “foreign gene” or “heterologous gene” refers to a genenot normally found in the host organism, but that is introduced into thehost organism by gene transfer. “Heterologous gene” includes a nativecoding region, or portion thereof, that is reintroduced into the sourceorganism in a form that is different from the corresponding native gene.For example, a heterologous gene may include a native coding region thatis a portion of a chimeric gene including non-native regulatory regionsthat is reintroduced into the native host. Also a foreign gene cancomprise native genes inserted into a non-native organism, or chimericgenes. A “transgene” is a gene that has been introduced into the genomeby a transformation procedure.

As used herein the term “coding region” refers to a DNA sequence thatcodes for a specific amino acid sequence. “Suitable regulatorysequences” refer to nucleotide sequences located upstream (5′ non-codingsequences), within, or downstream (3′ non-coding sequences) of a codingsequence, and which influence the transcription, RNA processing orstability, or translation of the associated coding sequence. Regulatorysequences may include promoters, translation leader sequences, introns,polyadenylation recognition sequences, RNA processing site, effectorbinding site and stem-loop structure.

The term “promoter” refers to a DNA sequence capable of controlling theexpression of a coding sequence or functional RNA. In general, a codingsequence is located 3′ to a promoter sequence. Promoters may be derivedin their entirety from a native gene, or be composed of differentelements derived from different promoters found in nature, or evencomprise synthetic DNA segments. It is understood by those skilled inthe art that different promoters may direct the expression of a gene indifferent tissues or cell types, or at different stages of development,or in response to different environmental or physiological conditions.Promoters which cause a gene to be expressed in most cell types at mosttimes are commonly referred to as “constitutive promoters”. It isfurther recognized that since in most cases the exact boundaries ofregulatory sequences have not been completely defined, DNA fragments ofdifferent lengths may have identical promoter activity.

The term “operably linked” refers to the association of nucleic acidsequences on a single nucleic acid fragment so that the function of oneis affected by the other. For example, a promoter is operably linkedwith a coding sequence when it is capable of effecting the expression ofthat coding sequence (i.e., that the coding sequence is under thetranscriptional control of the promoter). Coding sequences can beoperably linked to regulatory sequences in sense or antisenseorientation.

The term “expression”, as used herein, refers to the transcription andstable accumulation of sense (mRNA) or antisense RNA derived from thenucleic acid fragment of the invention. Expression may also refer totranslation of mRNA into a polypeptide.

As used herein the term “transformation” refers to the transfer of anucleic acid fragment into a host organism, resulting in geneticallystable inheritance. Host organisms containing the transformed nucleicacid fragments are referred to as “transgenic” or “recombinant” or“transformed” organisms.

The terms “plasmid” and “vector” as used herein, refer to an extrachromosomal element often carrying genes which are not part of thecentral metabolism of the cell, and usually in the form of circulardouble-stranded DNA molecules. Such elements may be autonomouslyreplicating sequences, genome integrating sequences, phage or nucleotidesequences, linear or circular, of a single- or double-stranded DNA orRNA, derived from any source, in which a number of nucleotide sequenceshave been joined or recombined into a unique construction which iscapable of introducing a promoter fragment and DNA sequence for aselected gene product along with appropriate 3′ untranslated sequenceinto a cell.

As used herein the term “codon degeneracy” refers to the nature in thegenetic code permitting variation of the nucleotide sequence withouteffecting the amino acid sequence of an encoded polypeptide. The skilledartisan is well aware of the “codon-bias” exhibited by a specific hostcell in usage of nucleotide codons to specify a given amino acid.Therefore, when synthesizing a gene for improved expression in a hostcell, it is desirable to design the gene such that its frequency ofcodon usage approaches the frequency of preferred codon usage of thehost cell.

The term “codon-optimized” as it refers to genes or coding regions ofnucleic acid molecules for transformation of various hosts, refers tothe alteration of codons in the gene or coding regions of the nucleicacid molecules to reflect the typical codon usage of the host organismwithout altering the polypeptide encoded by the DNA.

As used herein, an “isolated nucleic acid fragment” or “isolated nucleicacid molecule” will be used interchangeably and will mean a polymer ofRNA or DNA that is single- or double-stranded, optionally containingsynthetic, non-natural or altered nucleotide bases. An isolated nucleicacid fragment in the form of a polymer of DNA may be comprised of one ormore segments of cDNA, genomic DNA or synthetic DNA.

A nucleic acid fragment is “hybridizable” to another nucleic acidfragment, such as a cDNA, genomic DNA, or RNA molecule, when asingle-stranded form of the nucleic acid fragment can anneal to theother nucleic acid fragment under the appropriate conditions oftemperature and solution ionic strength. Hybridization and washingconditions are well known and exemplified in Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2^(nd) ed.,Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y. (1989),particularly Chapter 11 and Table 11.1 therein (entirely incorporatedherein by reference). The conditions of temperature and ionic strengthdetermine the “stringency” of the hybridization. Stringency conditionscan be adjusted to screen for moderately similar fragments (such ashomologous sequences from distantly related organisms), to highlysimilar fragments (such as genes that duplicate functional enzymes fromclosely related organisms). Post-hybridization washes determinestringency conditions. One set of preferred conditions uses a series ofwashes starting with 6×SSC, 0.5% SDS at room temperature for 15 min,then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and thenrepeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A morepreferred set of stringent conditions uses higher temperatures in whichthe washes are identical to those above except for the temperature ofthe final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C.Another preferred set of highly stringent conditions uses two finalwashes in 0.1×SSC, 0.1% SDS at 65° C. An additional set of stringentconditions include hybridization at 0.1×SSC, 0.1% SDS, 65° C. and washeswith 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS, for example.

Hybridization requires that the two nucleic acids contain complementarysequences, although depending on the stringency of the hybridization,mismatches between bases are possible. The appropriate stringency forhybridizing nucleic acids depends on the length of the nucleic acids andthe degree of complementation, variables well known in the art. Thegreater the degree of similarity or homology between two nucleotidesequences, the greater the value of Tm for hybrids of nucleic acidshaving those sequences. The relative stability (corresponding to higherTm) of nucleic acid hybridizations decreases in the following order:RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotidesin length, equations for calculating Tm have been derived (see Sambrooket al., supra, 9.50-9.51). For hybridizations with shorter nucleicacids, i.e., oligonucleotides, the position of mismatches becomes moreimportant, and the length of the oligonucleotide determines itsspecificity (see Sambrook et al., supra, 11.7-11.8). In one embodimentthe length for a hybridizable nucleic acid is at least about 10nucleotides. Preferably a minimum length for a hybridizable nucleic acidis at least about 15 nucleotides; more preferably at least about 20nucleotides; and most preferably the length is at least about 30nucleotides. Furthermore, the skilled artisan will recognize that thetemperature and wash solution salt concentration may be adjusted asnecessary according to factors such as length of the probe.

A “substantial portion” of an amino acid or nucleotide sequence is thatportion comprising enough of the amino acid sequence of a polypeptide orthe nucleotide sequence of a gene to putatively identify thatpolypeptide or gene, either by manual evaluation of the sequence by oneskilled in the art, or by computer-automated sequence comparison andidentification using algorithms such as the Basic Local Alignment SearchTool (BLAST; Altschul, S. F., et al., J. Mol. Biol., 215:403-410(1993)). In general, a sequence of ten or more contiguous amino acids orthirty or more nucleotides is necessary in order to putatively identifya polypeptide or nucleic acid sequence as homologous to a known proteinor gene. Moreover, with respect to nucleotide sequences, gene specificoligonucleotide probes comprising 20-30 contiguous nucleotides may beused in sequence-dependent methods of gene identification (e.g.,Southern hybridization) and isolation (e.g., in situ hybridization ofbacterial colonies or bacteriophage plaques). In addition, shortoligonucleotides of 12-15 bases may be used as amplification primers inPCR in order to obtain a particular nucleic acid fragment comprising theprimers. Accordingly, a “substantial portion” of a nucleotide sequencecomprises enough of the sequence to specifically identify and/or isolatea nucleic acid fragment comprising the sequence. The instantspecification teaches the complete amino acid and nucleotide sequenceencoding particular proteins. The skilled artisan, having the benefit ofthe sequences as reported herein, may now use all or a substantialportion of the disclosed sequences for purposes known to those skilledin this art. Accordingly, the instant invention comprises the completesequences as reported in the accompanying Sequence Listing, as well assubstantial portions of those sequences as defined above.

The term “complementary” is used to describe the relationship betweennucleotide bases that are capable of hybridizing to one another. Forexample, with respect to DNA, adenosine is complementary to thymine andcytosine is complementary to guanine.

The term “percent identity”, as known in the art, is a relationshipbetween two or more polypeptide sequences or two or more polynucleotidesequences, as determined by comparing the sequences. In the art,“identity” also means the degree of sequence relatedness betweenpolypeptide or polynucleotide sequences, as the case may be, asdetermined by the match between strings of such sequences. “Identity”and “similarity” can be readily calculated by known methods, includingbut not limited to those described in: 1.) Computational MolecularBiology (Lesk, A. M., Ed.) Oxford University: NY (1988); 2.)Biocomputing: Informatics and Genome Projects (Smith, D. W., Ed.)Academic: NY (1993); 3.) Computer Analysis of Sequence Data, Part I(Griffin, A. M., and Griffin, H. G., Eds.) Humania: NJ (1994); 4.)Sequence Analysis in Molecular Biology (von Heinje, G., Ed.) Academic(1987); and 5.) Sequence Analysis Primer (Gribskov, M. and Devereux, J.,Eds.) Stockton: NY (1991).

Preferred methods to determine identity are designed to give the bestmatch between the sequences tested. Methods to determine identity andsimilarity are codified in publicly available computer programs.Sequence alignments and percent identity calculations may be performedusing the MegAlign™ program of the LASERGENE bioinformatics computingsuite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequencesis performed using the “Clustal method of alignment” which encompassesseveral varieties of the algorithm including the “Clustal V method ofalignment” corresponding to the alignment method labeled Clustal V(described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D.G. et al., Comput. Appl. Biosci., 8:189-191 (1992)) and found in theMegAlign™ program of the LASERGENE bioinformatics computing suite(DNASTAR Inc.). For multiple alignments, the default values correspondto GAP PENALTY=10 and GAP LENGTH PENALTY=10. Default parameters forpairwise alignments and calculation of percent identity of proteinsequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2,GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4. After alignment of thesequences using the Clustal V program, it is possible to obtain a“percent identity” by viewing the “sequence distances” table in the sameprogram. Additionally the “Clustal W method of alignment” is availableand corresponds to the alignment method labeled Clustal W (described byHiggins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al.,Comput. Appl. Biosci. 8:189-191(1992)) and found in the MegAlign™ v 6.1program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.).Default parameters for multiple alignment (GAP PENALTY=10, GAP LENGTHPENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5,Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB). Afteralignment of the sequences using the Clustal W program, it is possibleto obtain a “percent identity” by viewing the “sequence distances” tablein the same program.

It is well understood by one skilled in the art that many levels ofsequence identity are useful in identifying polypeptides, from otherspecies, wherein such polypeptides have the same or similar function oractivity. Useful examples of percent identities include, but are notlimited to: 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%, or anyinteger percentage from 55% to 100% may be useful in describing thepresent invention, such as 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%,64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%,78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%. Suitable nucleic acidfragments not only have the above homologies but typically encode apolypeptide having at least 50 amino acids, preferably at least 100amino acids, more preferably at least 150 amino acids, still morepreferably at least 200 amino acids, and most preferably at least 250amino acids.

The term “sequence analysis software” refers to any computer algorithmor software program that is useful for the analysis of nucleotide oramino acid sequences. “Sequence analysis software” may be commerciallyavailable or independently developed. Typical sequence analysis softwarewill include, but is not limited to: 1.) the GCG suite of programs(Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison,Wis.); 2.) BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol.,215:403-410 (1990)); 3.) DNASTAR software (DNASTAR, Inc. Madison, Wis.);4.) SEQUENCHER software (Gene Codes Corporation, Ann Arbor, Mich.); and5.) the FASTA program incorporating the Smith-Waterman algorithm (W. R.Pearson, Comput. Methods Genome Res., [Proc. Int. Symp.] (1994), MeetingDate 1992, 111-20. Editor(s): Suhai, Sandor. Plenum: New York, N.Y.).Within the context of this application it will be understood that wheresequence analysis software is used for analysis, that the results of theanalysis will be based on the “default values” of the programreferenced, unless otherwise specified. As used herein “default values”will mean any set of values or parameters that originally load with thesoftware when first initialized.

Standard recombinant DNA and molecular cloning techniques used here arewell known in the art and are described by Sambrook, J., Fritsch, E. F.and Maniatis, T., Molecular Cloning: A Laboratory Manual, SecondEdition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(1989) (hereinafter “Maniatis”); and by Silhavy, T. J., Bennan, M. L.and Enquist, L. W., Experiments with Gene Fusions, Cold Spring HarborLaboratory Press, Cold Spring Harbor, N.Y. (1984); and by Ausubel, F. M.et al., Current Protocols in Molecular Biology, published by GreenePublishing Assoc. and Wiley-Interscience (1987). Additional methods usedhere are in Methods in Enzymology, Volume 194, Guide to Yeast Geneticsand Molecular and Cell Biology (Part A, 2004, Christine Guthrie andGerald R. Fink (Eds.), Elsevier Academic Press, San Diego, Calif.).

Discovery of Improved Fe—S Cluster Protein Activity in Yeast

Proteins that contain a bound iron-sulfur cluster (Fe—S) that isrequired for their activity may have low activity when used inheterologous expression systems. Formation of Fe—S clusters and theirtransfer to apo-proteins is a multistep process involving at leastseveral proteins including cysteine desulfurase, a scaffold protein anda chaperone. Thus a heterologous Fe—S protein may not be effectivelycomposed by the endogenous host system. Applicants have discovered a wayto increase activity of an Fe—S protein expressed as a heterologousprotein in a yeast host cell. Applicants have found that by reducingproduction of an endogenous Fe—S protein in the yeast host cell, animprovement in activity of an expressed heterologous Fe—S clusterprotein can be achieved. Expression in yeast of either heterologousfungal or plant 2Fe-2S dihydroxy-acid dehydratase (DHAD) or Fe—Spropanediol dehydratase reactivase (RdhtB) was improved when anendogenous gene encoding isopropylmalate dehydratase (LEU1) or anendogenous gene encoding dihydroxy-acid dehydratase (ILV3) wasinactivated in the yeast host cells.

In yeast host cells with inactivation of a gene encoding an endogenousFe—S protein, the activity of the expressed heterologous Fe—S proteinmay be increased to at least about 1.4 fold of the activity in a yeasthost cell with no inactivation of Fe—S protein encoding gene. Forexample, the Kluyveromyces lactis DHAD had 1.4 fold activity in a LEU1deletion host as compared to a host without the deletion; the Roseburiainulinivorans RdhtB had 1.7 fold comparative activity in a LEU deletionhost as measured by the activated RdhtA protein activity (describedbelow); Saccharomyces cerevisiae DHAD expressed in the cytosol had 1.5fold comparative activity in a mitochondrial ILV3 deletion host; andKluyveromyces lactis DHAD expressed in the cytosol had 7.4 foldcomparative activity in a mitochondrial ILV3 deletion host.

Yeast Host Cells with Reduced Expression of Endogenous Fe—S Protein

Reduced endogenous Fe—S protein expression may be engineered in anyyeast cell that is amenable to genetic manipulation. Examples includeyeasts of Saccharomyces, Schizosaccharomyces, Hansenula, Candida,Kluyveromyces, Yarrowia and Pichia. Suitable strains include, but arenot limited to, Saccharomyces cerevisiae, Schizosaccharomyces pombe,Kluyveromyces lactis, Kluyveromyces thermotolerans, Candida glabrata,Candida albicans, Pichia stipitis and Yarrowia lipolytica. Particularlysuitable is Saccharomyces cerevisiae.

In any of these yeasts, any endogenous Fe—S protein may be a target forreduced expression. Fe—S proteins in yeast that may be targeted forreduced expression include, for example, the following proteins (withencoding gene): aconitase (ACO1), homoaconitase (LYS4), DHAD (ILV3),lipoate synthase (LIPS), biotin synthase (BIO2), ferredoxin maturation(YAH1), NADH ubiquinone oxidoreductase (NDI1), succinate dehydrogenase(SDH2), ubiquinol-cytochrome-c reductase (RIP1), isopropylmalateisomerase (LEU1), sulfite reductase (ECM17), glutamate dehydrogenase(GLT1), ABC protein Rli1 (RLI1), NTPase Nbp35 (NBP35), andhydrogenase-like protein (NARI1). Yeast cells with reduced expression ofindividual Fe—S proteins may require special conditions for growth suchas supplementation of the growth medium with a particular nutrient, asis well known to one skilled in the art. For example, a strain withdisruption of LEU1 is supplemented with leucine, a strain withdisruption of DHAD is supplemented with leucine, isoleucine, and valine,and a strain with disruption of LYS4 is supplemented with lysine. Somestrains with a disruption require no supplementation for growth.Particularly suitable Fe—S proteins that may be targeted for reducedexpression include Isopropylmalate isomerase (LEU1), Dihydroxyaciddehydratase (ILV3), Sulfite reductase (ECM17), Glutamate dehydrogenase(GLT1), and Biotin synthase (BIO2). Reduced expression is engineered forat least one endogenous Fe—S protein, and two or more endogenous Fe—Sproteins may be reduced.

LEU1 encodes isopropylmalate dehydratase, an enzyme belonging to EC4.2.1.33 that is involved in branched chain amino acid biosynthesis,specifically synthesis of leucine. Any gene encoding an isopropylmalatedehydratase, which is an enzyme requiring a 4Fe-4S cluster for activity,may be inactivated in a yeast host cell of this disclosure. Examples ofyeast LEU1 inactivation target genes and their encoded proteins arethose from Saccharomyces cerevisiae (coding SEQ ID NO:1; protein SEQ IDNO:2), Schizosaccharomyces pombe (coding SEQ ID NO:3; protein SEQ IDNO:4), Candida galbrata strain CBS 138 (coding SEQ ID NO:5; protein SEQID NO:6), Candida albicans SC5314 (coding SEQ ID NO:7; protein SEQ IDNO:8), Kluyveromyces lactis (coding SEQ ID NO: protein SEQ ID NO:10),Yarrowia lipolytica (coding SEQ ID NO:11; protein SEQ ID NO:12) andPichia stipitis (coding SEQ ID NO:13; protein SEQ ID NO:14).

Similarly in any of the yeast hosts described herein, an endogenous ILV3gene may be inactivated to reduce endogenous Fe—S protein expression.ILV3 encodes mitochondrial DHAD that is involved in branched chain aminoacid biosynthesis. Mitochondrial DHAD is encoded by a nuclear gene, andhas a mitochondrial targeting signal sequence so that it is transportedto and localized in the mitochondrion. Any ILV3 gene may be inactivatedin a yeast host cell of this disclosure. Examples of yeast ILV3inactivation target genes and their encoded proteins are those fromSaccharomyces cerevisiae YJM78 (coding SEQ ID NO:111; protein SEQ IDNO:112), Schizosaccharomyces pombe (coding SEQ ID NO:93; protein SEQ IDNO:94), Candida galbrata strain CBS 138 (coding SEQ ID NO:107; proteinSEQ ID NO:108), Candida albicans SC5314 (coding SEQ ID NO:101; proteinSEQ ID NO:102), Kluyveromyces lactis (coding SEQ ID NO:113; protein SEQID NO:114), Yarrowia lipolytica (coding SEQ ID NO:105; protein SEQ IDNO:106) and Pichia stipitis CBS 6054 (coding SEQ ID NO:103; protein SEQID NO:104).

Because genes encoding isopropylmalate dehydratases and DHAD enzymesgenes are well known, and because of the prevalence of genomicsequencing, additional suitable species of these enzymes can be readilyidentified by one skilled in the art on the basis of sequence similarityusing bioinformatics approaches. Typically BLAST (described above)searching of publicly available databases with known isopropylmalatedehydratase amino acid sequences, such as those provided herein, is usedto identify these enzymes and their encoding sequences that may betargeted for inactivation in the present strains. For example,endogenous yeast isopropylmalate dehydratase and DHAD proteins havingamino acid sequence identities of at least about 70-75%, 75%-80%,80-85%, 85%-90%, 90%-95% or 98% sequence identity to any of theisopropylmalate dehydratase proteins of SEQ ID NOs:2, 4, 6, 8, 10, 12and 14 and the DHAD proteins of SEQ ID NOs:94, 102, 104, 106, 108, 112,and 114 may have reduced expression in the present strains. Identitiesare based on the Clustal W method of alignment using the defaultparameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250series of protein weight matrix.

Additionally, the sequences of LEU1 coding regions and ILV3 providedherein may be used to identify other homologs in nature. For exampleeach of the coding regions described herein may be used to isolate genesencoding homologous proteins. Isolation of homologous genes usingsequence-dependent protocols is well known in the art. Examples ofsequence-dependent protocols include, but are not limited to: 1.)methods of nucleic acid hybridization; 2.) methods of DNA and RNAamplification, as exemplified by various uses of nucleic acidamplification technologies [e.g., polymerase chain reaction (PCR),Mullis et al., U.S. Pat. No. 4,683,202; ligase chain reaction (LCR),Tabor, S. et al., Proc. Acad. Sci. USA 82:1074 (1985); or stranddisplacement amplification (SDA), Walker, et al., Proc. Natl. Acad. Sci.U.S.A., 89:392 (1992)]; and 3.) methods of library construction andscreening by complementation.

For example, genes encoding similar proteins or polypeptides to theisopropylmalate dehydratase and DHAD encoding genes provided hereincould be isolated directly by using all or a portion of the instantnucleic acid fragments as DNA hybridization probes to screen librariesfrom any desired organism using methodology well known to those skilledin the art. Specific oligonucleotide probes based upon the disclosednucleic acid sequences can be designed and synthesized by methods knownin the art (Maniatis, supra). Moreover, the entire sequences can be useddirectly to synthesize DNA probes by methods known to the skilledartisan (e.g., random primers DNA labeling, nick translation orend-labeling techniques), or RNA probes using available in vitrotranscription systems. In addition, specific primers can be designed andused to amplify a part of (or full-length of) the instant sequences. Theresulting amplification products can be labeled directly duringamplification reactions or labeled after amplification reactions, andused as probes to isolate full-length DNA fragments by hybridizationunder conditions of appropriate stringency.

Typically, in PCR-type amplification techniques, the primers havedifferent sequences and are not complementary to each other. Dependingon the desired test conditions, the sequences of the primers should bedesigned to provide for both efficient and faithful replication of thetarget nucleic acid. Methods of PCR primer design are common and wellknown in the art (Thein and Wallace, “The use of oligonucleotides asspecific hybridization probes in the Diagnosis of Genetic Disorders”, inHuman Genetic Diseases: A Practical Approach, K. E. Davis Ed., (1986) pp33-50, IRL: Herndon, V A; and Rychlik, W., In Methods in MolecularBiology, White, B. A. Ed., (1993) Vol. 15, pp 31-39, PCR Protocols:Current Methods and Applications. Humania: Totowa, N.J.).

Generally two short segments of the described sequences may be used inpolymerase chain reaction protocols to amplify longer nucleic acidfragments encoding homologous genes from DNA or RNA. The polymerasechain reaction may also be performed on a library of cloned nucleic acidfragments wherein the sequence of one primer is derived from thedescribed nucleic acid fragments, and the sequence of the other primertakes advantage of the presence of the polyadenylic acid tracts to the3′ end of the mRNA precursor encoding microbial genes.

Alternatively, the second primer sequence may be based upon sequencesderived from the cloning vector. For example, the skilled artisan canfollow the RACE protocol (Frohman et al., PNAS USA 85:8998 (1988)) togenerate cDNAs by using PCR to amplify copies of the region between asingle point in the transcript and the 3′ or 5′ end. Primers oriented inthe 3′ and 5′ directions can be designed from the instant sequences.Using commercially available 3′ RACE or 5′ RACE systems (e.g., BRL,Gaithersburg, Md.), specific 3′ or 5′ cDNA fragments can be isolated(Ohara et al., PNAS USA 86:5673 (1989); Loh et al., Science 243:217(1989)).

Alternatively, the provided isopropylmalate dehydratase and DHADencoding sequences can be employed as hybridization reagents for theidentification of homologs. The basic components of a nucleic acidhybridization test include a probe, a sample suspected of containing thegene or gene fragment of interest, and a specific hybridization method.Probes are typically single-stranded nucleic acid sequences that arecomplementary to the nucleic acid sequences to be detected. Probes are“hybridizable” to the nucleic acid sequence to be detected. The probelength can vary from 5 bases to tens of thousands of bases, and willdepend upon the specific test to be done. Typically a probe length ofabout 15 bases to about 30 bases is suitable. Only part of the probemolecule need be complementary to the nucleic acid sequence to bedetected. In addition, the complementarity between the probe and thetarget sequence need not be perfect. Hybridization does occur betweenimperfectly complementary molecules with the result that a certainfraction of the bases in the hybridized region are not paired with theproper complementary base.

Hybridization methods are well defined. Typically the probe and samplemust be mixed under conditions that will permit nucleic acidhybridization. This involves contacting the probe and sample in thepresence of an inorganic or organic salt under the proper concentrationand temperature conditions. The probe and sample nucleic acids must bein contact for a long enough time that any possible hybridizationbetween the probe and sample nucleic acid may occur. The concentrationof probe or target in the mixture will determine the time necessary forhybridization to occur. The higher the probe or target concentration,the shorter the hybridization incubation time needed. Optionally, achaotropic agent may be added. The chaotropic agent stabilizes nucleicacids by inhibiting nuclease activity. Furthermore, the chaotropic agentallows sensitive and stringent hybridization of short oligonucleotideprobes at room temperature (Van Ness and Chen, Nucl. Acids Res.19:5143-5151 (1991)). Suitable chaotropic agents include guanidiniumchloride, guanidinium thiocyanate, sodium thiocyanate, lithiumtetrachloroacetate, sodium perchlorate, rubidium tetrachloroacetate,potassium iodide and cesium trifluoroacetate, among others. Typically,the chaotropic agent will be present at a final concentration of about 3M. If desired, one can add formamide to the hybridization mixture,typically 30-50% (v/v).

Various hybridization solutions can be employed. Typically, thesecomprise from about 20 to 60% volume, preferably 30%, of a polar organicsolvent. A common hybridization solution employs about 30-50% v/vformamide, about 0.15 to 1 M sodium chloride, about 0.05 to 0.1 Mbuffers (e.g., sodium citrate, Tris-HCl, PIPES or HEPES (pH range about6-9)), about 0.05 to 0.2% detergent (e.g., sodium dodecylsulfate), orbetween 0.5-20 mM EDTA, FICOLL polymer (Pharmacia Inc.) (about 300-500kdal), polyvinylpyrrolidone (about 250-500 kdal) and serum albumin. Alsoincluded in the typical hybridization solution will be unlabeled carriernucleic acids from about 0.1 to 5 mg/mL, fragmented nucleic DNA (e.g.,calf thymus or salmon sperm DNA, or yeast RNA), and optionally fromabout 0.5 to 2% wt/vol glycine. Other additives may also be included,such as volume exclusion agents that include a variety of polarwater-soluble or swellable agents (e.g., polyethylene glycol), anionicpolymers (e.g., polyacrylate or polymethylacrylate) and anionicsaccharidic polymers (e.g., dextran sulfate).

Nucleic acid hybridization is adaptable to a variety of assay formats.One of the most suitable is the sandwich assay format. The sandwichassay is particularly adaptable to hybridization under non-denaturingconditions. A primary component of a sandwich-type assay is a solidsupport. The solid support has adsorbed to it or covalently coupled toit immobilized nucleic acid probe that is unlabeled and complementary toone portion of the sequence.

Protein and nucleic acid encoding sequences for any of the other Fe—Sproteins that may be targeted for reduced activity in a yeast cell ofthe invention may be identified using bioinformatics and other methodswell known to one skilled in the art. For example, aconitase sequencesare identified by keyword searching in bioinformatics databases. Severalsequences identified by this method are those from Saccharomycescerevisiae (coding SEQ ID NO:153; protein SEQ ID NO:154),Schizosaccharomyces pombe on chromosome II (coding SEQ ID NO:155;protein SEQ ID NO:156), Schizosaccharomyces pombe on chromosome I(coding SEQ ID NO:157; protein SEQ ID NO:158), Kluyveromyces lactis(coding SEQ ID NO:15; protein SEQ ID NO:160), Candida albicans SC5314(coding SEQ ID NO:161; protein SEQ ID NO:162), Yarrowia lipolytica(coding SEQ ID NO:163; protein SEQ ID NO:164), Pichia stipitis CBS 6054(coding SEQ ID NO:165; protein SEQ ID NO:166), Candida galbrata CBS 138chromosome F (coding SEQ ID NO:167; protein SEQ ID NO:168), Candidagalbrata CBS 138 chromosome D (coding SEQ ID NO:169; protein SEQ IDNO:170), and Candida galbrata CBS 138 chromosome K (coding SEQ IDNO:171; protein SEQ ID NO:172).

Genes encoding Fe—S proteins, for example LEU1, ILV3, or ACO1 may bedisrupted in any yeast cell using genetic modification. Many methods forgenetic modification of target genes are known to one skilled in the artand may be used to create the present yeast strains. Modifications thatmay be used to reduce or eliminate expression of a target protein aredisruptions that include, but are not limited to, deletion of the entiregene or a portion of the gene, inserting a DNA fragment into the gene(in either the promoter or coding region) so that the protein is notexpressed or expressed at lower levels, introducing a mutation into thecoding region which adds a stop codon or frame shift such that afunctional protein is not expressed, and introducing one or moremutations into the coding region to alter amino acids so that anon-functional or a less enzymatically active protein is expressed. Inaddition, expression of a gene may be blocked by expression of anantisense RNA or an interfering RNA, and constructs may be introducedthat result in cosuppression. In addition, the synthesis or stability ofthe transcript may be lessened by mutation. Similarly the efficiency bywhich a protein is translated from mRNA may be modulated by mutation.All of these methods may be readily practiced by one skilled in the artmaking use of the known or identified coding sequences such as LEU1 orILV3.

DNA sequences surrounding a LEU1, ILV3, or ACO1 coding sequence are alsouseful in some modification procedures and are available for yeasts suchas for Saccharomycse cerevisiae in the complete genome sequencecoordinated by Genome Project ID9518 of Genome Projects coordinated byNCBI (National Center for Biotechnology Information) with identifyingGOPID 13838. Additional examples of yeast genomic sequences include thatof Yarrowia lipolytica, GOPIC 13837, and of Candida albicans, which isincluded in GPID 10771, 10701 and 16373. Additional genomes have beencompletely sequenced and annotated and are publicly available for thefollowing yeast strains Candida glabrata CBS 138, Kluyveromyces lactisNRRL Y-1140, Pichia stipitis CBS 6054, and Schizosaccharomyces pombe972h-.

In particular, DNA sequences surrounding a target coding sequence, suchas LEU1 or ILV3, are useful for modification methods using homologousrecombination. For example, in this method flanking sequences are placedbounding a selectable marker gene to mediate homologous recombinationwhereby the marker gene replaces the target gene. Also partial targetgene sequences and flanking sequences bounding a selectable marker genemay be used to mediate homologous recombination whereby the marker genereplaces a portion of the target gene. In addition, the selectablemarker may be bounded by site-specific recombination sites, so thatfollowing expression of the corresponding site-specific recombinase, theresistance gene is excised from the target gene without reactivating thelatter. The site-specific recombination leaves behind a recombinationsite which disrupts expression of the target gene encoded protein. Thehomologous recombination vector may be constructed to also leave adeletion in the target gene following excision of the selectable marker,as is well known to one skilled in the art.

Deletions may be made using mitotic recombination as described in Wachet al. ((1994) Yeast 10:1793-1808). This method involves preparing a DNAfragment that contains a selectable marker between genomic regions thatmay be as short as 20 bp, and which bound a target DNA sequence. ThisDNA fragment can be prepared by PCR amplification of the selectablemarker gene using as primers oligonucleotides that hybridize to the endsof the marker gene and that include the genomic regions that canrecombine with the yeast genome. The linear DNA fragment can beefficiently transformed into yeast and recombined into the genomeresulting in gene replacement including with deletion of the target DNAsequence (as described in Methods in Enzymology, v 194, pp 281-301(1991)).

Moreover, promoter replacement methods may be used to exchange theendogenous transcriptional control elements allowing another means tomodulate expression such as described in Mnaimneh et al. ((2004) Cell118(1):31-44) and in Example 12 herein.

In addition, a target gene in any yeast cell may be disrupted usingrandom mutagenesis, which is followed by screening to identify strainswith reduced target gene encided activity. Using this type of method,the DNA sequence of for example the LEU1, ILV3, or any other region ofthe genome affecting expression of a target Fe—S protein, need not beknown.

Methods for creating genetic mutations are common and well known in theart and may be applied to the exercise of creating mutants. Commonlyused random genetic modification methods (reviewed in Methods in YeastGenetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor,N.Y.) include spontaneous mutagenesis, mutagenesis caused by mutatorgenes, chemical mutagenesis, irradiation with UV or X-rays, ortransposon mutagenesis.

Chemical mutagenesis of yeast commonly involves treatment of yeast cellswith one of the following DNA mutagens: ethyl methanesulfonate (EMS),nitrous acid, diethyl sulfate, or N-methyl-N′-nitro-N-nitroso-guanidine(MNNG). These methods of mutagenesis have been reviewed in Spencer et al(Mutagenesis in Yeast, 1996, Yeast Protocols: Methods in Cell andMolecular Biology. Humana Press, Totowa, N.J.). Chemical mutagenesiswith EMS may be performed as described in Methods in Yeast Genetics,2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.Irradiation with ultraviolet (UV) light or X-rays can also be used toproduce random mutagenesis in yeast cells. The primary effect ofmutagenesis by UV irradiation is the formation of pyrimidine dimerswhich disrupt the fidelity of DNA replication. Protocols forUV-mutagenesis of yeast can be found in Spencer et al (Mutagenesis inYeast, 1996, Yeast Protocols: Methods in Cell and Molecular Biology.Humana Press, Totowa, N.J.). Introduction of a mutator phenotype canalso be used to generate random chromosomal mutations in yeast. Commonmutator phenotypes can be obtained through disruption of one or more ofthe following genes: PMS1, MAG1, RAD18 or RAD51. Restoration of thenon-mutator phenotype can be easily obtained by insertion of thewildtype allele. Collections of modified cells produced from any ofthese or other known random mutagenesis processes may be screened forreduced Fe—S protein activity.

Heterologous Fe—S Proteins

Any fungal or plant 2Fe-2S cluster dihydroxy-acid dehydratase (DHAD) andany Fe—S propanediol dehydratase reactivase may be expressed as aheterologous protein in a yeast host cell engineered as disclosed hereinfor reduced endogenous Fe—S cluster protein expression, and increasedactivity may be obtained. A heterologous protein includes one that isexpressed in a manner differently from expression of a correspondingendogenous protein. For example in yeast, endogenous DHAD is encoded byILV3 in the nucleus and the expressed DHAD protein has a mitochondrialtargeting signal sequence such that the protein is localized in themitochondrion. An Fe—S cluster is added to the DHAD protein in themitochondrion for its activity in branched chain amino acidbiosynthesis. It is desirable to express DHAD activity in the cytosolfor participation in biosynthetic pathways that are localized in thecytosol. Cytosolic expression of DHAD in yeast is heterologousexpression since the native protein is localized in the mitochondrion.For example, heterologous expression of the Saccharomyces cerevisiaeDHAD in S. cerevisiae is obtained by expressing the S. cerevisiae DHADcoding region with the mitochondrial targeting signal removed, such thatthe protein remains in the cytosol. 2Fe-2S DHADs that may be used in thepresent disclosure include those from fungi and plants. Representativefungal or plant 2Fe-2S DHADs are listed in Tables 1 and 2. Fungal orplant 2Fe-2S DHADs with amino acid sequence identities of 95% or greaterwere removed from the analysis providing this list for simplification.However, any sequences with 95% or greater amino acid identities to anyof these sequences are useful in the present invention. The analysisused to obtain 2Fe-2S DHADs is described in commonly owned andco-pending U.S. Patent Application 61/100,792, which is hereinincorporated by reference. The analysis is as follows: Therein a ProfileHidden Markov Model (HMM) was prepared based on amino acid sequences ofeight functionally verified DHADs. These DHADs are from Nitrosomonaseuropaea (DNA SEQ ID NO:174; Protein SEQ ID NO:175), Synechocystis sp.PCC6803 (DNA SEQ ID:176; Protein SEQ ID NO:177), Streptococcus mutans(DNA SEQ ID NO:178; Protein SEQ ID NO:179), Streptococcus thermophilus(DNA SEQ ID NO:180; protein SEQ ID No:181), Ralstonia metallidurans (DNASEQ ID NO:182; protein SEQ ID NO:183), Ralstonia eutropha (DNA SEQ IDNO:184; protein SEQ ID NO:185), and Lactococcus lactis (DNA SEQ IDNO:186; protein SEQ ID NO:187). In addition the DHAD from Flavobacteriumjohnsoniae (DNA SEQ ID NO:188; protein SEQ ID NO:189) was found to havedihydroxy-acid dehydratase activity when expressed in E. coli and wasused in making the Profile. The Profile HMM is prepared using the HMMERsoftware package (The theory behind profile HMMs is described in R.Durbin, S. Eddy, A. Krogh, and G. Mitchison, Biological sequenceanalysis: probabilistic models of proteins and nucleic acids, CambridgeUniversity Press, 1998; Krogh et al., 1994; J. Mol. Biol.235:1501-1531), following the user guide which is available from HMMER(Janelia Farm Research Campus, Ashburn, Va.). The output of the HMMERsoftware program is a Profile Hidden Markov Model (HMM) thatcharacterizes the input sequences, given in Table 9.

Any protein that matches the Profile HMM with an E value of <10⁻⁵ is aDHAD related protein, which includes 4Fe-4S DHADs, 2Fe-2S DHADs,arabonate dehydratases, and phosphogluconate dehydratases. Sequencesmatching the Profile HMM are then analyzed for the presence of the threeconserved cysteines, corresponding to positions 56, 129, and 201 in theStreptococcus mutans DHAD. The presence of all three conserved cysteinesis characteristic of proteins having a [2Fe-2S]²⁺ cluster. Proteinshaving the three conserved cysteines include arabonate dehydratases and2Fe-2S DHADs. The 2Fe-2S DHADs may be distinguished from the arabonatedehydratases by analyzing for signature conserved amino acids found tobe present in the 2Fe-2S DHADs or in the arabonate dehydratases atpositions corresponding to the following positions in the Streptococcusmutans DHAD amino acid sequence. These signature amino acids are in2Fe-2S DHADs or in arabonate dehydratases, respectively, at thefollowing positions (with greater than 90% occurrence): 88 asparagine vsglutamic acid; 113 not conserved vs glutamic acid; 142 arginine orasparagine vs not conserved; 165: not conserved vs glycine; 208asparagine vs not conserved; 454 leucine vs not conserved; 477phenylalanine or tyrosine vs not conserved; and 487 glycine vs notconserved.

The proteins identified by this process that have a fungal or plantorigin, such as SEQ ID NOs:46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66,68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100,102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128,130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150 and 152 may beused in the present invention, as well as any protein with amino acididentity of at least about 95%, 96%, 97%, 98%, or 99% to any of thesesequences. Particularly suitable is the DHAD from Kluyveromyces lactis(SEQ ID NO:114) and DHADs with at least about 90% amino acid sequenceidentity to SEQ ID NO:114 using the Clustal W method of alignment usingthe default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, andGonnet 250 series of protein weight matrix over the full length of theprotein sequence.

In addition, fungal or plant 2Fe-2S DHADs that may be used in thepresent invention may be identified by their position in a fungal orplant 2Fe-2S DHAD branch of a phylogenetic tree of DHAD relatedproteins. In addition, 2Fe-2S DHADs that may be used may be identifiedusing sequence comparisons with any of the fungal or plant 2Fe-2S DHADswhose sequences are provided herein, where sequence identity may be atleast about 80%-85%, 85%-90%, 90%-95% or 95%-99%.

Additionally, the sequences of fungal or plant 2Fe-2S DHADs providedherein may be used to identify other homologs in nature. For exampleeach of the DHAD encoding nucleic acid fragments given herein as SEQ IDNOs:45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77,79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109,111, 113, 115, 117, 11, 121, 123, 125, 127, 129, 131, 133, 135, 137, 13,141, 143, 145, 147, 149 and 151 may be used to isolate genes encodinghomologous proteins as described above for the LEU1 coding region.

The coenzyme B12-independent propanediol dehydratase reactivase ofRoseburia inulinivorans is a protein requiring an Fe—S cluster foractivity. This protein, RdhtB, is disclosed in co-pending US Patent PubNo. US20090155870, which is herein incorporated by reference. RdhtBreactivates a coenzyme B12-independent propanediol dehydratase ofRoseburia inulinivorans, which is named RdhtA and is also disclosed incommonly owned and co-pending US Patent Pub No. US20090155870. Theactivity of RdhtB may be assessed by assaying the activity of RdhtA,since RdhtB is required for RdhtA activity. Activity of RdhtB, andtherefore of RdhtA, is improved by expressing in a yeast host withreduced endogenous Fe—S protein expression disclosed herein.Heterologous expression of any coenzyme B12-independent propanedioldehydratase reactivase that requires an Fe—S cluster for activity may beimproved in a yeast strain having reduced endogenous Fe—S proteinexpression. A coenzyme B12-independent propanediol dehydratasereactivase may be readily identified by one skilled in the art byassessing propanediol dehydratase activity of the associated propanedioldehydratase enzyme in the presence or absence of coenzyme B12. Anexample is a diol dehydratase reactivase of Clostridium butyricum(coding region SEQ ID NO:192; protein SEQ ID NO:193).

Other coenzyme B12-independent propanediol dehydratase reactivases thatmay be used may be identified through bioinformatics analysis ofsequences as compared to that of RdhtB SEQ ID NO:44 by one skilled inthe art. Proteins with coenzyme B12-independent propanediol dehydratasereactivase activity and sequence identity to SEQ ID NO:44 of at leastabout 80%-85%, 85%-90%, 90%-95% or 95%-99% may be used. Particularlysuitable are those that are at least about 90% identical to the aminoacid sequence as set forth in SEQ ID NO:44 using the Clustal W method ofalignment using the default parameters of GAP PENALTY=10, GAP LENGTHPENALTY=0.1, and Gonnet 250 series of protein weight matrix over thefull length of the protein sequence.

In addition, other coenzyme B12-independent propanediol dehydratasereactivase homologs that may be used may be identified using the RdhtBcoding region (SEQ ID NO:16) by methods as described above for the LEU1coding region.

Expression of Heterologous Fe—S Proteins

Expression is achieved by transforming with a sequence encoding an Fe—Sprotein. The coding region to be expressed may be codon optimized forthe target host cell, as well known to one skilled in the art. Methodsfor gene expression in yeast are known in the art (see for exampleMethods in Enzymology, Volume 194, Guide to Yeast Genetics and Molecularand Cell Biology (Part A, 2004, Christine Guthrie and Gerald R. Fink(Eds.), Elsevier Academic Press, San Diego, Calif.). Expression of genesin yeast typically requires a promoter, operably linked to a codingregion of interest, and a transcriptional terminator. A number of yeastpromoters can be used in constructing expression cassettes for genes inyeast, including, but not limited to promoters derived from thefollowing genes: CYC1, HIS3, GAL1, GAL10, ADH1, PGK, PHO5, GAPDH, ADC1,TRP1, URA3, LEU2, ENO, TPI, CUP1, FBA, GPD, GPM, and AOX1. Suitabletranscriptional terminators include, but are not limited to FBAt, GPDt,GPMt, ERG10t, GAL1t, CYC1, and ADH1.

Suitable promoters, transcriptional terminators, and coding regions maybe cloned into E. coli-yeast shuttle vectors, and transformed into yeastcells. These vectors allow strain propagation in both E. coli and yeaststrains. Typically the vector used contains a selectable marker andsequences allowing autonomous replication or chromosomal integration inthe desired host. Typically used plasmids in yeast are shuttle vectorspRS423, pRS424, pRS425, and pRS426 (American Type Culture Collection,Manassas, Va.), which contain an E. coli replication origin (e.g.,pMB1), a yeast 2 origin of replication, and a marker for nutritionalselection. The selection markers for these four vectors are His3 (vectorpRS423), Trp1 (vector pRS424), Leu2 (vector pRS425) and Ura3 (vectorpRS426). Construction of expression vectors with a chimeric geneencoding the described Fe—S protein coding regions may be performed byeither standard molecular cloning techniques in E. coli or by the gaprepair recombination method in yeast.

The gap repair cloning approach takes advantage of the highly efficienthomologous recombination in yeast. Typically, a yeast vector DNA isdigested (e.g., in its multiple cloning site) to create a “gap” in itssequence. A number of insert DNAs of interest are generated that containa ≧21 bp sequence at both the 5′ and the 3′ ends that sequentiallyoverlap with each other, and with the 5′ and 3′ terminus of the vectorDNA. For example, to construct a yeast expression vector for “Gene X’, ayeast promoter and a yeast terminator are selected for the expressioncassette. The promoter and terminator are amplified from the yeastgenomic DNA, and Gene X is either PCR amplified from its source organismor obtained from a cloning vector comprising Gene X sequence. There isat least a 21 bp overlapping sequence between the 5′ end of thelinearized vector and the promoter sequence, between the promoter andGene X, between Gene X and the terminator sequence, and between theterminator and the 3′ end of the linearized vector. The “gapped” vectorand the insert DNAs are then co-transformed into a yeast strain andplated on the medium containing the appropriate compound mixtures thatallow complementation of the nutritional selection markers on theplasmids. The presence of correct insert combinations can be confirmedby PCR mapping using plasmid DNA prepared from the selected cells. Theplasmid DNA isolated from yeast (usually low in concentration) can thenbe transformed into an E. coli strain, e.g. TOP10, followed by minipreps and restriction mapping to further verify the plasmid construct.Finally the construct can be verified by sequence analysis.

Like the gap repair technique, integration into the yeast genome alsotakes advantage of the homologous recombination system in yeast.Typically, a cassette containing a coding region plus control elements(promoter and terminator) and auxotrophic marker is PCR-amplified with ahigh-fidelity DNA polymerase using primers that hybridize to thecassette and contain 40-70 base pairs of sequence homology to theregions 5′ and 3′ of the genomic area where insertion is desired. ThePCR product is then transformed into yeast and plated on mediumcontaining the appropriate compound mixtures that allow selection forthe integrated auxotrophic marker. For example, to integrate “Gene X”into chromosomal location “Y”, the promoter-coding regionX-terminatorconstruct is PCR amplified from a plasmid DNA construct and joined to anautotrophic marker (such as URA3) by either SOE PCR or by commonrestriction digests and cloning. The full cassette, containing thepromoter-coding regionX-terminator-URA3 region, is PCR amplified withprimer sequences that contain 40-70 bp of homology to the regions 5′ and3′ of location “Y” on the yeast chromosome. The PCR product istransformed into yeast and selected on growth media lacking uracil.Transformants can be verified either by colony PCR or by directsequencing of chromosomal DNA.

Any coding regions expressed in the present yeast cells may be codonoptimized for expression in the specific host yeast cell beingengineered as is well known to one skilled in the art. For example, forexpression of the K. lactis and P. stipitis ILV3 coding regions in S.cerevisiae, each was codon optimized for S. cerevisiae expression inExample 1 herein.

Product Biosynthesis with Improved Heterologous Fe—S Protein Activity

Production of any product that has an Fe—S protein contributing to itsbiosynthetic pathway may benefit from the improved activity disclosedherein of a heterologous expressed Fe—S protein in a yeast host withreduced endogenous Fe—S protein expression. For example, DHAD provides astep in pathways for biosynthesis of isobutanol, and RdhtB contributesto a biosynthetic pathway to produce 2-butanone or 2-butanol.

Biosynthetic pathways including a step performed by DHAD for synthesisof isobutanol are disclosed in commonly owned and co-pending US PatentApplication publication US 20070092957 A1, which is herein incorporatedby reference. A diagram of the disclosed isobutanol biosyntheticpathways is provided in FIG. 1. Production of isobutanol in a straindisclosed herein benefits from increased DHAD activity. As described inUS Patent Pub No. US20070092957 A1, steps in an example isobutanolbiosynthetic pathway include conversion of:

-   -   pyruvate to acetolactate (FIG. 1 pathway step a) as catalyzed        for example by acetolactate synthase;    -   acetolactate to 2,3-dihydroxyisovalerate (FIG. 1 pathway step b)        as catalyzed for example by acetohydroxy acid isomeroreductase;    -   2,3-dihydroxyisovalerate to α-ketoisovalerate (FIG. 1 pathway        step c) as catalyzed for example by acetohydroxy acid        dehydratase also called DHAD;    -   α-ketoisovalerate to isobutyraldehyde (FIG. 1 pathway step d) as        catalyzed for example by branched-chain α-keto acid        decarboxylase; and    -   isobutyraldehyde to isobutanol (FIG. 1 pathway step e) as        catalyzed for example by branched-chain alcohol dehydrogenase.

The substrate to product conversions, and enzymes involved in thesereactions, for steps f, g, h, I, j, and k of alternative pathways aredescribed in US 20070092957 A1.

Genes that may be used for expression of the enzymes for the isobutanolpathways are described in US 20070092957 A1, and additional genes thatmay be used can be identified by one skilled in the art throughbioinformatics or experimentally as described above. The preferred usein all three pathways of ketol-acid reductoisomerase (KARI) enzymes withparticularly high activities are disclosed in commonly owned andco-pending US Patent Pub No. US20080261230. Examples of high activityKARIs disclosed therein are those from Vibrio cholerae (DNA: SEQ IDNO:35; protein SEQ ID NO:36), Pseudomonas aeruginosa PAO1, (DNA: SEQ IDNO:37; protein SEQ ID NO:38), and Pseudomonas fluorescens PF5 (DNA: SEQID NO:39; protein SEQ ID NO:40).

Additionally described in US 20070092957 A1 are construction of chimericgenes and genetic engineering of yeast, exemplified by Saccharomycescerevisiae, for isobutanol production using the disclosed biosyntheticpathways.

A biosynthetic pathway including propanediol dehydratase for synthesisof 2-butanone and 2-butanol is disclosed in commonly owned andco-pending US Patent Pub No. US20070292927A1, which is hereinincorporated by reference. A diagram of the disclosed 2-butanone and2-butanol biosynthetic pathway is provided in FIG. 2. 2-Butanone is theproduct made when the last depicted step of converting 2-butanone to2-butanol is omitted. Production of 2-butanone or 2-butanol in a straindisclosed herein benefits from increased coenzyme B12-independentpropanediol dehydratase reactivase activity. As described in US PatentPub No. US20070292927A1, steps in the disclosed biosynthetic pathwayinclude conversion of:

-   -   pyruvate to acetolactate (FIG. 2 step a) as catalyzed for        example by acetolactate synthase;    -   acetolactate to acetoin (FIG. 2 step b) as catalyzed for example        by acetolactate decarboxylase;    -   acetoin to 2,3-butanediol (FIG. 2 step i) as catalyzed for        example by butanediol dehydrogenase;    -   2,3-butanediol to 2-butanone (FIG. 2 step j) as catalyzed for        example by diol dehydratase glycerol dehydratase, or propanediol        dehydratase; and    -   2-butanone to 2-butanol (FIG. 2 step f) as catalyzed for example        by butanol dehydrogenase.

Genes that may be used for expression of these enzymes are described inUS Patent Pub No. US20070292927A1. The use in this pathway in yeast ofthe butanediol dehydratase from Roseburia inulinivorans, RdhtA, (proteinSEQ ID NO:43, coding region SEQ ID NO:15) is disclosed in commonly owedand co-pending US Patent Pub No. US20090155870. This enzyme is used inconjunction with the butanediol dehydratase reactivase from Roseburiainulinivorans, RdhtB, (protein SEQ ID NO:44, coding region SEQ IDNO:16). This butanediol dehydratase is desired in many hosts because itdoes not require coenzyme B₁₂.

Additionally described in US Patent Pub No. US20090155870 areconstruction of chimeric genes and genetic engineering of yeast for2-butanol production using the US 20070292927A1 disclosed biosyntheticpathway.

Fermentation Media

Yeasts disclosed herein may be grown in fermentation media forproduction of a product having an Fe—S protein as part of thebiosynthetic pathway. Fermentation media must contain suitable carbonsubstrates. Suitable substrates may include but are not limited tomonosaccharides such as glucose and fructose, oligosaccharides such aslactose or sucrose, polysaccharides such as starch or cellulose ormixtures thereof and unpurified mixtures from renewable feedstocks suchas cheese whey permeate, cornsteep liquor, sugar beet molasses, andbarley malt. Additionally the carbon substrate may also be one-carbonsubstrates such as carbon dioxide, or methanol for which metabolicconversion into key biochemical intermediates has been demonstrated. Inaddition to one and two carbon substrates methylotrophic organisms arealso known to utilize a number of other carbon containing compounds suchas methylamine, glucosamine and a variety of amino acids for metabolicactivity. For example, methylotrophic yeast are known to utilize thecarbon from methylamine to form trehalose or glycerol (Bellion et al.,Microb. Growth C1 Compd., [Int. Symp.], 7th (1993), 415-32. Editor(s):Murrell, J. Collin; Kelly, Don P. Publisher: Intercept, Andover, UK).Similarly, various species of Candida will metabolize alanine or oleicacid (Sulter et al., Arch. Microbiol. 153:485-489 (1990)). Hence it iscontemplated that the source of carbon utilized in the present inventionmay encompass a wide variety of carbon containing substrates and willonly be limited by the choice of organism.

Although it is contemplated that all of the above mentioned carbonsubstrates and mixtures thereof are suitable in the present invention,preferred carbon substrates are glucose, fructose, and sucrose.

In addition to an appropriate carbon source, fermentation media mustcontain suitable minerals, salts, cofactors, buffers and othercomponents, known to those skilled in the art, suitable for the growthof the cultures and promotion of the enzymatic pathway necessary forproduction of the desired product.

Culture Conditions

Typically cells are grown at a temperature in the range of about 20° C.to about 37° C. in an appropriate medium. Suitable growth media in thepresent invention are common commercially prepared media such as broththat includes yeast nitrogen base, ammonium sulfate, and dextrose as thecarbon/energy source) or YPD Medium, a blend of peptone, yeast extract,and dextrose in optimal proportions for growing most Saccharomycescerevisiae strains. Other defined or synthetic growth media may also beused and the appropriate medium for growth of the particularmicroorganism will be known by one skilled in the art of microbiology orfermentation science.

Suitable pH ranges for the fermentation are between pH 3.0 to pH 7.5,where pH 4.5.0 to pH 6.5 is preferred as the initial condition.

Fermentations may be performed under aerobic or anaerobic conditions,where anaerobic or microaerobic conditions are preferred.

The amount of butanol produced in the fermentation medium can bedetermined using a number of methods known in the art, for example, highperformance liquid chromatography (HPLC) or gas chromatography (GC).

Industrial Batch and Continuous Fermentations

The present process employs a batch method of fermentation. A classicalbatch fermentation is a closed system where the composition of themedium is set at the beginning of the fermentation and not subject toartificial alterations during the fermentation. Thus, at the beginningof the fermentation the medium is inoculated with the desired organismor organisms, and fermentation is permitted to occur without addinganything to the system. Typically, however, a “batch” fermentation isbatch with respect to the addition of carbon source and attempts areoften made at controlling factors such as pH and oxygen concentration.In batch systems the metabolite and biomass compositions of the systemchange constantly up to the time the fermentation is stopped. Withinbatch cultures cells moderate through a static lag phase to a highgrowth log phase and finally to a stationary phase where growth rate isdiminished or halted. If untreated, cells in the stationary phase willeventually die. Cells in log phase generally are responsible for thebulk of production of end product or intermediate.

A variation on the standard batch system is the Fed-Batch system.Fed-Batch fermentation processes are also suitable in the presentinvention and comprise a typical batch system with the exception thatthe substrate is added in increments as the fermentation progresses.Fed-Batch systems are useful when catabolite repression is apt toinhibit the metabolism of the cells and where it is desirable to havelimited amounts of substrate in the media. Measurement of the actualsubstrate concentration in Fed-Batch systems is difficult and istherefore estimated on the basis of the changes of measurable factorssuch as pH, dissolved oxygen and the partial pressure of waste gasessuch as CO₂. Batch and Fed-Batch fermentations are common and well knownin the art and examples may be found in Thomas D. Brock inBiotechnology: A Textbook of Industrial Microbiology, Second Edition(1989) Sinauer Associates, Inc., Sunderland, Mass., or Deshpande, MukundV., Appl. Biochem. Biotechnol., 36:227, (1992), herein incorporated byreference.

Although the present invention is performed in batch mode it iscontemplated that the method would be adaptable to continuousfermentation methods. Continuous fermentation is an open system where adefined fermentation medium is added continuously to a bioreactor and anequal amount of conditioned media is removed simultaneously forprocessing. Continuous fermentation generally maintains the cultures ata constant high density where cells are primarily in log phase growth.

Continuous fermentation allows for the modulation of one factor or anynumber of factors that affect cell growth or end product concentration.For example, one method will maintain a limiting nutrient such as thecarbon source or nitrogen level at a fixed rate and allow all otherparameters to moderate. In other systems a number of factors affectinggrowth can be altered continuously while the cell concentration,measured by media turbidity, is kept constant. Continuous systems striveto maintain steady state growth conditions and thus the cell loss due tothe medium being drawn off must be balanced against the cell growth ratein the fermentation. Methods of modulating nutrients and growth factorsfor continuous fermentation processes as well as techniques formaximizing the rate of product formation are well known in the art ofindustrial microbiology and a variety of methods are detailed by Brock,supra.

The present invention may be practiced using either batch, fed-batch orcontinuous processes and known modes of fermentation are suitable.Additionally, it is contemplated that cells may be immobilized on asubstrate as whole cell catalysts and subjected to fermentationconditions for 1-butanol production.

Methods for Butanol Isolation from the Fermentation Medium

Bioproduced butanol may be isolated from the fermentation medium usingmethods known in the art. For example, solids may be removed from thefermentation medium by centrifugation, filtration, decantation, or thelike. Then, the butanol may be isolated from the fermentation medium,which has been treated to remove solids as described above, usingmethods such as distillation, liquid-liquid extraction, ormembrane-based separation. Because butanol forms a low boiling point,azeotropic mixture with water, distillation can only be used to separatethe mixture up to its azeotropic composition. Distillation may be usedin combination with another separation method to obtain separationaround the azeotrope. Methods that may be used in combination withdistillation to isolate and purify butanol include, but are not limitedto, decantation, liquid-liquid extraction, adsorption, andmembrane-based techniques. Additionally, butanol may be isolated usingazeotropic distillation using an entrainer (see for example Doherty andMalone, Conceptual Design of Distillation Systems, McGraw Hill, NewYork, 2001).

The butanol-water mixture forms a heterogeneous azeotrope so thatdistillation may be used in combination with decantation to isolate andpurify the butanol. In this method, the butanol containing fermentationbroth is distilled to near the azeotropic composition. Then, theazeotropic mixture is condensed, and the butanol is separated from thefermentation medium by decantation. The decanted aqueous phase may bereturned to the first distillation column as reflux. The butanol-richdecanted organic phase may be further purified by distillation in asecond distillation column.

The butanol may also be isolated from the fermentation medium usingliquid-liquid extraction in combination with distillation. In thismethod, the butanol is extracted from the fermentation broth usingliquid-liquid extraction with a suitable solvent. The butanol-containingorganic phase is then distilled to separate the butanol from thesolvent.

Distillation in combination with adsorption may also be used to isolatebutanol from the fermentation medium. In this method, the fermentationbroth containing the butanol is distilled to near the azeotropiccomposition and then the remaining water is removed by use of anadsorbent, such as molecular sieves (Aden et al. Lignocellulosic Biomassto Ethanol Process Design and Economics Utilizing Co-Current Dilute AcidPrehydrolysis and Enzymatic Hydrolysis for Corn Stover, ReportNREL/TP-510-32438, National Renewable Energy Laboratory, June 2002).

Additionally, distillation in combination with pervaporation may be usedto isolate and purify the butanol from the fermentation medium. In thismethod, the fermentation broth containing the butanol is distilled tonear the azeotropic composition, and then the remaining water is removedby pervaporation through a hydrophilic membrane (Guo et al., J. Membr.Sci. 245, 199-210 (2004)).

EXAMPLES

The present invention is further defined in the following Examples. Itshould be understood that these Examples, while indicating preferredembodiments of the invention, are given by way of illustration only.From the above discussion and these Examples, one skilled in the art canascertain the essential characteristics of this invention, and withoutdeparting from the spirit and scope thereof, can make various changesand modifications of the invention to adapt it to various uses andconditions.

General Methods

Standard recombinant DNA and molecular cloning techniques used in theExamples are well known in the art and are described by Sambrook, J.,Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual;Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y. (1989)(Maniatis) and by T. J. Silhavy, M. L. Bennan, and L. W. Enquist,Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, ColdSpring Harbor, N.Y. (1984) and by Ausubel, F. M. et al., CurrentProtocols in Molecular Biology, pub. by Greene Publishing Assoc. andWiley-Interscience (1987), and by Methods in Yeast Genetics, 2005, ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

Materials and methods suitable for the maintenance and growth ofbacterial cultures are well known in the art. Techniques suitable foruse in the following Examples may be found as set out in Manual ofMethods for General Bacteriology (Phillipp Gerhardt, R. G. E. Murray,Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg andG. Briggs Phillips, eds), American Society for Microbiology, Washington,D.C. (1994)) or by Thomas D. Brock in Biotechnology: A Textbook ofIndustrial Microbiology, Second Edition, Sinauer Associates, Inc.,Sunderland, Mass. (1989). All reagents, restriction enzymes andmaterials used for the growth and maintenance of microbial cells wereobtained from Aldrich Chemicals (Milwaukee, Wis.), BD Diagnostic Systems(Sparks, Md.), Life Technologies (Rockville, Md.), or Sigma ChemicalCompany (St. Louis, Mo.) unless otherwise specified. Microbial strainswere obtained from The American Type Culture Collection (ATCC),Manassas, Va., unless otherwise noted. All the oligonucleotide primerswere synthesized by Sigma-Genosys (Woodlands, Tex.) or Integrated DNATechnologies (Coralsville, Iowa).

Synthetic complete medium is described in Amberg, Burke and Strathern,2005, Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press,Cold Spring Harbor, N.Y.

HPLC

Analysis for fermentation by-product composition is well known to thoseskilled in the art. For example, one high performance liquidchromatography (HPLC) method utilizes a SHODEX SH-1011 column with aSHODEX SH-G guard column (both available from Waters Corporation,Milford, Mass.), with refractive index (RI) detection. Chromatographicseparation is achieved using 0.01 M H₂SO₄ as the mobile phase with aflow rate of 0.5 mL/min and a column temperature of 50° C. Isobutanolretention time is 47.6 minutes.

The meaning of abbreviations is as follows: “s” means second(s), “min”means minute(s), “h” means hour(s), “psi” means pounds per square inch,“nm” means nanometers, “d” means day(s), “μL” means microliter(s), “mL”means milliliter(s), “L” means liter(s), “mm” means millimeter(s), “nm”means nanometers, “mM” means millimolar, “M” means molar, “mmol” meansmillimole(s), “μmol” means micromole(s)“, “g” means gram(s), “μg” meansmicrogram(s) and “ng” means nanogram(s), “PCR” means polymerase chainreaction, “OD” means optical density, “OD₆₀₀” means the optical densitymeasured at a wavelength of 600 nm, “kDa” means kilodaltons, “g” meansthe gravitation constant, “bp” means base pair(s), “kbp” means kilobasepair(s), “% w/v” means weight/volume percent, % v/v” means volume/volumepercent, “wt %” means percent by weight, “HPLC” means high performanceliquid chromatography, and “GC” means gas chromatography. The term“molar selectivity” is the number of moles of product produced per moleof sugar substrate consumed and is reported as a percent.

Example 1 Expression of DHAD from K. lactis in LEU1 Deletion Strain ofS. cerevisiae

The yeast LEU1 gene encodes isopropylmalate dehydratase, an enzyme thatrequires an Fe—S cluster for its function. The impact of LEU1 deletionon DHAD activity expressed from the Kluyveromyces lactis DHAD codingregion was examined in this example. For gene expression in yeast, theshuttle vector pNY13 (SEQ ID NO:29) derived from pRS423 was used. Thisshuttle vector contained an F1 origin of replication (1423 to 1879) formaintenance in E. coli and a 2 micron origin (nt 7537 to 8881) forreplication in yeast. The vector has an FBA promoter (nt 2111 to 3110)and FBA terminator (nt 4316 to 5315). In addition, it carries the HIS3marker (nt 504 to 1163) for selection in yeast and ampicillin resistancemarker (nt 6547 to 7404) for selection in E. coli. pNY9 is the samevector with a URA3 marker replacing the HIS3 marker.

The ILV3 coding region for DHAD from Kluyveromyces lactis wassynthesized with codon-optimization for expression in S. cerevisiae byDNA 2.0 (Menlo Park, Calif.). The cloned synthesized sequence was PCRamplified. During amplification, a portion of the mitochondrial signalpeptide for the DHAD at the N-terminus was deleted by usingilv3(K)(0)-F(delet) as the forward primer with ilv3(K)(o)-R as thereverse primer, resulting in a coding region for cytoplasmic expression(SEQ ID NO:173). In addition, an SphI site was incorporated in theforward primer, while a NotI site was included in the reverse primer.The PCR product was cloned into the shuttle vectors pNY9 and pNY13 sothat the ILV3 coding region was under the control of the FBA promoter.Both PCR product and each vector (pNY9, pNY13) were digested with SphIand NotI. After digestion, the components were ligated, and the ligationmixture was transformed into TOP10 competent cells (Invitrogen).Transformants were selected in LB agar plates supplemented with 100μg/ml of ampicillin. Positive clones were screened by PCR with theforward and reverse primers described above. The resulting plasmids weredesignated as pRS423::FBAp-ILV3(KL) and pRS426::FBAp-ILV3(KL), derivedfrom plasmids pNY13 and pNY9, respectively.

To study the expression of the DHAD from K. lactis in S. cerevisiae theexpression vector pRS423::FBAp-ILV3(KL) along with an empty vectorpRS426 were transformed into strains BY4743 and BY4743 leu1:kanMX4 (ATCC4034377). The competent cell preparation and transformation were basedon the Frozen Yeast Transformation kit from Zymo Research. Thetransformants were selected on agar plates with yeast synthetic mediumlacking histidine and uracil (Teknova). For enzymatic assays, thestrains carrying the expression construct and the empty vector pRS426were first grown overnight in 5 ml synthetic complete yeast mediumlacking histidine and uracil. The 5 ml overnight cultures weretransferred into 100 ml of medium in a 250 ml flask. The cultures wereharvested when they reached 1 to 2 O.D. at 600 nm. The samples werewashed with 10 ml of 20 mM Tris (pH 7.5) and then resuspended in 1 ml ofthe same Tris buffer. The samples were transferred into 2.0 ml tubescontaining 0.1 mm silica (Lysing Matrix B, MP biomedicals). The cellswere then broken in a bead-beater (BIO101). The supernatant was obtainedby centrifugation in a microfuge at 13,000 rpm at 4° C. for 30 minutes.Typically, 0.06 to 0.1 mg of crude extract protein was used in a DHADassay. Protein in the crude extracts was determined by Bradford assaywith Coomassie stain.

Dihydroxy-Acid Dehydratase Enzyme Assay

The in vitro DHAD enzyme assay is a variation on the assay described inFlint et al. (J. Biol. Chem. (1993) 268:14732-14742.). The assay wasperformed in a 1.6 ml total volume and consisted of: 800 μl 2× buffer(100 mM Tris pH 8.0, 20 mM MgCl₂), 160 μl 10× substrate (15.6 mg/mldihydroxyisovalerate), crude extract (typically 50-200 μg protein), andwater. The reaction was incubated at 37° C. At 0, 30, 60, and 90 minutetime intervals, 350 μl aliquots of the reaction were removed andincubated with 350 μl of 0.05% dinitrophenylhydrazine in 1N HCl for 30minutes at 25° C. To quench the reaction, 350 μl of 4N sodium hydroxidewas added to the reaction mixture, and the reaction was centrifuged at15,000×g for 2 minutes. The supernatant was transferred to a plasticdisposable cuvette and absorbance at 540 nm was measured in aspectrophotometer. The amount of α-ketoisovalerate (KIV) produced wasdetermined by entering the absorbance into the linear regressionequation obtained from a standard curve of α-ketoisovalerate. The amountof KIV produced at each time point was plotted to determine the rate ofproduction. The slope of the linear regression was then used tocalculate specific activity using the formula:Specific activity calculation=(slope of KIV production/1000)/mg proteinper 1.6 mL reaction=mmol/min*mg

The dehydratase from K. lactis had a specific activity in the range of0.2 to 0.35 μmol min⁻¹ mg⁻¹ when expressed in yeast strain BY4743(Δleu1). In contrast, this enzyme had a specific activity in the rangeof only 0.14 μmol min⁻¹ mg⁻¹ when expressed in the parent yeast strainBY4743. Strains BY4743 (Δleu1) and wildtype BY4743 containing emptyvectors pRS423 or pRS426 had a background of activity in the range of0.03 to 0.1 μmol min⁻¹ mg⁻¹.

Example 2 Expression of Diol Dehydratase in LEU1 Deletion Strain of S.cerevisiae

A coenzyme B12-independent propanediol dehydratase is disclosed incommonly owned and co-pending US Patent Pub No. US20090155870. Thesequences encoding this coenzyme B12-independent (S-adenosylmethionine(SAM)-dependent) propanediol dehydratase (SEQ ID NO:15) and its putativeassociated reactivase (SEQ ID NO:16) in the bacterium Roseburiainulinivorans [Scott et al. (2006) J. Bacteriol. 188:4340-9], hereafterreferred to as rdhtA and rdhtB, respectively, were synthesized as oneDNA fragment (SEQ ID NO:17) by standard methods and cloned into an E.coli vector (by DNA2.0, Inc., Menlo Park, Calif.). This clone was usedas a PCR template to prepare separate RdhtA and RdhtB coding regionfragments. The RdhtA coding region for the diol dehydratase wasamplified by PCR using primers N695 and N696 (SEQ ID NOs:18 and 19). TheRdhtB coding region for the diol dehydratase activase, was amplified byPCR using primers N697 and N698 (SEQ ID NOs:20 and 21). The two DNAfragments were combined with a dual terminator DNA fragment (SEQ IDNO:22) that has an ADH terminator (SEQ ID NO:23) and a CYC1 terminator(SEQ ID NO:24) adjacent to each other in opposing orientation using SOEPCR (Horton et al. (1989) Gene 77:61-68). The dual terminator fragmentwas isolated as a 0.6 kb fragment following PacI digestion ofpRS426::FBA-ILV5+GPM-kivD (described in co-owned and co-pending USPatent Publication 20070092957 A1, Example 17). The resulting 4 kb DNAfragment had the RdhtA and RdhtB coding regions in opposing orientationon either side of the dual terminator, with the 3′end of each codingregion adjacent to the dual terminator sequence. This DNA fragment wasthen cloned by gap repair methodology (Ma et al. (1987) Genetics58:201-216) into the S. cerevisiae shuttle vectorpRS426::FBA-ILV5+GPM-kivD that was prepared by digestion with BbvCl toremove the ILV5 and kivD coding regions and dual terminator sequencebetween their 3′ ends. The resulting plasmid, pRS426::RdhtAB (below),contained the RdhtA gene under the control of the S. cerevisiae FBApromoter (SEQ ID NO:25) and the RdhtB gene under control of the S.cerevisiae GPM promoter (SEQ ID NO:26).

Plasmids pRS426 and pRS426::RdhtAB were introduced into S. cerevisiaestrains BY4743 (ATCC 201390) and BY4743 leu1:kanMX4 (ATCC 4034377) bystandard techniques (Methods in Yeast Genetics, 2005, Cold Spring HarborLaboratory Press, Cold Spring Harbor, N.Y., pp. 201-202). Cells wereplated on synthetic complete medium lacking uracil to select fortransformants. Transformants were tested for diol dehydratase activityusing an in vivo assay as follows. Patched cells grown on solid mediumwere used to inoculate liquid media (20 ml) in petri plates. Media usedwere synthetic complete minus uracil with and without addition of 5 g/L1,2-propanediol 9Aldrich Cat. No. 398039). The petri plates weretransferred to an Anaeropack™ System jar (Mitsubishi Gas Chemical Co.Cat. No. 50-70). An anaerobic environment (<0.1% oxygen) was generatedusing PACK-ANAERO sachets (Mitsubishi Gas Chemical Co. Cat. No. 10-01).After 48 hours, culture supernatants were sampled, filtered and analyzedby HPLC as described in General Methods. Propanol, which has a retentiontime of 38.8 minutes, was observed in culture supernatants of strainscarrying pRS426::RdhtAB when 1,2-propanediol was provided in the medium.The results given in Table 5 show that more propanol was produced in thesupernatants of the strain also carrying the LEU1 deletion than in thestrain without the LEU1 deletion. Statistical analysis gave a P score ofless than 0.0005.

TABLE 5 Propanol production with propanediol dehydratase/reactivaseexpression in yeast with and without LEU1 knockout. 1,2-propanediolPropanol Peak Strain Added Area BY4743 5 g/L 19472 ± 1403Δleu1::kanMX4/pRS426::RdhtAB (n = 6) BY4743 0 g/L 2478 (n = 1)Δleu1::kanMX4/pRS426::RdhtAB BY4743/pRS426::RdhtAB 5 g/L 11830 ± 1963 (n= 6) BY4743/pRS426::RdhtAB 0 g/L 2369 (n = 1) BY4743Δleu1::kanMX4/pRS426 5 g/L 2633 (n = 1) BY4743/pRS426 5 g/L 2841 (n = 1)

Example 3 Improving Cytosolic Dihydroxy-Acid Dehydratase (DHAD) Activityin S. cerevisiae Through a Disruption of Mitochondrial ILV3

Vector/Host Construction

In S. cerevisiae ILV3 encodes the mitochondrial dihydroxy-aciddehydratase that is involved in branched chain amino acid biosynthesis.To reduce background from endogenous ILV3 expression for in vitroenzymatic assays in S. cerevisiae, an ilv3::URA3 disruption cassette wasconstructed by PCR amplification of the URA3 marker from pRS426 (ATCCNo. 77107) with primers “ILV3::URA3 F” and “ILV3::URA3 R”, given as SEQID NO:30 and 31. These primers produced a 1.4 kb URA3 PCR product thatcontained 70 bp 5′ and 3′ extensions identical to sequences upstream anddownstream of the ILV3 chromosomal locus for homologous recombination.The PCR product was transformed into BY4741 cells (ATCC 201388) usingstandard genetic techniques (Methods in Yeast Genetics, 2005, ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202)and resulting transformants were maintained on synthetic complete medialacking uracil and supplemented with 2% glucose at 30° C. Transformantswere screened by PCR using primers “ILV3 F Check” and “URA3 REV Check”,given as SEQ ID NOs:32 and 33, to verify integration at the correct siteand disruption of the endogenous ILV3 locus. The correct transformantshad the genotype: BY4741 ilv3::URA3.

Construction of plasmid pRS423::FBAp-ILV3(KL) and pRS426::FBAp-ILV3(KL)were described in Example 1. Construction of pRS423::CUP1-alsS+FBA-ILV3has been described in co-owned and co-pending US Patent PublicationUS20070092957 A1, Example 17 which is herein incorporated by reference.pRS423::CUP1-alsS+FBA-ILV3 is the same plasmid aspRS423::CUP1p-alsS-FBAp-ILV3. This construction contains a chimeric genecontaining the S. cerevisiae CUP1 promoter (SEQ ID NO:34), alsS codingregion from Bacillus subtilis (SEQ ID NO:27), and CYC1 terminator (SEQID NO:24); and also a chimeric gene containing the S. cerevisiae FBApromoter (SEQ ID NO:25), ILV3 coding region from S. cerevisiae lackingthe mitochondrial targeting signal coding sequence (SEQ ID NO:111) andADH1 terminator (SEQ ID NO:23).

Preparation of Samples

Plasmid vectors pRS423::CUP1p-alsS-FBAp-/LV3 and pRS423::FBAp-ILV3(KL)were transformed into strain BY4741 ilv3::URA3 using standard genetictechniques (Methods in Yeast Genetics, 2005, Cold Spring HarborLaboratory Press, Cold Spring Harbor, N.Y.) and maintained on syntheticcomplete media lacking histidine. Plasmid vectorspRS423::CUP1p-alsS-FBAp-/LV3 and pRS426::FBAp-ILV3(KL) were alsotransformed into strain BY4741. Aerobic cultures were grown in 1000 mlflasks containing 200 ml synthetic complete media lacking histidine andsupplemented with 2% glucose in an INNOVA 4000 incubator (New BrunswickScientific, Edison, N.J.) at 30° C. and 225 rpm. Cultures were harvestedat OD600 measurements of 1.0-2.0 and pelleted by centrifugation at6000×g for 10 minutes. Cell pellets were washed with 10 mM Tris-HCl, pH8.0 and pellets were stored at −80° C. until assayed for activity. Cellfree extracts were prepared by standard bead beating method using 1 mlof 0.5 mm beads and 1.5 ml of yeast cell suspension. Proteinconcentration in the extracts was determined by Bradford assay withCoomassie stain. DHAD enzyme assays and specific activity calculationswere performed as described in Example 1. The results given in Table 6show that there was higher DHAD activity in the ILV3 deletion cells thanin cells without the ILV3 deletion.

TABLE 6 DHAD activity in yeast with and without ILV3 deletion. AverageSpecific Activity Specific Activity Strain (μmol/min*mg) (μmol/min*mg)BY4741 0.018 0.013 0.014 0.008 BY4741 pRS423::CUP1p-alsS-FBAp-ILV3 0.0180.019 0.020 BY4741 pRS426::FBAp-ILV3(KL) 0.040 0.038 0.036 BY4741ilv3::URA3 0.00006 0.00041 0.00075 BY4741 ilv3::URA3 pRS423::CUP1p-alsS-0.030 0.029 FBAp-ILV3 0.028 BY4741 ilv3::URA3 pRS423::FBAp-ILV3(KL)0.317 0.281 0.244Verification of Alpha-Ketoisovalerate Formation by HPLC

Formation of alpha-ketoisovalerate from the in vitro DHAD enzyme assayswas accomplished using HPLC and semicarbazide derivatization. DHADenzyme assays were performed in a 1.6 ml total volume and consisted of:800 μl 2× buffer (100 mM Tris pH 8.0, 20 mM MgCl₂), 160 μl 10× substrate(15.6 mg/ml dihydroxyisovalerate), crude extract (typically 50-200 μgprotein), and water. The reactions were incubated at 37° C. At timeintervals of zero and 90 minutes 350 μl aliquots of the reactions wereremoved, transferred to ice, and centrifuged at 13,000×g for 2 minutesat 4° C. to remove precipitated protein. The supernatants weretransferred to ice-chilled MICROCON YM-10 (Sigma) spin columns andcentrifuged at 13,000×g for 20 minutes at 4° C. to remove enzymes andsoluble proteins. The flowthroughs were mixed with 100 μl derivatizingreagent (1% semicarbazide hydrochloride and 1.5% sodium acetatetrihydrate) and incubated at room temperature for 15 minutes. Thereactions were spun through COSTAR spin filters (CoStar, 0.22 μm filter)at 13,000×g for 5 minutes at 4° C. to remove any precipitates. Theflowthroughs were transferred to HPLC vials for analysis.

Analysis of derivatized alpha-ketoisovalerate was conduced using reversephase chromatography on a SUPELCOSIL LC-18 column with SUPELGUARDLC-18-DB guard column (Supelco; 25 cm×4.6 mm, 5 μm). Injection volumeswere 10 μl. Mobile phases were methanol (A) and 50 mM NaOAc pH 7.2. Thegradient program utilized is given in Table 7, with detection at 250 nm.

TABLE 7 Gradient used for derivatized alpha-ketoisovalerate HPLC assayTime (min) Flow (ml/min) % NaOAc (50 mM) % MEOH Curve Initial 1.0 95 5 51.0 95 5 6 20 1.0 70 30 6 21 1.0 0 100 6 25 1.0 0 100 6 26 1.0 95 5 6 351.0 95 5 6 The retention time of semicarbizide-derivatizedalpha-ketoisovalerate was 11.5 minutes.

The results, which are given in Table 8, confirmed that KIV was producedin the cells, as detected in the indirect assay for specific activityabove. The amount of KIV listed in the DHAD Assay column is the amountdetermined indirectly in the 90 min sample for the activity assaydescribed above in determining the specific activity. This amount of KIVcorrelates well with the amount detected in the HPLC assay.

TABLE 8 Comparison of KIV detected in DHAD activity assay and by HPLC.keto-isovalerate production (μM) Strain DHAD Assay HPLC BY4741ilv3::URA3 pRS423::FBAp-ILV3(KL) 256 256 BY4741 ilv3::URA3pRS423::FBAp-ILV3(KL) 162 163

TABLE 9 A C D E F G H I K Position in m -> m -> m -> i -> i -> d -> d ->b -> m -> HMM m i d m i m d m e L M N P Q R S T V W Y alignment −538 *−1684  1 (M) −233 −1296 99 1223 −1477 −1132 89 −1122 420 −1248 1757 1553−1296 464 −24 −190 −188 −838 −1578 −985 6 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −29 −6203−7245 −894 −1115 −701 −1378 −538 *  2 (E) −220 −1288 232 1356 −1807 1016−70 −1474 190 −1584 −775 132 −1298 300 −282 −183 1140 −1092 −1872 −12627 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −29 −6203 −7245 −894 −1115 −701 −1378 * *  3 (K)−448 −1932 1558 658 −2220 −1048 40 −1983 1569 −1938 −1091 1558 −1319 450−193 −278 −419 −1552 −2121 −1397 8 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −29 −6203 −7245−894 −1115 −701 −1378 * *  4 (V) −404 −498 −1497 −939 −588 −1810 −6401591 914 −127 335 −962 −1866 −562 −767 −868 −357 1720 −1169 −763 9 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −29 −6203 −7245 −894 −1115 −701 −1378 * *  5 (E) −265−1340 −52 1376 −1572 −1189 113 −1125 1345 −1287 −496 99 −1321 505 198−218 −205 597 −1598 −1032 10 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −29 −6203 −7245 −894−1115 −701 −1378 * *  6 (S) 256 −397 −1014 −830 −1841 −646 −862 −1443−767 −1740 −963 −568 −1249 −651 −1007 2267 1586 −862 −2080 −1672 11 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −29 −6203 −7245 −894 −1115 −701 −1378 * *  7 (M) −990−889 −2630 157 −513 −2514 −1346 1309 −1767 820 3683 −1898 −2491 −1496−1799 −1589 −925 150 −1336 −1041 12 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  8 (E) 588 −1875 −194 1536 −2188 −1373 −59−1931 957 −1890 −977 904 292 393 −162 483 −372 −1495 −2070 −1391 13 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  9 (N) −514−1116 1207 −315 447 −1650 −304 −778 −224 825 −277 1457 −1738 −123 −618−627 −454 −603 −1186 763 14 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * *  10 (N) −815 −1190 −1360 −922 −904 −1967 −797 −442−670 381 1700 3009 −2099 −654 −934 −1051 −791 −445 −1490 −979 15 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  11 (K) −1530−2498 −1722 −855 −3141 −2246 −428 −2627 2828 −2404 −1656 −927 662 −22047 −1421 −1337 −2324 −2357 −2081 16 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  12 (Y) −872 −1887 −861 −290 −1369 −18011662 −1797 325 −1793 −1031 893 −1876 56 2219 −812 −780 −1514 −1565 228717 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  13 (S)−830 −1586 −1471 −1099 −2717 −1642 −1010 −2479 −266 −2518 −1746 −1065−2069 −676 1822 2748 −1000 −1950 −2597 −2189 18 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * *  14 (Q) −851 −2131 −775 −153 −2554−1735 −211 −2205 1908 −2094 −1244 −386 −1802 2254 974 1001 −747 −1819−2181 −1667 19 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  15 (T) −405 −1258 −618 −100 −1490 −1466 1158 −1121 1 −1299−514 578 −1607 65 −433 960 1849 343 −1677 −1143 20 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * *  16 (I) −1772 −1325 −4307−3877 −1405 −3993 −3383 2935 −3705 820 −217 −3632 −3761 −3400 −3682−3260 −1742 2033 −2838 −2525 21 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * *  17 (T) −1018 −1329 −2004 −1771 −409 −1993 −1000−1256 −1512 −1464 −966 −1543 −2367 −1428 −1638 −1257 3050 −1090 −10122448 22 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  18(Q) −1509 −3056 1970 44 −3310 −1666 −896 −3242 −877 −3158 −2439 −322−2123 3562 −1493 −1259 −1550 −2779 −3260 −2446 23 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * *  19 (D) −1006 −2199 2178 −88−3159 1997 −936 −2974 −948 −2977 −2174 −382 −1960 −589 −1571 1295 −1157−2369 −3178 −2430 24 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  20 (M) 445 −796 −1082 −521 −841 −1643 −412 −403 −370 −6922213 −646 536 1166 −698 −630 660 831 −1204 −767 25 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * *  21 (Q) 741 −990 −1025 −507−1249 −1551 −519 −720 −357 −1062 −345 −635 −1739 1770 −713 −589 15761129 −1559 −1097 26 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  22 (R) −1753 −2648 −2072 −1047 −3365 −2405 −452 −2782 1989−2495 −1773 −1062 −2379 2402 2643 −1629 −1506 −2504 −2397 −2190 27 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  23 (S) −330−1010 −1820 −1628 −2778 −1229 −1652 −2481 −1592 −2691 −1841 −1273 2130−1426 −1834 2449 1034 −1716 −2961 −2594 28 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  24 (P) 1882 −1119 −2231 −2302 −3062−1360 −2209 −2710 −2339 −3013 −2243 −1676 3304 −2117 −2409 −742 −918−1916 −3263 −3022 29 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  25 (N) 969 −1230 −1066 −915 −2593 −1313 −1196 −2242 −1033−2447 −1626 3197 −1850 −898 −1392 −582 1155 −1644 −2736 −2256 30 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  26 (R) −1847−2640 −2014 −1161 −3282 −2428 −579 −2818 687 −2553 −1869 −1165 −24622447 3181 −1746 −1630 −2555 −2447 −2228 31 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  27 (A) 3048 −932 −2480 −2533 −3075−1200 −2274 −2765 −2501 −3071 −2221 −1658 −1948 −2205 −2512 1225 −739−1842 −3322 −3078 32 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  28 (M) −2406 −2296 −3638 −3594 −1525 −3105 −2824 −1047 −3121−596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398 −2513 −2207 33 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  29 (Y) −1674−1506 −2863 −2464 596 −2872 2251 −972 −2024 2197 −552 −1986 −2876 −1739−1988 −1987 −1601 −1002 −95 2332 34 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  30 (Y) −2013 −2305 −2428 −1781 −328 −2709−654 −2240 −258 −2064 −1626 −1631 −2788 −899 2789 −2017 −1896 −2130 −8573434 35 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  31(A) 2822 −1031 −2418 −2539 −3226 1898 −2364 −2941 −2626 −3229 −2379−1722 −2026 −2302 −2634 −654 −848 −1983 −3415 −3226 36 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * *  32 (I) −1247 −941 −3569−3039 −1082 −3101 −2185 2227 −2763 766 −76 −2700 −3050 −2469 −2697 −22531322 1974 −1988 −1633 37 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  33 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761−3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −422238 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  34 (F)−1511 −1236 −3511 −3017 2747 −2982 −1069 −260 −2651 992 2737 −2407 −2904−2088 −2418 −2099 −1434 −489 −537 2056 39 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  35 (Q) −576 −1869 −401 92 −2232 831−173 −1930 1505 −1913 −1042 −186 −1620 1653 −51 −482 1346 −1534 −2098−1490 40 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 36 (D) −1352 −3066 3028 1349 −3303 −1566 −724 −3141 1155 −3043 −2267−165 −1991 −354 −1350 −1086 −1368 −2659 −3221 −2356 41 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * *  37 (E) −1507 −3288 20422762 −3520 515 −853 −3401 −981 −3296 −2566 −182 −2064 −503 −1753 −1209−1553 −2895 −3486 −2547 42 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  38 (D) −1445 −2778 3529 −53 −3524 −1590 −1129 −3476−1367 −3459 −2774 −396 −2156 −825 −2122 554 −1609 −2880 −3582 −2717 43 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  39 (F) −2658−2176 −4213 −4000 3815 −3933 −1352 −531 −3638 1121 −19 −3184 −3709 −2820−3296 −3219 −2579 −1037 −601 403 44 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  40 (D) −684 −2193 1738 1460 −2494 −1437 −249−2257 1694 −2199 −1308 −62 −1637 185 −450 −531 633 −1808 −2374 −1657 45— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  41 (K) −2620−2961 −2461 −2046 −3743 −2791 −1570 −3603 3784 −3387 −2839 −2048 −3039−1260 −465 −2604 −2536 −3331 −3001 −2988 46 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  42 (P) 1882 −1119 −2231 −2302 −3062−1360 −2209 −2710 −2339 −3013 −2243 −1676 3304 −2117 −2409 −742 −918−1916 −3263 −3022 47 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  43 (I) −1006 −992 −2347 −1784 −650 −2452 −1256 2372 −1386 772213 −1720 −2455 2030 −1490 −1528 −946 106 −1441 −1111 48 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  44 (V) −1771 −1603−3750 −3689 −2037 −3050 −3231 403 −3479 −1154 −1076 −3246 −3399 −3383−3437 −2628 −1917 3536 −3074 −2677 49 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  45 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 50 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  46 (I) −1759 −1303 −4330 −3968 −1751 −4051 −3743 3027 −3837−597 −528 −3729 −3875 −3688 −3910 −3369 −1751 2438 −3259 −2819 51 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  47 (V) 1736 −1012−3546 −3078 −1377 −3073 −2434 2052 −2843 −608 −331 −2754 −3122 −2619−2855 −2270 −1277 2193 −2333 −1941 52 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  48 (N) −686 −1511 −702 −806 −2927 −1386−1339 −2841 −1264 −2950 −2137 2702 −1979 −1062 −1648 2444 −971 −2105−3054 −2475 53 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  49 (M) −411 −857 −1800 −1434 −1528 1914 −1202 −1029 −1247−1347 2989 −1217 −1912 −1119 −1444 −676 1550 −767 −1922 −1539 54 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  50 (W) −782 −1258793 −683 1193 346 2051 −932 −556 −1092 −441 −798 −1993 −426 −909 −904−720 −779 3163 1546 55 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  51 (W) 1009 −798 −1470 −935 −463 −1773 −545 −460 −751−736 −66 −943 −1904 −606 −1002 1604 −507 −322 2535 1521 56 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  52 (D) −1137 −27112125 1647 −2995 −1523 −617 −2786 −528 −2743 −1933 −150 −1897 −234 −1165−924 2117 −2331 −2948 −2141 57 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * *  53 (I) −599 −1102 −1031 −829 −1522 1429 −927 2119−880 −1369 −699 1692 −1938 −759 −1188 −799 −698 −689 −1887 −1419 58 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  54 (T) −666−1412 −954 −984 −2702 −1428 −1357 −2418 −1208 −2650 −1886 2293 −2000−1101 −1519 −787 2967 −1835 −2866 −2360 59 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  55 (P) −632 −1230 −2074 −2144 −2996−1453 −2116 −2631 −2128 −2928 −2213 −1658 3610 −2006 −2221 −852 1302−1931 −3185 −2917 60 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  56 (C) −2476 5735 −4102 −4358 −3712 −2763 −3545 −3518 −4167−3859 −3569 −3631 −3363 −4030 −3832 −2793 −2860 −3158 −3464 −3718 61 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  57 (N) −2171−2655 −1458 −1748 −3334 −2364 −2267 −3943 −2365 −3936 −3437 4205 −2932−2205 −2608 −2224 −2439 −3392 −3253 −2909 62 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  58 (M) 672 −918 −3119 −2578 −742 −2668−1734 1807 −2263 16 3713 −2271 −2704 −1960 −2216 −1806 −1058 493 −1612−1306 63 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 1378 * *  59(H) −1525 −2164 −1235 −1346 −2509 2296 4235 −3172 −1516 −3178 −2523−1448 −2541 −1520 −1760 −1591 −1741 −2656 −2681 −2065 64 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * *  60 (L) −2478 −2009 −4717−4196 −568 −4424 −3262 1334 −3887 2824 604 −4085 −3872 −3088 −3590 −3717−2380 −199 −2217 −2207 65 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  61 (H) −682 −2191 1015 275 −2485 396 2379 −2251 62 −2197−1307 1826 −1636 1527 −480 −529 −641 −1803 −2375 −1654 66 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  62 (D) −575 −1920 1979184 −2299 94 −242 −2029 114 −2023 −1144 −120 −1608 186 1063 −469 1413−1605 −2229 −1561 67 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  63 (L) −2618 −2139 −4597 −4163 2144 −4285 −2334 −83 −38542690 538 −3771 −3806 −2950 −3488 −3563 −2505 −751 −1442 −808 68 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  64 (A) 2657 −1033−2408 −2532 −3233 2193 −2364 −2950 −2626 −3237 −2386 −1719 −2027 −2301−2635 −655 −850 −1988 −3420 −3231 69 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  65 (K) −443 −1857 958 270 −2158 −1393−66 −1890 1839 −442 −957 −36 −1499 1204 −132 616 −382 −1469 −2048 −138370 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  66 (C)605 1553 739 −17 −1374 −1488 −182 260 969 −203 −397 −263 −1573 159 691−426 −331 −761 −1567 −1032 71 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * *  67 (A) 2327 −956 −3193 −2728 −1289 −2677 −21141664 −2485 −601 −288 −2403 −2839 −2263 −2523 −1871 −1126 1617 −2143−1765 72 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 68 (K) −532 −1656 −490 1321 −1891 −1527 −172 −124 2206 −1591 −782 −223−1619 237 −106 −482 −464 −98 −1904 −1326 73 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  69 (H) 384 −1854 936 889 −2165 −13631498 −1909 1111 −1866 −948 1091 −1464 421 −131 −284 −342 −69 −2043 −136474 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  70 (G)1823 −932 −2330 −2313 −3120 2511 −2158 −2865 −2331 −3098 −2209 −1563−1912 −2032 −2419 1138 −706 −1883 −3328 −3077 75 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * *  71 (V) −1760 −1333 −4244 −3789−1262 −3902 −3190 1495 −3588 1270 −96 −3536 −3677 −3238 −3534 −3148−1725 2865 −2654 −2373 76 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  72 (W) −1054 −2172 −1112 −403 −2566 −1917 −286 −21962516 −2095 −1292 1183 −1958 140 1333 −959 −922 −1867 2591 −1720 77 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  73 (D) 611−1995 1525 937 −2295 −1400 −148 −2043 211 −2006 −1106 −37 −1553 1420−312 −408 1235 −1609 −2193 −1499 78 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  74 (A) 2716 −902 −2380 −2205 −2799 −1197−1975 −2459 −2081 −2736 −1895 −1520 −1895 −1844 −2201 1191 1299 −1669−3045 −2758 79 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  75 (G) −1709 −2833 2424 −409 −3781 2819 −1457 −3777 −1728−3733 −3076 −739 −2389 −1180 −2441 −1557 −1893 −3158 −3660 −3038 80 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −212 −2909 −8150 −273 −2534 −701 −1378 * *  76 (A) 2529−1119 −2614 −2330 −1245 −1983 −1829 −377 −2042 1435 −341 −1937 −2411−1873 −2088 −1266 −1059 −397 −2063 −1713 82 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  77 (W) −472 −361 −2421 −1812 −298 −1979−826 1164 −1486 −143 2485 873 −2028 −1185 −1426 −1048 −412 1116 2999−454 83 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  78(P) −1198 −1737 −2187 −2394 −3665 2006 −2550 −3630 −2743 −3756 −3008−2052 3474 −2495 −2835 −1401 −1593 −2736 −3511 −3519 84 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * *  79 (Q) −999 −1075 −2106−1568 −726 −2370 −1175 83 −1185 1373 218 −1566 −2400 2445 −1340 −1445−946 1441 −1501 −1146 85 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * *  80 (Q) −885 −779 −2609 −2018 −481 −2414 −1253 1645 −1736799 1924 −1827 −2405 2262 −1752 −1484 −821 802 −1240 −935 86 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  81 (F) −3342 −2776−4026 −4232 4354 −3545 −1431 −2315 −4038 −1801 −1900 −3299 −3780 −3350−3645 −3490 −3420 −2566 −739 349 87 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  82 (G) −998 −2100 −120 −175 −2567 2528 2174−2558 −587 −2583 −1806 1422 −1966 −461 −1038 −925 −1088 −2095 −2657−1948 88 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 83 (T) −1213 −1674 −2755 −2906 −3163 −1922 −2659 −2698 −2788 −3105−2612 −2311 −2600 −2708 −2753 −1463 3819 −2197 −3286 −3156 89 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 1378 * *  84 (I) −1286 −1279−2907 −2683 −1446 −2549 −2198 3290 −2407 −726 −534 −2386 1172 −2299−2437 −1895 −1392 283 −2302 −1913 90 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  85 (T) −493 −1105 −2189 −2267 −31011880 −2196 −2791 −2334 −3081 −2269 −1649 −2058 −2099 −2410 −719 3135−1948 −3282 −3046 91 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  86 (V) −1750 −1296 −4319 −3957 −1765 −4038 −3733 2364 −3826−619 −543 −3716 −3869 −3685 −3902 −3354 −1743 3012 −3265 −2817 92 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  87 (S) 923 −962−2348 −2422 −3132 −1207 −2248 −2850 −2440 −3140 −2285 −1624 −1954 −2158−2477 3171 −758 −1896 −3362 −3103 93 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  88 (D) −2784 −3432 4016 −1200 −4140−2466 −2197 −4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938−4046 −3710 −3552 94 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  89 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 95 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  90 (I) −1880−1493 −4193 −3724 −953 −3837 −2980 3251 −3420 257 2372 −3485 −3608 −3005−3310 −3087 −1840 617 −2373 −2155 96 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  91 (S) 2150 −939 −2407 −2415 −3075−1197 −2205 −2781 −2384 −3065 −2205 −1613 −1936 −2105 −2436 2652 −729−1850 −3306 −3049 97 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  92 (M) −979 −1455 −1242 −1122 −1434 −1860 −1131 −1171 −974−1285 4091 2176 −2226 −1017 −1187 −1166 −1086 −1063 −1929 −1345 98 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  93 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 99 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * *  94 (T) −959 −1691 −1249 −949 −2563−1747 −929 −2093 1282 −2263 −1554 −995 −2115 −600 −354 −1037 3152 −1726−2494 −2098 100 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  95 (E) −572 −1860 −208 2213 −2107 −1461 −191 −1808 199 −116−983 −127 318 1199 −269 −475 −517 −1448 −2078 −1441 101 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * *  96 (G) −2594 −2690 −3304−3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779−2839 −2981 −4004 −3668 −4222 102 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * *  97 (M) −2406 −2296 −3638 −3594 −1525 −3105−2824 −1047 −3121 −596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398−2513 −2207 103 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * *  98 (R) −2097 −2786 −2688 −1415 −3622 −2625 −555 −2964 2585−2627 −1957 −1318 −2577 −137 3015 −1979 −1791 −2732 −2469 −2363 104 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *  99 (Y) −3615−2706 −4169 −4413 2626 −4044 −396 −2535 −3993 −1939 −1985 −2747 −3930−2852 −3446 −3296 −3494 −2686 347 4252 105 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 100 (S) −897 −1462 −2333 −2543 −3185−1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360 −2483 −2703 3465 −1316−2413 −3310 −3025 106 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 101 (L) −2871 −2457 −4231 −4103 −1033 −3803 −3165 −541 −37343130 −31 −3935 −3797 −3286 −3484 −3713 −2869 −1136 −2394 −2220 107 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 102 (V) −1381−1065 −3714 −3252 −1453 −3300 −2646 1872 −3023 −615 −373 −2949 −3287−2816 −3039 −2506 1346 2750 −2489 −2087 108 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 103 (S) −897 −1462 −2333 −2543 −3185−1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360 −2483 −2703 3465 −1316−2413 −3310 −3025 109 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 104 (R) −2957 −3022 −3318 −2735 −3796 −2998 −1968 −3912 −846−3631 −3157 −2611 −3280 −1724 4056 −3026 −2913 −3650 −3096 −3185 110 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 105 (E) −1719−3572 2596 2779 −3767 −1632 −993 −3700 −1241 −3578 −2920 −234 −2167 −666−2090 −1380 −1789 −3182 −3742 −2756 111 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 106 (V) −1746 −1296 −4308 −3946 −1757−4020 −3712 2190 −3811 −614 −539 −3702 −3858 −3667 −3884 −3336 −17403098 −3250 −2803 112 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 107 (I) −2091 −1746 −3971 −3840 −1676 −3532 −3289 3684 −3581−659 −693 −3562 −3674 −3445 −3521 −3194 −2146 449 −2877 −2493 113 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 108 (A) 3438 −1472−2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447 −2798−2944 −1216 −1387 −2183 −3405 −3320 114 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 109 (D) −2784 −3432 4016 −1200 −4140−2466 −2197 −4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938−4046 −3710 −3552 115 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 110 (S) −352 2942 −2955 −2957 −2876 −1254 −2382 −2573 −2692−2927 −2128 −1827 −2001 −2405 −2607 3103 −778 −1757 −3171 −2911 116 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 111 (I) −2091−1746 −3971 −3840 −1676 −3532 −3289 3684 −3581 −659 −693 −3562 −3674−3445 −3521 −3194 −2146 449 −2877 −2493 117 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 112 (E) −2641 −3308 −896 3732 −3966−2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479 −2750−3722 −3563 −3385 118 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 113 (T) 1556 −936 −2493 −2457 −2805 −1256 −2159 −2210 −2319−2681 −1932 −1656 −1974 −2089 −2352 −598 3235 −1547 −3111 −2847 119 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 114 (C) 17842119 −2013 −1532 −1093 −1580 −1089 −436 −1322 −937 −273 1093 −1932 −1127−1472 −748 −515 1585 −1536 −1163 120 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 115 (M) 1831 2019 −2596 −2038 −605 −1979−1126 244 −1727 −359 2501 −1655 −2145 −1435 −1683 −1106 −557 1087 −1153−804 121 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *116 (Q) −987 −2211 −43 −62 −2833 2229 −691 −2616 −407 −2604 −1797 1197−1917 2260 −858 −880 −1045 −2139 −2772 −2099 122 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 117 (G) 2313 −1042 −2391 −2526−3250 2601 −2372 −2972 −2637 −3257 −2407 −1721 −2032 −2310 −2646 −662−859 −2003 −3434 −3247 123 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 118 (Q) −914 −2350 −48 1661 −2621 −1571 2504 −2400 68−2331 −1486 −201 −1796 2646 −351 −754 −865 −1984 −2463 −1787 124 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 119 (W) −517 −1294−733 −183 −1062 −1605 −234 −1037 19 −1207 −456 1435 −1690 33 756 411−454 −819 3340 1286 125 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 120 (M) 410 −469 −2417 −1828 −341 −2041 −897 195 −1513−156 3130 −1534 −2102 −1230 −1484 −1117 −507 954 −894 2253 126 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 121 (D) −2784−3432 4016 −1200 −4140 −2466 −2197 −4505 −2621 −4365 −3956 −1551 −3014−2039 −3232 −2593 −2938 −4046 −3710 −3552 127 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 122 (G) 2142 −930 −2334 −2298−3100 2237 −2139 −2842 −2302 −3074 −2187 −1557 −1909 −2010 −2397 1136−701 −1871 −3308 −3053 128 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 123 (V) −1514 −1144 −3950 −3459 1821 −3487 −2577 2274−3208 −209 −87 −3112 −3362 −2864 −3118 −2680 −1476 2426 −2194 −1786 129— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 124 (V) −1743−1294 −4292 −3873 −1511 −3988 −3433 2287 −3712 598 −319 −3626 −3774−3456 −3716 −3260 −1717 2790 −2931 −2577 130 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 125 (A) 2911 −954 −2808 −2665 −2115−1577 −2196 −575 −2445 −1646 −1202 −1906 −2208 −2218 −2451 −901 −8761294 −2727 −2394 131 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 126 (I) −1764 −1323 −4298 −3936 −1668 −3994 −3655 3337 −3783−508 −462 −3689 −3838 −3608 −3835 −3311 −1759 1847 −3164 −2747 132 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 127 (G) −1157−1705 −2169 −2375 −3654 3021 −2534 −3611 −2730 −3741 −2984 −2024 2418−2475 −2826 −1361 −1555 −2705 −3513 −3509 133 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 128 (G) −2594 −2690 −3304 −3623−4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839−2981 −4004 −3668 −4222 134 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 129 (C) −2476 5735 −4102 −4358 −3712 −2763 −3545−3518 −4167 −3859 −3569 −3631 −3363 −4030 −3832 −2793 −2860 −3158 −3464−3718 135 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *130 (D) −2784 −3432 4016 −1200 −4140 −2466 −2197 −4505 −2621 −4365 −3956−1551 −3014 −2039 −3232 −2593 −2938 −4046 −3710 −3552 136 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 131 (K) −2620 −2961−2461 −2046 −3743 −2791 −1570 −3603 3784 −3387 −2839 −2048 −3039 −1260−465 −2604 −2536 −3331 −3001 −2988 137 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 132 (N) −2171 −2655 −1458 −1748 −3334−2364 −2267 −3943 −2365 −3936 −3437 4205 −2932 −2205 −2608 −2224 −2439−3392 −3253 −2909 138 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 133 (M) −2406 −2296 −3638 −3594 −1525 −3105 −2824 −1047 −3121−596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398 −2513 −2207 139 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 134 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 140 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 135 (G) −2594 −2690 −3304 −3623−4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839−2981 −4004 −3668 −4222 141 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 136 (A) 2180 −935 −2286 −2196 −3057 1098 −2058−2796 −2174 −3021 −2134 −1516 −1898 −1906 −2302 2146 −689 −1849 −3256−2983 142 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *137 (M) −1799 −1433 −4142 −3579 −669 −3668 −2608 1558 −3293 1235 3799−3296 −3401 −2717 −3088 −2843 −1726 1156 −2002 −1868 143 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 138 (I) −2091 −1746 −3971−3840 −1676 −3532 −3289 3684 −3581 −659 −693 −3562 −3674 −3445 −3521−3194 −2146 449 −2877 −2493 144 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 139 (A) 3103 −1036 −2445 −2572 −3222 1051 −2380−2930 −2650 −3226 −2381 −1739 −2034 −2327 −2648 −664 −857 −1981 −3412−3228 145 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *140 (M) −2325 −1891 −4598 −4012 −498 −4222 −3013 1242 −3722 1864 3929−3855 −3711 −2910 −3414 −3439 −2215 −299 −2076 −2098 146 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 141 (A) 3103 −1036 −2445−2572 −3222 1051 −2380 −2930 −2650 −3226 −2381 −1739 −2034 −2327 −2648−664 −857 −1981 −3412 −3228 147 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 142 (R) −1588 −2442 −1399 −953 −3069 −2171 −708−2795 373 −2625 −1916 1858 −2357 −324 3294 −1520 −1505 −2453 −2523 −2186148 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 143 (M)−1448 −1256 −3396 −2819 −474 −3024 −1923 175 −2473 2225 2756 −2574 −2922−2063 −2375 −2153 952 −151 −1599 −1410 149 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 144 (N) −1662 −3306 2055 78 −3621 −1643−1040 −3622 −1272 −3531 −2870 3477 −2182 −724 −2071 −1371 −1757 −3092−3633 −2700 150 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 145 (I) −1066 −921 −2828 −2239 −1041 −2675 −1601 2235 −1668−455 −92 −2067 −2692 −1688 1701 −1795 −1024 1960 −1771 −1396 151 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 146 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 152 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 147 (S) 1568 −940 −2267 −2192−3082 1101 −2068 −2826 −2185 −3049 −2159 −1515 −1901 −1915 −2313 2603−694 −1866 −3279 −3006 153 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 148 (I) −1880 −1492 −4195 −3728 −963 −3841 −2991 3272−3425 246 2277 −3490 −3613 −3014 −3317 −3092 −1841 628 −2385 −2163 154 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 149 (F) −2204−1797 −3724 −3473 3206 −3383 −628 −1077 −3092 −746 3167 −2502 −3309−2372 −2792 −2535 −2120 −1245 28 2460 155 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 150 (V) 1265 −1028 −3200 −2994 −1833−2150 −2480 417 −2771 −1122 −818 −2349 −2640 −2559 −2766 −1464 −11183028 −2700 −2325 156 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 151 (Y) −3482 −2868 −3701 −3919 238 −3552 −1112 −3000 −3638−2516 −2526 −3027 −3772 −3101 −3341 −3418 −3527 −3071 −441 4711 157 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 152 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 158 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 153 (G) −2594 −2690 −3304 −3623−4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839−2981 −4004 −3668 −4222 159 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 154 (T) −359 −976 −2225 −2229 −2900 −1242 −2074−2560 −2170 −2875 −2064 −1561 −1958 −1969 −2247 1110 3375 −1760 −3152−2850 160 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *155 (I) −2091 −1746 −3971 −3840 −1676 −3532 −3289 3684 −3581 −659 −693−3562 −3674 −3445 −3521 −3194 −2146 449 −2877 −2493 161 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 156 (H) 861 −1924 −384 1010−2260 −1477 1787 −1974 1769 −1918 −1022 −120 −1566 362 697 −417 −459−1557 −2073 −1446 162 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 157 (P) −655 −1502 −711 −557 −2204 −1463 2143 −2122 −586 −2233−1445 −688 2941 −560 −941 855 −805 −1657 −2369 −1763 163 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 158 (G) −2594 −2690 −3304−3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779−2839 −2981 −4004 −3668 −4222 164 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 159 (H) −744 −2193 −114 1118 −2513 −1512 2486−2252 1178 −2183 −1308 2230 −1689 180 −233 −598 −687 −1823 −2335 −1670165 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 160 (W)−2672 −2139 −3850 −3748 941 −3611 −469 −1691 −3306 1047 −1217 −2551−3534 −2514 −2960 −2788 −2577 −1799 4205 3466 166 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 161 (K) 386 −1981 779 279−2295 −1403 −114 −2043 2059 −1991 −1082 941 −1536 1263 −211 −384 −457−1602 −2161 −1476 167 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 162 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 168 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 163 (K) −1144−2365 −912 2048 −2856 −1912 −326 −2459 2267 −2295 −1482 −556 −1989 1081334 −1013 −1014 −2093 −2324 −1881 169 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 164 (D) −1091 −2610 2941 174 −2957 −1527−595 −2750 1084 −2696 −1877 −176 −1885 −206 −1006 740 −1098 −2288 −2880−2105 170 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *165 (L) −2387 −1922 −4674 −4155 −617 −4366 −3250 1889 −3865 2650 558−4023 −3847 −3098 −3586 −3647 −2296 −38 −2247 −2224 171 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 166 (N) −1021 −2427 1806 133−2870 −1499 −635 −2647 −521 −2640 −1825 2171 −1874 −255 −1124 −860 2122−2184 −2853 −2090 172 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 167 (I) −1830 −1390 −4327 −3873 −1210 −3994 −3274 2967 −36781259 −30 −3633 −3730 −3283 −3604 −3249 −1791 1570 −2661 −2417 173 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 168 (V) −1771−1603 −3750 −3689 −2037 −3050 −3231 403 −3479 −1154 −1076 −3246 −3399−3383 −3437 −2628 −1917 3536 −3074 −2677 174 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 169 (S) −897 −1462 −2333 −2543 −3185−1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360 −2483 −2703 3465 −1316−2413 −3310 −3025 175 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 170 (A) 2440 −824 −2371 −2082 −1993 −1344 −1704 −1264 −1899−1832 −1137 −1517 −1946 −1674 −2005 1075 −641 1474 −2390 −2055 176 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 171 (F) −3342−2776 −4026 −4232 4354 −3545 −1431 −2315 −4038 −1801 −1900 −3299 −3780−3350 −3645 −3490 −3420 −2566 −739 349 177 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 172 (E) −2641 −3308 −896 3732 −3966−2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479 −2750−3722 −3563 −3385 178 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 173 (A) 2966 −1031 −2429 −2551 −3222 1544 −2368 −2934 −2633−3225 −2377 −1727 −2028 −2309 −2637 −656 −850 −1980 −3412 −3224 179 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 174 (V) −1769−1342 −4255 −3793 −1216 −3901 −3162 1633 −3589 1486 −51 −3537 −3667−3214 −3518 −3143 −1731 2692 −2609 −2345 180 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 175 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 181 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 176 (Q) −729 −2116 −413 1096 −2484 −1587 1599 −2186 1695 −2094−1219 −223 −1698 2418 90 −599 −649 −1770 −2213 −1615 182 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 177 (W) −1652 −1707 −2340−1879 1996 −2733 2013 −1398 1758 −1386 −938 −1641 −2751 −1364 −1762−1780 −1577 −1325 3577 2136 183 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 178 (T) −421 −753 −1251 −704 −846 −1670 −535 894−548 −690 −1 1376 −1791 −421 −846 373 1461 858 −1236 −812 184 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 179 (H) 1498 −1593−504 15 −1895 −1484 2279 −1559 1119 −1640 −810 −242 −1611 194 −171 −462815 −1231 −1914 −1340 185 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 180 (G) −1515 −2130 −1298 −1450 −2658 3285 2212 −3276−1691 −3291 −2638 −1524 −2562 −1662 −1925 −1600 −1764 −2713 −2804 −2234186 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 181 (K)−528 −2010 1346 1082 −2329 −1408 −118 −2080 1475 −2018 −1108 1161 −1543331 1052 −394 −471 −1632 −2181 −1494 187 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 182 (M) −1894 −1521 −4170 −3679 −840−3793 −2866 2827 −3360 375 3445 −3437 −3555 −2902 −3223 −3028 −1846 470−2249 −2059 188 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 183 (T) −670 −1758 1731 −141 −2591 −1399 −691 −2319 −499 −2384−1543 −387 −1786 −316 −1016 1576 2044 −1811 −2624 −1981 189 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 184 (E) 345 −2074 9251994 −2378 −1408 −177 −2135 922 −2084 −1183 −38 641 264 −356 −444 −536−1690 −2261 −1556 190 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 185 (E) −1493 −2900 93 3174 −2903 −1743 1987 −3042 −646 −2957−2238 −411 −2146 −506 −1121 −1272 −1503 −2629 −2905 −2134 191 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 186 (D) −1293−2959 2673 2121 −3219 −1546 −713 −3043 −707 −2974 −2191 −158 −1967 −342−1394 −1043 701 −2567 −3172 −2311 192 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 187 (F) −1137 −905 −3250 −2707 2365−2647 −1016 −34 −2336 1239 267 −2150 −2626 −1861 −2133 −1752 −1069 1461−599 1844 193 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 39445 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *188 (K) −479 −1713 −409 1031 −1925 −1467 1755 −1650 1844 −349 −827 −140−1556 319 −75 −403 −411 −1301 −1900 843 194 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 189 (G) 433 −2144 52 1047 −2717 2303−615 −2467 −442 −2482 −1655 1123 −1828 −233 −995 −763 −923 −2000 −2710−2005 195 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *190 (V) −1752 −1320 −4254 −3806 −1311 −3916 −3232 1701 −3614 1188 −140−3551 −3693 −3280 −3568 −3166 −1718 2833 −2703 −2409 196 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 191 (E) −1199 −1750 −7342668 −1820 −2038 −1068 1892 −867 −1273 −897 −922 −2295 −797 −1238 −1340−1197 −426 −2325 −1789 197 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 192 (C) −1182 3528 −1398 −620 −2541 −2038 −358 −2093 1181−2037 −1272 −747 −2070 1553 2213 −1123 −1038 −1817 −2142 −1774 198 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 193 (N) −1478−2527 −261 −403 −2011 −1837 2032 −2925 −735 −2845 −2195 3635 −2259 −721−1085 −1352 −1546 −2522 −2307 −1431 199 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 194 (A) 3438 −1472 −2846 −3040 −3287−1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447 −2798 −2944 −1216 −1387−2183 −3405 −3320 200 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 195 (C) −1220 4911 −3609 −3314 −1440 −2525 −2482 1565 −2922−706 −544 −2678 −2896 −2710 −2836 −1869 −1375 379 −2371 −1957 201 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 196 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 202 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 197 (G) −477 −1115 −1983 −2189−3315 3154 −2272 −3172 −2506 −3387 −2522 −1599 −2042 −2177 −2583 1217−905 −2130 −3477 −3225 203 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 198 (A) 1653 −1347 −705 −249 −1969 −1385 −477 −1629 −159−1759 −935 −434 1285 1404 −586 −450 1019 −1243 −2070 −1522 204 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 199 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 205 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 200 (S) 1870 −938 −2270 −2183−3068 1488 −2056 −2810 −2168 −3032 −2144 −1511 −1898 −1901 −2300 2236−690 −1857 −3265 −2990 206 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 201 (C) −2476 5735 −4102 −4358 −3712 −2763 −3545 −3518−4167 −3859 −3569 −3631 −3363 −4030 −3832 −2793 −2860 −3158 −3464 −3718207 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 202 (G)−2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320−3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 208 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 203 (G) −2594 −2690 −3304−3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779−2839 −2981 −4004 −3668 −4222 209 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 204 (M) −2406 −2296 −3638 −3594 −1525 −3105−2824 −1047 −3121 −596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398−2513 −2207 210 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 205 (Y) −3590 −2700 −4146 −4379 2092 −4028 −404 −2517 −3963−1928 −1973 −2744 −3921 −2845 −3431 −3284 −3474 −2669 336 4423 211 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 206 (T) −1213−1674 −2755 −2906 −3163 −1922 −2659 −2698 −2788 −3105 −2612 −2311 −2600−2708 −2753 −1463 3819 −2197 −3286 −3156 212 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 207 (A) 3438 −1472 −2846 −3040 −3287−1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447 −2798 −2944 −1216 −1387−2183 −3405 −3320 213 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 208 (N) −2171 −2655 −1458 −1748 −3334 −2364 −2267 −3943 −2365−3936 −3437 4205 −2932 −2205 −2608 −2224 −2439 −3392 −3253 −2909 214 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 209 (T) −1213−1674 −2755 −2906 −3163 −1922 −2659 −2698 −2788 −3105 −2612 −2311 −2600−2708 −2753 −1463 3819 −2197 −3286 −3156 215 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 210 (M) −2355 −1988 −4343 −3834 −504−4051 −2868 105 −3385 1451 4460 −3680 −3671 −2806 −3171 −3327 −2274 −474−2039 −1925 216 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 211 (S) 2150 −939 −2407 −2415 −3075 −1197 −2205 −2781 −2384−3065 −2205 −1613 −1936 −2105 −2436 2652 −729 −1850 −3306 −3049 217 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 212 (S) −344−979 −2190 −2162 −2959 −1227 −2042 −2651 −2116 −2934 −2100 −1526 −1941−1909 −2222 2940 1775 −1804 −3187 −2882 218 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 213 (A) 3048 −932 −2480 −2533 −3075−1200 −2274 −2765 −2501 −3071 −2221 −1658 −1948 −2205 −2512 1225 −739−1842 −3322 −3078 219 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 214 (I) −1924 −1546 −4067 −3658 2312 −3663 −2081 3030 −3367150 99 −3197 −3492 −2821 −3179 −2894 −1877 293 −1445 −692 220 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 215 (E) −2641−3308 −896 3732 −3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959−1842 −2560 −2479 −2750 −3722 −3563 −3385 221 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 216 (A) 2389 −814 −2506 −2162−1696 −1545 −1698 −499 −1942 −1398 −813 −1640 −2076 −1723 −2027 −8061148 1559 −2200 −1856 222 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 217 (M) −2576 −2118 −4725 −4165 −461 −4430 −3165 99 −38112513 3454 −4075 −3839 −2978 −3488 −3704 −2457 −591 −2111 −2145 223 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 218 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 224 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 219 (M) −2313 −1968 −4258 −3765−518 −3966 −2806 98 −3289 1292 4523 −3599 −3636 −2769 −3097 −3249 −2243−457 −2026 −1874 225 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 220 (S) −897 −1462 −2333 −2543 −3185 −1640 −2474 −3294 −2686−3497 −2780 −1973 −2360 −2483 −2703 3465 −1316 −2413 −3310 −3025 226 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 221 (L) −2631−2159 −4786 −4228 −462 −4506 −3231 96 −3878 2828 2482 −4157 −3880 −3016−3541 −3793 −2509 −608 −2134 −2182 227 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 222 (P) −1501 −1778 −2473 −2371 −1710−2311 −2045 −1321 −2060 827 −1068 −2173 3594 −2082 −2130 −1799 −1699−1373 −2373 −1942 228 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 223 (Y) −1068 −1670 −865 −836 −631 1198 −767 −1828 −1059 −1914−1304 692 −2203 −906 −1387 −1136 −1163 −1566 −1185 3670 229 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 224 (S) −897 −1462−2333 −2543 −3185 −1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360 −2483−2703 3465 −1316 −2413 −3310 −3025 230 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 225 (S) 1172 −954 −2367 −2422 −3120−1204 −2237 −2835 −2426 −3122 −2265 −1621 −1948 −2145 −2467 3107 −749−1884 −3349 −3092 231 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 226 (S) −342 −975 −2176 −2124 −2912 −1229 −2003 −2594 −2067−2878 −2048 −1510 −1936 −1866 −2184 2553 2492 −1773 −3143 −2833 232 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 227 (M) −720−1440 −710 −343 −1228 −1693 2436 −1209 −132 −1364 3099 1904 −1852 −183−458 −776 −680 −1004 −1540 −890 233 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 228 (P) 2240 −1100 −2241 −2293 −3037 −1346−2188 −2683 −2317 −2986 −2210 −1663 3041 −2093 −2391 −722 −895 −1893−3243 −2998 234 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 229 (A) 2958 −1235 −1299 −1377 −2868 −1345 −1673 −2580 −1661−2843 −2054 1555 −1995 −1468 −1921 −715 −888 −1871 −3064 −2630 235 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 230 (E) −509−1046 −884 1564 −1116 −1669 −441 −485 −283 250 −206 −577 689 −200 −656−670 −459 1290 −1467 −995 236 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 231 (D) −1203 −2412 2595 −117 −3286 −1536 −1057−3176 −1165 −3186 −2436 −428 −2068 −736 −1824 2377 −1366 −2578 −3334−2552 237 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *232 (Q) 954 −1983 −100 971 −2337 177 −267 −2067 81 −2060 −1189 −125−1637 2600 −418 −514 −597 −1649 −2268 −1597 238 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 233 (E) −2641 −3308 −896 3732−3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479−2750 −3722 −3563 −3385 239 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 234 (K) −2620 −2961 −2461 −2046 −3743 −2791 −1570−3603 3784 −3387 −2839 −2048 −3039 −1260 −465 −2604 −2536 −3331 −3001−2988 240 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *235 (R) 377 −1802 −415 988 −2095 −1474 −95 −1786 1452 −1785 −911 −135−1560 343 1555 −409 −431 376 −1986 −1375 241 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 236 (D) 1083 −1565 2662 −244 −1941 −1573−679 612 −527 −1651 −980 −490 −1869 −358 −1003 −771 −766 −903 −2208−1633 242 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *237 (E) −1225 −2868 1894 1948 −3149 −1532 −671 −2975 −630 −2902 −2101−150 −1935 −293 −1299 1884 −1241 −2496 −3093 −2248 243 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 238 (C) 1375 3262 −2620−2108 −827 −1866 −1267 1631 −1811 −599 −10 −1674 −2137 −1531 −1786 −1034790 249 −1361 −1010 244 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 239 (E) 635 −1796 1055 1761 −2018 −1464 −263 1191 28−1767 −946 −148 −1637 135 −481 −520 −553 −1300 −2077 −1441 245 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 240 (E) 593 −2044−252 2548 −2437 −1542 −329 −2133 151 −2120 −1274 −244 −1738 89 946 −646−717 −1734 −2305 −1686 246 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 241 (S) 1884 −835 −1962 −1576 −1634 −1436 −1320 1041−1409 −1453 −781 −1293 −1922 −1241 −1606 1973 −597 −669 −2036 −1656 247— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 242 (G) 2267−1043 −2388 −2526 −3253 2642 −2373 −2975 −2639 −3260 −2410 −1722 −2033−2311 −2648 −663 −860 −2005 −3436 −3250 248 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 243 (R) −876 −2087 −829 1490 −2474 −1766−229 −2106 1269 −44 −1198 −424 −1829 205 2225 −775 −768 −1753 −2143−1647 249 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *244 (V) 2339 −967 −2970 −2766 −1878 −1847 −2252 32 −2541 −1299 −918−2087 −2399 −2316 −2545 −1157 −971 2345 −2605 −2251 250 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 245 (I) −1827 −1398 −4307−3831 −1099 −3939 −3142 2286 −3619 1835 69 −3579 −3671 −3177 −3511 −3178−1781 1918 −2524 −2310 251 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 246 (V) −1178 −1448 −1943 −1452 −1776 −2261 −1140 −2271866 −1260 −816 −1444 −2448 −902 −540 −1496 −1176 2697 −2161 −1764 252 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 247 (E) −508−1976 840 1547 −2280 −1393 −117 −2029 1400 −1984 −1077 1158 −1531 330−253 −378 −454 262 −2163 −1471 253 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 248 (M) 1703 −991 −2901 −2342 −528 −2567 −1550166 −2031 1544 2668 −2104 −2591 −1715 −2010 −1685 −1052 −12 −1442 −1177254 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 249 (I)−1947 −1516 −4385 −3885 −916 −4013 −3118 2193 −3656 2186 257 −3656 −3687−3109 −3494 −3250 −1889 1383 −2397 −2258 255 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 250 (E) −1322 −2647 −272 2491 −3071−1811 −576 −2759 2306 −2633 −1854 −464 −2066 −175 −177 −1144 −1256 −2368−2692 −2140 256 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 251 (K) −1395 −2059 −1711 −1014 −2215 −2218 −641 −1709 3021−1652 2578 −1075 −2303 −282 287 −1423 −1283 −1603 −2159 −1803 257 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 252 (D) −1285−2888 2677 176 −3210 1189 −737 −3047 −715 −2977 −2195 −190 −1979 2106−1379 −1050 −1315 −2564 −3161 −2320 258 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 253 (I) −2073 −1632 −4434 −3975 −911−4130 −3238 3164 −3706 1451 244 −3779 −3785 −3187 −3557 −3413 −2021 546−2449 −2273 259 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 254 (K) −1570 −2144 −1887 −1191 −2098 −2363 −750 −1603 3034938 −1112 −1231 −2436 −408 215 −1616 −1443 −1580 −2166 −1804 260 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 255 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 261 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 256 (R) −928 −1705 −1507 −1055−2761 −1730 −896 −2490 −44 −2489 −1723 −1042 −2102 −543 2614 2258 −1053−1998 −2546 −2158 262 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 257 (D) −1280 −2865 3154 175 −3194 −1547 −743 −3034 −728 −2971−2194 −190 −1979 1342 −1391 553 −1316 −2552 −3161 −2317 263 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 258 (I) −1997 −1562−4355 −3927 −1042 −4066 −3261 3343 −3654 937 97 −3718 −3783 −3239 −3555−3364 −1959 702 −2549 −2295 264 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 259 (M) −2252 −1821 −4572 −3991 −530 −4164 −29902068 −3709 1993 3197 −3808 −3685 −2916 −3406 −3378 −2149 −172 −2084−2091 265 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *260 (T) −1213 −1674 −2755 −2906 −3163 −1922 −2659 −2698 −2788 −3105−2612 −2311 −2600 −2708 −2753 −1463 3819 −2197 −3286 −3156 266 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 261 (R) −2131−2786 −2704 −1460 −3618 −2638 −587 −2976 1735 −2645 −1985 −1353 −2603−173 3492 −2020 −1828 −2748 −2484 −2384 267 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 262 (K) −1349 −2635 −381 2083 −3083−1857 −565 −2750 2690 −2612 −1837 −514 −2090 −161 −61 −1178 −1271 −2369−2655 −2138 268 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 263 (A) 2821 −932 −2451 −2472 −3065 −1198 −2233 −2763 −2434−3056 −2201 −1633 −1940 −2147 −2468 1831 −730 −1840 −3305 −3055 269 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 264 (F) −2063−1686 −4037 −3677 3437 −3644 −1706 2063 −3359 135 67 −3095 −3486 −2739−3127 −2876 −2012 −83 −1038 −158 270 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 265 (E) −2641 −3308 −896 3732 −3966−2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479 −2750−3722 −3563 −3385 271 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 266 (N) −1662 −3306 2055 78 −3621 −1643 −1040 −3622 −1272−3531 −2870 3477 −2182 −724 −2071 −1371 −1757 −3092 −3633 −2700 272 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 267 (A) 3438−1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447−2798 −2944 −1216 −1387 −2183 −3405 −3320 273 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 268 (I) −1760 −1307 −4325 −3962−1735 −4042 −3726 3135 −3828 −579 −515 −3722 −3869 −3673 −3896 −3359−1752 2276 −3240 −2806 274 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 269 (T) 1428 −904 −2334 −2158 −2747 −1206 −1940 −2392−2037 −2678 −1846 −1504 −1896 −1809 −2163 902 3001 −1635 −2999 −2705 275— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 270 (V) −1745−1300 −4286 −3858 −1446 −3967 −3370 2358 −3688 852 −261 −3606 −3749−3403 −3673 −3232 −1717 2643 −2856 −2524 276 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 271 (V) −1404 −1072 −3766 −3305 −1464−3356 −2696 2276 −3080 −616 −379 −3001 −3325 −2870 −3091 −2563 1344 2521−2516 −2113 277 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 272 (M) 866 −1113 −2656 −2412 −1322 −1920 −1883 −487 −2061−587 4451 −1950 −2387 −1928 −2078 −1220 −1053 −498 −2134 −1803 278 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 273 (A) 2601−957 −2898 −2711 −1943 −1740 −2211 −165 −2487 −1406 −1001 −2008 −2320−2260 −2494 −1053 −929 1990 −2626 −2279 279 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 274 (L) −1171 −983 −3266 −2733 −796−2795 −1888 590 −2418 2001 198 −2418 −2816 −2106 −2362 −1944 965 1777−1724 −1426 280 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 275 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 281 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 276 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 282 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 277 (S) −897 −1462 −2333 −2543−3185 −1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360 −2483 −2703 3465−1316 −2413 −3310 −3025 283 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 278 (T) −1213 −1674 −2755 −2906 −3163 −1922 −2659−2698 −2788 −3105 −2612 −2311 −2600 −2708 −2753 −1463 3819 −2197 −3286−3156 284 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *279 (N) −2171 −2655 −1458 −1748 −3334 −2364 −2267 −3943 −2365 −3936−3437 4205 −2932 −2205 −2608 −2224 −2439 −3392 −3253 −2909 285 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 280 (A) 3134 −934−2491 −2567 −3083 −1203 −2300 −2766 −2540 −3082 −2237 −1672 −1954 −2240−2537 874 −747 −1844 −3333 −3093 286 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 281 (V) −984 −1045 −3169 −2909 −1709−2304 −2404 531 −2643 −988 −697 −2378 −2722 −2480 −2661 −1601 1504 3014−2588 −2201 287 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 282 (L) −2631 −2159 −4786 −4228 −462 −4506 −3231 96 −3878 28282482 −4157 −3880 −3016 −3541 −3793 −2509 −608 −2134 −2182 288 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 283 (H) −3205−3079 −2723 −2890 −2110 −3046 5295 −4135 −2617 −3813 −3561 −2886 −3482−2833 −2620 −3291 −3356 −3895 −2397 −1681 289 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 284 (L) −1623 −1338 −3726 −3164−251 −3255 −1820 1373 −2808 2371 514 −2785 −3086 −2281 −2613 −2389 −1543−161 −1311 1782 290 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 285 (L) −2333 −1873 −4640 −4127 −650 −4326 −3241 2176 −38432519 523 −3982 −3833 −3105 −3579 −3604 −2247 56 −2268 −2230 291 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 286 (A) 3438 −1472−2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447 −2798−2944 −1216 −1387 −2183 −3405 −3320 292 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 287 (M) −1886 −1507 −4178 −3693 −877−3806 −2901 3008 −3380 335 3109 −3451 −3570 −2934 −3251 −3044 −1840 524−2288 −2089 293 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 288 (A) 3438 −1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028−3257 −2662 −2236 −2447 −2798 −2944 −1216 −1387 −2183 −3405 −3320 294 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 289 (H) −1490−2484 −362 −476 −1816 −1880 4320 −2854 −684 −2770 −2133 2185 −2285 −728−1000 −1377 −1550 −2475 −2146 −1255 295 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 290 (A) 2439 −911 −2326 −2131 −2811−1197 −1934 −2480 −2011 −2745 −1898 −1490 −1888 −1785 −2153 1898 1073−1682 −3044 −2749 296 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 291 (I) 2038 −985 −3388 −2919 −1320 −2893 −2277 2155 −2677−587 −297 −2593 −2992 −2450 −2697 −2087 −1208 1681 −2229 −1846 297 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 292 (G) −1243−2769 311 1902 −3172 1980 −744 −2992 −697 −2936 −2152 1923 −1974 −377−1331 −1030 −1284 2506 −3125 −2308 298 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 293 (V) −1738 −1298 −4281 −3921 −1737−3979 −3665 1917 −3774 −601 −528 −3671 −3834 −3628 −3843 −3293 −17353205 −3215 −2770 299 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 294 (E) −833 −2344 1092 2412 −2643 −1464 −386 −2413 −146 −2369−1505 −96 562 29 −717 −666 862 −1966 −2562 −1818 300 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 295 (W) −1380 −1116 −3614−3026 1322 −2981 −1582 1966 −2661 1775 556 −2562 −2865 −2117 −2424 −2098−1302 −187 2908 −629 301 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 296 (T) −350 −973 −2204 −2178 −2893 −1236 −2035 −2561−2117 −2862 −2043 −1536 −1946 −1916 −2214 1618 3198 −1758 −3137 −2831302 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 297 (L)−1443 −1269 −3144 −2576 −528 −3014 −1816 1945 −2155 2102 508 −2422 −28991193 −2133 −2129 −1369 −50 −1616 −1384 303 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 298 (D) −1826 −3682 3559 1199 −3883 1662−1073 −3846 −1391 −3720 −3110 −272 −2222 −760 −2283 −1471 −1913 −3321−3864 −2864 304 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 299 (D) −2784 −3432 4016 −1200 −4140 −2466 −2197 −4505 −2621−4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938 −4046 −3710 −3552 305 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 300 (F) −3342−2776 −4026 −4232 4354 −3545 −1431 −2315 −4038 −1801 −1900 −3299 −3780−3350 −3645 −3490 −3420 −2566 −739 349 306 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 301 (Q) −1048 −2608 205 2170 −2893 −1535−505 −2680 −255 −2604 −1769 1814 −1849 2272 −789 −848 −1028 −2228 −2770−2013 307 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *302 (R) 1083 −1687 691 135 −2058 −1406 −178 −1755 214 −1793 −924 −145−1553 247 1670 −383 1217 −1367 −2031 −1404 308 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 303 (I) −1915 −1536 −4077 −36672027 −3678 −2155 3137 −3381 144 94 −3225 −3506 −2848 −3202 −2914 −1871345 −1522 −791 309 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 304 (R) −689 −2015 −494 24 −2395 −1582 −184 −2087 444 −2020−1151 1161 −1687 1832 2131 626 −614 −1684 −2156 −1573 310 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 305 (D) 387 −1967 16001359 −2275 −1391 1561 −2025 282 −1976 −1067 −25 −1525 342 1024 −369 −443−1584 −2152 −1462 311 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 306 (R) −1460 −2315 −1793 −887 −2832 −2237 −431 −2288 2193−2199 −1473 −946 −2245 −20 2706 −1394 −1275 591 −2248 −1961 312 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 307 (V) −941 −1027−3099 −2832 −1692 −2234 −2324 470 −2565 −1003 −695 −2305 −2663 −2399−2587 −1527 1858 2876 −2536 −2152 313 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 308 (P) −2931 −2878 −3420 −3706 −4181−2925 −3468 −4621 −3859 −4490 −4165 −3491 4225 −3781 −3695 −3182 −3279−4087 −3594 −4064 314 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 309 (V) −1090 −1215 −2097 −1824 −819 −2221 2699 −287 −1392−1027 −591 −1674 −2482 −1446 −1482 −1482 −1143 2879 −1420 −707 315 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 310 (L) −2439−1972 −4702 −4181 −588 −4401 −3258 1582 −3881 2757 587 −4061 −3862 −3093−3590 −3689 −2344 −130 −2230 −2217 316 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 311 (C) 2157 4166 −3012 −2973 −2780 1022−2337 −2398 −2724 −2744 −1930 −1786 −1943 −2372 −2623 −540 −692 −1624−3091 −2881 317 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 312 (D) −1732 −3453 3468 99 −3733 −1645 −1066 −3747 −1356−3641 −3008 1690 −2201 −755 −2209 −1416 −1833 −3208 −3752 −2776 318 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 313 (L) −2477−2023 −4713 −4122 1592 −4329 −2920 72 −3835 2593 2472 −3948 −3754 −2914−3466 −3550 −2350 −634 −1927 −1830 319 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 314 (K) −2620 −2961 −2461 −2046 −3743−2791 −1570 −3603 3784 −3387 −2839 −2048 −3039 −1260 −465 −2604 −2536−3331 −3001 −2988 320 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 315 (P) −2931 −2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859−4490 −4165 −3491 4225 −3781 −3695 −3182 −3279 −4087 −3594 −4064 321 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 316 (S) −897−1462 −2333 −2543 −3185 −1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360−2483 −2703 3465 −1316 −2413 −3310 −3025 322 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 317 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 323 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 318 (K) 2 −2257 −1073 −374 −2740 −1908 −278 −2339 2328 −2192−1373 −562 −1953 2273 1344 −952 −933 −1980 −2234 −1799 324 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 319 (Y) −3482 −2868−3701 −3919 238 −3552 −1112 −3000 −3638 −2516 −2526 −3027 −3772 −3101−3341 −3418 −3527 −3071 −441 4711 325 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 320 (M) −1559 −1267 −3829 −3380 −1103−3357 −2655 805 −3067 −64 3046 −3065 −3326 −2779 −3011 −2591 −1556 2855−2312 −1998 326 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 321 (M) 1225 −469 −2256 −1679 1656 −1926 −870 90 −1396 −2102763 −1424 −2028 −1129 −1411 −1008 712 154 −951 −586 327 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 322 (T) −738 −2094 −84 1704−2416 −1495 −317 −2135 61 −2127 −1275 −163 −1704 1857 −405 −613 1930−1734 −2331 −1668 328 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 323 (D) −1746 −3458 3540 90 −3744 −1650 −1081 −3767 −1381−3662 −3036 1386 −2211 −772 −2239 −1429 −1850 −3226 −3765 −2789 329 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 324 (L) −2451−1983 −4707 −4186 −582 −4409 −3259 1510 −3884 2778 592 −4069 −3865 −3091−3590 −3698 −2355 −150 −2226 −2214 330 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 325 (H) −2923 −2573 −2959 −2926 826−3449 4553 −2508 −2463 −2054 −1948 −2279 −3499 −2191 −2397 −2761 −2855−2540 123 2920 331 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 326 (K) 373 −1957 −342 1025 −2297 −1472 −98 −2018 2111 −1954−1056 906 −1570 352 685 −424 −473 −1592 −2105 −1469 332 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 327 (V) 1739 −1008 −3509−3043 −1376 −3028 −2406 1765 −2807 −615 −334 −2718 −3093 −2585 −2823−2226 −1263 2376 −2322 −1931 333 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 328 (G) −2594 −2690 −3304 −3623 −4328 3747−3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004−3668 −4222 334 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 329 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 335 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 330 (I) −1758−1302 −4331 −3970 −1756 −4054 −3748 2976 −3840 −603 −533 −3731 −3877−3693 −3914 −3372 −1750 2505 −3265 −2824 336 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 331 (P) −2931 −2878 −3420 −3706 −4181−2925 −3468 −4621 −3859 −4490 −4165 −3491 4225 −3781 −3695 −3182 −3279−4087 −3594 −4064 337 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 332 (Q) 1795 −1440 −730 −492 −2453 682 −812 −2151 −508 −2256−1426 −624 −1796 2666 −901 −590 −689 −1636 −2510 −1971 338 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 333 (V) −1771 −1603−3750 −3689 −2037 −3050 −3231 403 −3479 −1154 −1076 −3246 −3399 −3383−3437 −2628 −1917 3536 −3074 −2677 339 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 334 (M) −2355 −1988 −4343 −3834 −504−4051 −2868 105 −3385 1451 4460 −3680 −3671 −2806 −3171 −3327 −2274 −474−2039 −1925 340 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 335 (K) −2620 −2961 −2461 −2046 −3743 −2791 −1570 −3603 3784−3387 −2839 −2048 −3039 −1260 −465 −2604 −2536 −3331 −3001 −2988 341 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 336 (Y) −1187−974 −3186 −2638 −117 −2732 −1255 1905 −2270 73 1977 −2217 −2699 −1882−2144 −1841 −1124 71 −907 3254 342 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 337 (L) −2871 −2457 −4231 −4103 −1033 −3803−3165 −541 −3734 3130 −31 −3935 −3797 −3286 −3484 −3713 −2869 −1136−2394 −2220 343 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 338 (L) −2871 −2457 −4231 −4103 −1033 −3803 −3165 −541 −37343130 −31 −3935 −3797 −3286 −3484 −3713 −2869 −1136 −2394 −2220 344 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 339 (K) −864−1785 −860 −366 −2128 −1763 −407 −1612 2624 −1800 −1045 629 −1900 −28 62−851 −805 1127 −2064 −1581 345 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 340 (N) 602 −1686 −275 1008 −1926 −1415 1528 −1618244 −1673 −815 1897 −1530 299 −244 −371 −391 322 −1934 −1306 346 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 341 (G) −1709−2639 1362 −690 −3785 3257 −1671 −3805 −1946 −3792 −3137 −980 −2480−1424 −2576 −1630 −1936 −3150 −3628 −3155 347 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 342 (F) −942 −799 −2828 −2226 1797−2476 −1269 1109 581 1793 516 −1952 −2453 −1557 −1815 −1558 −875 52−1138 −794 348 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 343 (L) −2451 −1983 −4707 −4186 −582 −4409 −3259 1510 −38842778 592 −4069 −3865 −3091 −3590 −3698 −2355 −150 −2226 −2214 349 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 344 (H) −3205−3079 −2723 −2890 −2110 −3046 5295 −4135 −2617 −3813 −3561 −2886 −3482−2833 −2620 −3291 −3356 −3895 −2397 −1681 350 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 345 (G) −2594 −2690 −3304 −3623−4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839−2981 −4004 −3668 −4222 351 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 346 (D) −2784 −3432 4016 −1200 −4140 −2466 −2197−4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938 −4046 −3710−3552 352 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *347 (C) 774 4452 −2162 −1688 −1962 −1478 −1302 −1474 −944 −1796 −1088−1351 −1979 −1147 1684 −732 −719 −1116 −2225 −1881 353 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 348 (L) −2387 −1922 −4674−4155 −617 −4366 −3250 1889 −3865 2650 558 −4023 −3847 −3098 −3586 −3647−2296 −38 −2247 −2224 354 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 349 (T) −1213 −1674 −2755 −2906 −3163 −1922 −2659 −2698−2788 −3105 −2612 −2311 −2600 −2708 −2753 −1463 3819 −2197 −3286 −3156355 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 350 (C)−1489 2972 −4007 −3563 −1524 −3541 −2939 2612 −3350 −617 −413 −3224−3470 −3129 −3335 −2770 −1475 2269 −2657 −2248 356 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 351 (T) −364 −979 −2232 −2250−2904 −1245 −2090 −2559 −2191 −2881 −2075 −1571 −1964 −1991 −2260 9053428 −1762 −3159 −2858 357 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 352 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761−3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222358 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 353 (K)−1716 −2632 −2004 −1008 −3336 −2379 −444 −2764 2775 −2484 −1756 −1035−2357 2151 1811 −1592 −1477 −2481 −2391 −2172 359 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 354 (T) −1213 −1674 −2755−2906 −3163 −1922 −2659 −2698 −2788 −3105 −2612 −2311 −2600 −2708 −2753−1463 3819 −2197 −3286 −3156 360 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 355 (V) −1771 −1339 −4275 −3816 −1235 −3919−3194 2139 −3617 1520 −66 −3558 −3681 −3244 −3547 −3164 −1733 2390 −2634−2369 361 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *356 (A) 3438 −1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662−2236 −2447 −2798 −2944 −1216 −1387 −2183 −3405 −3320 362 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 357 (E) −2641 −3308−896 3732 −3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842−2560 −2479 −2750 −3722 −3563 −3385 363 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 358 (N) −823 −1917 −96 1188 −2187 −1547−506 −1711 −265 −1955 −1191 2711 −1815 −144 −747 −757 −815 1140 −2297−1666 364 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *359 (L) −2153 −1779 −4360 −3884 −675 −3965 −3012 392 −3561 2726 467−3673 −3662 −2955 −3355 −3239 −2102 1281 −2207 −2099 365 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 360 (E) 1136 −2084 −175 2027−2436 −1510 −274 −2147 −1525 −2118 −1254 −175 −1692 152 −251 −593 −670−1736 −2296 −1650 366 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 361 (H) 893 −1761 1357 214 −2092 −1387 1862 −1810 229 −1825−942 −83 −1527 293 −273 640 793 −1409 −2050 −1397 367 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 362 (I) 608 −458 −2776 −21761666 −2202 −1113 1712 −1836 −222 338 −1782 −2245 −1512 −1731 −1292 8671366 −1036 −684 368 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 363 (P) −922 −1912 1681 −141 −2123 −1604 −687 −1787 −550 187−1245 −427 2677 −363 −1049 −882 −947 −1524 −2338 −1711 369 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 364 (D) −1692 −36053364 1256 −3770 −1599 −957 −3700 −1216 −3569 −2909 1025 −2138 −628 −2083−1346 −1761 −3174 −3765 −2738 370 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 365 (Q) −877 −1646 −633 499 −1610 −1781 −505−1210 −63 1648 −649 −558 −1931 2241 −360 −907 −814 −1097 −1882 −1385 371— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 366 (P) −648−2019 1139 203 −2354 −1436 −285 −2089 29 −2086 −1217 −114 1965 1445 −492−529 1244 −1672 −2300 −1616 372 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 367 (R) −422 −1009 −851 −304 1406 −1496 −183 −740147 −894 −230 −440 775 21 2009 −539 −381 −568 −1136 −521 373 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −23 −6560 −7602 −894 −1115 −341 2249 * * 368 (D) 1472 −1668 1835−70 −2356 −1385 −511 −2062 −246 −2128 −1275 −318 1353 −118 −746 −526 425−1602 −2380 −1752 374 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 369 (G) −1044 −2230 2141 −100 −3222 2291 −982 −3045 −1033−3050 −2258 −395 −1985 −644 −1669 858 −1207 −2428 −3250 −2493 375 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 370 (Q) −2562−2904 −1886 −1971 −3251 −2661 −2079 −3690 −1565 −3469 −3081 −2107 −30914371 −1665 −2585 −2674 −3411 −3077 −2821 376 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 371 (D) −1275 −2955 2862 1330 −3205−1556 −670 −3029 1509 −2936 −2141 −158 −1955 −290 −1213 −1025 −1281−2554 −3111 −2272 377 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 372 (V) −1738 −1298 −4281 −3921 −1737 −3979 −3665 1917 −3774−601 −528 −3671 −3834 −3628 −3843 −3293 −1735 3205 −3215 −2770 378 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 373 (I) −2091−1746 −3971 −3840 −1676 −3532 −3289 3684 −3581 −659 −693 −3562 −3674−3445 −3521 −3194 −2146 449 −2877 −2493 379 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 374 (M) −584 −1354 −847 −246 −1467 −16592505 −1087 212 −374 2571 −449 −1729 1171 1074 −634 −507 −876 −1617 −1128380 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 375 (P)−910 −2031 −73 1195 −2792 −1488 −794 −2539 −629 −2588 −1788 −401 3005−439 −1131 612 −1014 −2050 −2815 −2151 381 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 376 (W) −1588 −1300 −3783 −3197 −329−3245 −1926 2071 −2827 1901 558 −2822 −3072 −2297 −2616 −2381 −1508 −1113483 −1042 382 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 377 (E) −1024 −2640 1844 2310 −2908 −1498 −505 −2711 −344−2636 −1791 −107 −1824 1521 −957 207 −1011 −2243 −2817 −2021 383 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 378 (N) −826 −23491089 227 −2651 −1487 −341 −2416 1494 −2346 −1475 2601 −1724 1005 −522−657 −787 −1968 −2511 −1791 384 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 379 (P) 1932 −1116 −2232 −2301 −3058 −1358 −2206−2706 −2336 −3009 −2238 −1674 3274 −2114 −2406 −739 −914 −1913 −3260−3019 385 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *380 (V) −914 −773 −2713 −2129 −712 −2505 −1388 1452 1084 1324 204 −1926−2507 −1580 −1808 −1591 −859 1713 −1424 −1081 386 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 381 (Y) −1484 −2331 −1762 −887−2436 −2254 −420 −2325 2137 −2195 −1475 −949 −2258 −39 1983 −1411 −1295−2075 −2087 2868 387 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 382 (E) 1256 −1890 −206 1353 −2196 −1401 −89 −1930 812 −1898−996 −45 547 1252 −162 −356 −414 −1507 −2083 −1416 388 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 383 (Q) −752 −2272 1586 1407−2561 −1448 −308 −2329 −23 −2276 −1396 −71 −1677 1749 −577 −590 1569−1881 −2459 −1727 389 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 384 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 390 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 385 (H) −964−2089 −200 −136 −2264 −1600 3833 −2320 −296 −2338 −1558 1362 1479 −276−699 −881 −992 −1924 −2364 −1652 391 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 386 (L) −2451 −1983 −4707 −4186 −582−4409 −3259 1510 −3884 2778 592 −4069 −3865 −3091 −3590 −3698 −2355 −150−2226 −2214 392 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 387 (Q) 1643 −1017 −1196 −721 −1189 −1714 −668 1336 −497 −907−297 −823 −1893 2044 −794 −784 −569 −339 −1579 −1135 393 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 388 (I) −1760 −1308 −4323−3961 −1730 −4039 −3721 3156 −3825 −575 −512 −3720 −3867 −3669 −3893−3356 −1753 2241 −3236 −2802 394 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 389 (L) −2871 −2457 −4231 −4103 −1033 −3803−3165 −541 −3734 3130 −31 −3935 −3797 −3286 −3484 −3713 −2869 −1136−2394 −2220 395 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 390 (K) −1259 −2115 −1267 −676 −970 −2105 1794 −2040 2549−1955 −1282 −808 −2165 −167 114 −1192 −1140 −1801 −1301 2517 396 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 391 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 397 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 392 (N) −2171 −2655 −1458 −1748−3334 −2364 −2267 −3943 −2365 −3936 −3437 4205 −2932 −2205 −2608 −2224−2439 −3392 −3253 −2909 398 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 393 (L) −2871 −2457 −4231 −4103 −1033 −3803 −3165−541 −3734 3130 −31 −3935 −3797 −3286 −3484 −3713 −2869 −1136 −2394−2220 399 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *394 (A) 3121 −934 −2489 −2561 −3081 −1203 −2295 −2766 −2533 −3080 −2234−1669 −1953 −2234 −2533 936 −746 −1844 −3331 −3090 400 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 395 (E) −522 −1773 −240 1676−2248 −1396 −289 −1968 50 −1989 −1115 −174 1198 131 −448 1226 677 −1538−2214 −1565 401 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 396 (E) −1481 −3230 1425 2936 −3481 751 −843 −3354 −954 −3256−2520 −187 −2057 −492 −1711 −1193 −1527 −2852 −3445 −2523 402 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 397 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 403 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 398 (A) 2847 −932 −2454 −2477−3066 −1198 −2236 −2763 −2439 −3057 −2202 −1635 −1940 −2152 −2471 1777−731 −1840 −3306 −3056 404 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 399 (V) −1771 −1603 −3750 −3689 −2037 −3050 −3231 403−3479 −1154 −1076 −3246 −3399 −3383 −3437 −2628 −1917 3536 −3074 −2677405 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 400 (A)3438 −1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662 −2236−2447 −2798 −2944 −1216 −1387 −2183 −3405 −3320 406 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 401 (K) −2620 −2961 −2461−2046 −3743 −2791 −1570 −3603 3784 −3387 −2839 −2048 −3039 −1260 −465−2604 −2536 −3331 −3001 −2988 407 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 402 (I) −1761 −1312 −4317 −3954 −1713 −4027−3703 3225 −3814 −556 −498 −3712 −3859 −3653 −3877 −3344 −1754 2110−3216 −2787 408 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 403 (S) −348 −981 −2200 −2194 −2989 −1227 −2073 −2686 −2157−2970 −2136 −1541 −1946 −1946 −2253 3060 1398 −1824 −3217 −2916 409 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 404 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 410 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 405 (V) −917 −809 −2556 −1976 −827−2491 −1367 1339 1455 721 94 −1841 −2501 −1487 −1710 −1570 −863 2038−1514 −1151 411 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 406 (K) −1386 −2643 −447 1824 −3108 −1893 −570 −2762 2860−2616 −1848 −552 −2117 −166 −3 −1217 −1300 −2388 −2647 −2154 412 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 407 (N) −537 −1563−449 −36 −1889 1143 −307 −1529 932 −1655 −844 1794 −1658 73 −356 −518−516 924 −1962 −1392 413 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 408 (P) −894 −2181 −369 1705 −2576 −1650 −357 −2268 243−2210 −1375 −330 2093 63 1619 −774 −835 −1876 −2347 −1769 414 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 409 (V) −419 −634−1376 −807 1053 −1737 −499 −198 −623 −505 178 600 −1807 −475 475 313−360 1389 −1016 1303 415 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 410 (I) −1282 −1082 −3022 −2555 2426 −2683 1767 2555−2191 −443 −88 −2038 −2692 −1794 −2075 −1793 −1220 −317 −361 552 416 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 411 (T) −499−1595 −431 966 −1830 −1487 −185 −1449 1092 −1574 −754 −207 −1601 213−206 −458 2067 159 −1877 −1296 417 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 412 (G) −2594 −2690 −3304 −3623 −4328 3747−3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004−3668 −4222 418 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 413 (P) −632 −1230 −2074 −2144 −2996 −1453 −2116 −2631 −2128−2928 −2213 −1658 3610 −2006 −2221 −852 1302 −1931 −3185 −2917 419 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 414 (A) 3438−1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028 −3257 −2662 −2236 −2447−2798 −2944 −1216 −1387 −2183 −3405 −3320 420 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 415 (R) −1454 −2316 −1780 −878−2834 −2232 −428 −2292 2281 −2200 −1473 −940 −2240 −17 2627 −1386 −1270588 −2249 −1960 421 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 416 (V) −1771 −1603 −3750 −3689 −2037 −3050 −3231 403 −3479−1154 −1076 −3246 −3399 −3383 −3437 −2628 −1917 3536 −3074 −2677 422 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 417 (F) −3342−2776 −4026 −4232 4354 −3545 −1431 −2315 −4038 −1801 −1900 −3299 −3780−3350 −3645 −3490 −3420 −2566 −739 349 423 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 418 (D) −1572 −3426 2573 2447 −3613−1583 −879 −3513 −1050 −3393 −2684 1292 −2085 −535 −1855 −1253 −1623−3000 −3585 −2609 424 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 419 (S) −879 −1989 1498 −177 −3045 1600 −939 −2843 −904 −2867−2046 −438 −1922 −591 −1483 2171 −1044 −2226 −3072 −2372 425 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 420 (E) −2641 −3308−896 3732 −3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842−2560 −2479 −2750 −3722 −3563 −3385 426 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 421 (Q) −705 −1925 −199 2112 917 −1534−288 −1824 42 −1842 −1054 −210 −1709 2163 −420 −611 −656 −1502 −1997−1291 427 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *422 (H) −569 −2048 1450 1526 −2349 −1405 1830 −2103 181 −2058 −1157 −37−1569 272 −349 713 620 −1662 −2240 −1537 428 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 423 (C) 1626 2878 −2671 −2107 1264 −1968−1091 233 −1777 −334 250 −1672 −2128 −1459 −1691 −1096 −529 1209 −1066−704 429 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *424 (M) −2042 −1634 −4379 −3826 −659 −3976 −2899 2765 −3546 1204 3085−3605 −3604 −2896 −3318 −3183 −1961 195 −2135 −2058 430 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 425 (E) 412 −2447 1356 2379−2747 −1477 −445 −2527 −243 −2477 −1622 −107 855 −36 −831 −730 −894−2073 −2668 −1906 431 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 426 (A) 2822 −1031 −2418 −2539 −3226 1898 −2364 −2941 −2626−3229 −2379 −1722 −2026 −2302 −2634 −654 −848 −1983 −3415 −3226 432 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 427 (I) −1772−1325 −4307 −3877 −1405 −3993 −3383 2935 −3705 820 −217 −3632 −3761−3400 −3682 −3260 −1742 2033 −2838 −2525 433 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 428 (L) −875 −1634 −575 959 −1581 −1769−525 −1179 −135 1884 −625 −547 −1931 1405 −450 −909 −816 −1074 −1883−1383 434 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *429 (A) 1705 −1826 −180 949 −2318 −1410 −359 −2041 −53 −2067 −1204 1001−1652 52 −561 1232 −595 −1609 −2298 −1643 435 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 430 (D) −1074 −2458 2381 60 −29211927 −658 −2710 −463 −2675 −1860 −271 −1918 −276 866 −915 −1100 −2245−2845 −2124 436 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 431 (K) −688 −2117 785 888 −2469 −1529 −187 −2189 2380 −2106−1221 −162 −1661 256 1134 −553 −619 −1760 −2240 −1607 437 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 432 (I) −2019 −1582−4380 −3941 −1000 −4086 −3253 3295 −3671 1100 145 −3736 −3783 −3222−3556 −3378 −1976 657 −2517 −2289 438 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 433 (Q) −490 −1797 −369 171 −2078 −14571762 −1779 1157 −1780 −905 1165 −1550 1798 −48 −396 −422 725 −1986 −1366439 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 434 (A)1954 −1836 1733 −180 −2714 −1429 −806 −2438 −679 −2518 −1698 −430 1775−448 −1211 −736 −894 −1923 −2765 −2117 440 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 435 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 441 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 436 (D) −1736 −3455 3490 97 −3737 −1646 −1070 −3753 −1363−3647 −3016 1602 −2204 −760 −2218 −1420 −1838 −3213 −3756 −2780 442 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 437 (V) −1721−1302 −4229 −3874 −1705 −3894 −3582 1607 −3706 −582 −513 −3610 −3786−3559 −3767 −3209 −1725 3294 −3158 −2712 443 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 438 (V) 594 −988 −3391 −2911 −1164 −2888−2187 845 −2637 765 −154 −2576 −2962 −2387 −2622 −2074 −1205 2800 −2084−1724 444 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *439 (V) −1771 −1603 −3750 −3689 −2037 −3050 −3231 403 −3479 −1154 −1076−3246 −3399 −3383 −3437 −2628 −1917 3536 −3074 −2677 445 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 440 (I) −1754 −1308 −4295−3867 −1434 −3978 −3377 2661 −3697 862 −247 −3617 −3754 −3406 −3679−3243 −1725 2373 −2852 −2526 446 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 441 (R) −2957 −3022 −3318 −2735 −3796 −2998−1968 −3912 −846 −3631 −3157 −2611 −3280 −1724 4056 −3026 −2913 −3650−3096 −3185 447 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 442 (Y) −1321 −1438 −1994 −1608 2186 527 −450 −1117 −1481−1211 −693 1178 −2522 −1217 −1665 −1518 −1275 −1021 −198 3178 448 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 443 (C) −675 2205−2544 972 −572 −2236 −1121 1373 −1671 679 261 −1700 −2270 −1403 −1668−1311 −621 1601 −1150 −790 449 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 444 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462−4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668−4222 450 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *445 (P) −2931 −2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490−4165 −3491 4225 −3781 −3695 −3182 −3279 −4087 −3594 −4064 451 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 446 (K) −1060−2058 −1088 −460 −2432 −1917 −357 −1970 2801 −1978 −1220 −632 −1990 1339367 −999 −946 536 −2145 −1717 452 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 447 (G) −2594 −2690 −3304 −3623 −4328 3747−3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004−3668 −4222 453 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 448 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 454 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 449 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 455 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 450 (G) −2594 −2690 −3304 −3623−4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839−2981 −4004 −3668 −4222 456 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 451 (M) −2406 −2296 −3638 −3594 −1525 −3105 −2824−1047 −3121 −596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398 −2513−2207 457 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *452 (P) −1659 −2241 −2022 −1646 −3185 −2242 −1373 −3000 −450 −2936 −2274−1624 3435 −1065 2095 −1730 −1750 −2593 −2816 −2613 458 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 453 (E) −2641 −3308 −8963732 −3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560−2479 −2750 −3722 −3563 −3385 459 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 454 (M) −2406 −2296 −3638 −3594 −1525 −3105−2824 −1047 −3121 −596 5043 −3293 −3425 −3046 −2996 −2911 −2552 −1398−2513 −2207 460 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 455 (L) −2871 −2457 −4231 −4103 −1033 −3803 −3165 −541 −37343130 −31 −3965 −3797 −3286 −3484 −3713 −2869 −1136 −2394 −2220 461 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 456 (K) 1368−1491 −763 −332 −2319 −1417 −551 −1998 1786 −2068 −1221 −500 −1721 −160−470 1631 −587 −1532 −2299 −1754 462 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 457 (P) −1500 −1738 −2514 −2380 −1555−2358 −2022 −1126 −2063 1224 −841 −2189 3436 −2061 −2129 −1822 −1674−1231 −2290 −1878 463 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 458 (T) −351 −974 −2208 −2185 −2894 −1237 −2041 −2561 −2125−2863 −2046 −1539 −1948 −1923 −2218 1543 3230 −1758 −3139 −2834 464 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 459 (S) −897−1462 −2333 −2543 −3185 −1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360−2483 −2703 3465 −1316 −2413 −3310 −3025 465 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 460 (M) 2706 −986 −2433 −2144 −1502−1684 −1706 −700 −1858 −968 2744 −1705 −2188 −1713 −1932 −963 −862 −592−2145 −1794 466 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 461 (I) −2103 −1659 −4461 −3992 −869 −4152 −3233 3082 −37231619 290 −3801 −3788 −3171 −3557 −3432 −2046 487 −2418 −2265 467 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 462 (I) −1761−1312 −4317 −3954 −1713 −4027 −3703 3225 −3814 −556 −498 −3712 −3859−3653 −3877 −3344 −1754 2110 −3216 −2787 468 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 463 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 469 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 464 (K) 1641 −2033 −323 914 −2415 −1565 −296 −2097 2052 −2080−1233 −257 −1736 125 −133 −646 −702 −1707 −2258 −1657 470 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 465 (G) −2594 −2690−3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748−3779 −2839 −2981 −4004 −3668 −4222 471 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 466 (L) −1699 −1807 −2268 −1925 −830−2795 −1551 −455 −1225 2510 90 −1958 −2845 1927 −1308 −2067 −1651 −846−1841 −1454 472 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 467 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 473 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 468 (D) −853−2415 2115 1717 −2702 −1468 −378 −2484 1085 −2417 −1546 −84 −1732 41−699 696 −824 −2025 −2594 −1839 474 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 469 (S) −892 −1780 −931 −688 −2757 −1643 −830−2472 1671 −2492 −1708 −799 −2018 −468 −365 2676 −1004 −1981 −2598 −2130475 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 470 (C)−1135 3503 −3700 −3406 −1670 −2549 −2675 653 −3101 −916 −667 −2727 −2925−2870 −3030 −1868 −1288 2927 −2619 −2222 476 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 471 (A) 2590 −1035 −2404 −2530 −32362290 −2365 −2954 −2627 −3240 −2389 −1719 −2027 −2302 −2637 −656 −851−1991 −3423 −3234 477 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 472 (L) −2632 −2152 −4630 −4185 1767 −4324 −2442 −61 −38792789 563 −3833 −3823 −2970 −3513 −3609 −2518 −738 −1527 −945 478 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 473 (I) −2073−1632 −4434 −3975 −911 −4130 −3238 3164 −3706 1451 244 −3779 −3785 −3187−3557 −3413 −2021 546 −2449 −2273 479 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 474 (T) −1213 −1674 −2755 −2906 −3163−1922 −2659 −2698 −2788 −3105 −2612 −2311 −2600 −2708 −2753 −1463 3819−2197 −3286 −3156 480 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 475 (D) −2784 −3432 4016 −1200 −4140 −2466 −2197 −4505 −2621−4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938 −4046 −3710 −3552 481 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 476 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 482 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 477 (R) −2957 −3022 −3318 −2735−3796 −2998 −1968 −3912 −846 −3631 −3157 −2611 −3280 −1724 4056 −3026−2913 −3650 −3096 −3185 483 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 478 (F) −3342 −2776 −4026 −4232 4354 −3545 −1431−2315 −4038 −1801 −1900 −3299 −3780 −3350 −3645 −3490 −3420 −2566 −739349 484 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 479(S) −897 −1462 −2333 −2543 −3185 −1640 −2474 −3294 −2686 −3497 −2780−1973 −2360 −2483 −2703 3465 −1316 −2413 −3310 −3025 485 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 480 (G) −2594 −2690 −3304−3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779−2839 −2981 −4004 −3668 −4222 486 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 481 (G) −2594 −2690 −3304 −3623 −4328 3747−3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004−3668 −4222 487 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 482 (T) −359 −976 −2225 −2229 −2900 −1242 −2074 −2560 −2170−2875 −2064 −1561 −1958 −1969 −2247 1110 3375 −1760 −3152 −2850 488 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 483 (Y) −3402−2632 −3941 −4011 1064 −3924 3388 −2526 −3541 −1996 −1973 −2625 −3821−2664 −3170 −3135 −3280 −2619 3420 3756 489 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 484 (G) −2594 −2690 −3304 −3623 −43283747 −3462 −4761 −3953 −4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981−4004 −3668 −4222 490 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 485 (M) −2322 −1904 −4536 −3951 2387 −4112 −2676 67 −3649 20343156 −3710 −3633 −2803 −3311 −3309 −2204 −588 −1794 −1586 491 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 486 (V) −1771−1603 −3750 −3689 −2037 −3050 −3231 403 −3479 −1154 −1076 −3246 −3399−3383 −3437 −2628 −1917 3536 −3074 −2677 492 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 487 (V) −1771 −1603 −3750 −3689 −2037−3050 −3231 403 −3479 −1154 −1076 −3246 −3399 −3383 −3437 −2628 −19173536 −3074 −2677 493 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 488 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 494 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 489 (H) −3205−3079 −2723 −2890 −2110 −3046 5295 −4135 −2617 −3813 −3561 −2886 −3482−2833 −2620 −3291 −3356 −3895 −2397 −1681 495 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 490 (V) −1754 −1297 −4329 −3968−1770 −4053 −3752 2604 −3840 −621 −545 −3728 −3878 −3699 −3917 −3370−1746 2859 −3276 −2829 496 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 491 (A) 2587 −828 −2477 −2155 −1837 −1468 −1728 −743−1941 −1564 −954 −1607 −2033 −1725 −2034 −738 1178 1108 −2310 −1972 497— −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 492 (P) −2931−2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −3491 4225−3781 −3695 −3182 −3279 −4087 −3594 −4064 498 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 493 (E) −2641 −3308 −896 3732−3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479−2750 −3722 −3563 −3385 499 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 494 (A) 3438 −1472 −2846 −3040 −3287 −1726 −2735−2840 −3028 −3257 −2662 −2236 −2447 −2798 −2944 −1216 −1387 −2183 −3405−3320 500 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *495 (Y) −866 −976 −1863 −1331 1353 −2145 1318 −556 −1116 −777 −173 −1242−2197 1714 −1301 −1173 −802 888 −445 2749 501 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 496 (D) 417 −1831 1647 1094 −2065−1488 −353 −1618 −107 −1820 −1019 −189 −1698 30 −623 −603 −643 1629−2154 −1520 502 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 497 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 503 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 498 (G) −2594−2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320 −3352−3748 −3779 −2839 −2981 −4004 −3668 −4222 504 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 499 (T) 492 −1190 −706 −181 −1475311 −333 −1099 −81 71 −509 570 1113 −6 −509 −450 1123 −835 −1680 −1161505 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 500 (I)−2091 −1746 −3971 −3840 −1676 −3532 −3289 3684 −3581 −659 −693 −3562−3674 −3445 −3521 −3194 −2146 449 −2877 −2493 506 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 501 (A) 3103 −1036 −2445 −2572−3222 1051 −2380 −2930 −2650 −3226 −2381 −1739 −2034 −2327 −2648 −664−857 −1981 −3412 −3228 507 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 502 (L) −2239 −1892 −3711 −3400 301 −3520 −1210 −542−2948 2564 −35 −2786 −3395 −2438 −2750 −2747 −2165 −945 −573 2562 508 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 503 (V) −1757−1387 −4101 −3681 −1174 −3714 −3031 880 −3410 1254 −60 −3407 −3585 −3094−3354 −2984 −1743 3014 −2536 −2219 509 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 504 (Q) −982 −2251 −866 971 −2711 −1822−252 −2340 1444 −2194 −1356 −464 −1885 2646 1632 −858 −863 −1958 −2245−1765 510 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *505 (E) −1162 −2771 2137 2239 −3046 −1526 −626 −2849 −546 −2792 −1983−145 −1905 −242 −1192 −940 1396 −2385 −2990 −2169 511 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 506 (G) −1707 −2684 1591 −614−3783 3190 −1613 −3795 −1887 −3775 −3119 −915 −2456 −1358 −2539 −1610−1924 −3150 −3636 −3124 512 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 507 (D) −2784 −3432 4016 −1200 −4140 −2466 −2197−4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938 −4046 −3710−3552 513 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *508 (M) −473 −522 −1819 −1236 −468 −1879 −687 1519 −996 566 1677 −1154−1937 836 −1131 1079 −413 102 −957 −585 514 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 509 (I) −1761 −1312 −4317 −3954 −1713−4027 −3703 3225 −3814 −556 −498 −3712 −3859 −3653 −3877 −3344 −17542110 −3216 −2787 515 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 510 (T) 782 −1467 −550 1029 −2202 −1425 −709 −1791 −472 −1993−1203 −528 −1787 −368 −902 −617 2685 −1400 −2333 −1783 516 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 511 (I) −1766 −1333−4283 −3923 −1635 −3967 −3619 3388 −3759 −473 −437 −3672 −3822 −3576−3804 −3285 −1764 1695 −3126 −2717 517 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 512 (D) −2784 −3432 4016 −1200 −4140−2466 −2197 −4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593 −2938−4046 −3710 −3552 518 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 513 (A) 2705 −1451 −1036 −913 −2506 −1504 −1143 −2174 −794−2337 −1613 −946 −1993 2040 −1061 −809 −910 −1703 −2633 −2156 519 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 514 (H) −615 −16801444 66 −1883 168 2650 −1558 −86 −1691 −891 −223 −1680 31 −577 −571 −5851267 −2007 −1397 520 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 515 (K) −654 −2006 −546 42 −2376 −1581 −133 −2066 1935 −1987−1107 1132 −1658 1043 1058 −540 1180 −1660 −2113 −1532 521 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 516 (N) −933 −2085 −946−284 −2472 −1822 −253 −2090 1711 76 −1204 1918 −1876 175 1799 −841 −817−1755 −2132 −1663 522 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 517 (E) −416 −987 −843 1107 −1070 −1583 −338 −623 −183 879−172 −489 −1679 −94 −565 544 813 265 −1379 −905 523 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 518 (I) −2258 −1804 −4588−4084 −706 −4269 −3231 2527 −3807 2292 465 −3923 −3814 −3118 −3570 −3544−2181 190 −2303 −2237 524 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 519 (Q) −477 −1909 958 282 −2211 −1389 1484 −1953 285−1921 −1018 −32 −1517 2318 −225 630 559 −1525 −2110 −1430 525 — −149−500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369−294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 520 (L) −2127−1743 −4402 −3796 1257 −3918 −2674 149 −3492 2527 2164 −3553 −3509 −2714−3181 −3095 −2019 570 −1870 −1818 526 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 521 (N) −723 −2217 958 236 −2518 −14661611 −2279 1719 −2217 −1334 2285 −1666 166 −401 −570 −677 −1837 −2382−1678 527 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *522 (V) −1754 −1297 −4330 −3968 −1770 −4053 −3752 2623 −3841 −620 −545−3729 −3878 −3699 −3918 −3371 −1746 2846 −3277 −2830 528 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 523 (S) 1545 −974 −2003−1825 −2867 −1206 −1790 −2580 −1788 −2795 −1932 −1362 1826 −1586 −19992362 −672 −1755 −3057 −2721 529 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 524 (D) −1776 −3649 3326 1869 −3838 −1642 −1031−3788 −1322 −3660 −3029 −245 −2192 −711 −2201 −1425 −1855 −3264 −3821−2816 530 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *525 (E) 423 −2950 1944 2696 −3223 −1545 −718 −3047 −715 −2979 −2196 −161−1968 −347 −1403 −1043 −1314 −2569 −3177 −2316 531 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 526 (E) −2641 −3308 −896 3732−3966 −2458 −2043 −4105 −2128 −4016 −3555 −1531 −2959 −1842 −2560 −2479−2750 −3722 −3563 −3385 532 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894−1115 −701 −1378 * * 527 (L) −2339 −1899 −4618 −4042 1570 −4204 −28491440 −3758 2558 676 −3825 −3700 −2902 −3418 −3418 −2226 −382 −1924 −1778533 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 528 (A)2338 −1990 −241 938 −2395 −1557 −423 −2061 954 −2103 −1286 −301 −1791−26 −375 −717 −784 −1691 −2330 −1728 534 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 529 (R) 524 −2098 −789 −146 −2504 −17291632 −2153 1229 −2054 −1204 −379 −1789 1328 2313 −719 −724 −1774 −2150−1637 535 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *530 (R) −2957 −3022 −3318 −2735 −3796 −2998 −1968 −3912 −846 −3631 −3157−2611 −3280 −1724 4056 −3026 −2913 −3650 −3096 −3185 536 — −149 −500 23343 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249— −16 −7108 −8150 −894 −1115 −701 −1378 * * 531 (R) −1895 −2713 −2327−1192 −3484 −2502 −481 −2856 2144 −2544 −1842 −1161 −2458 1393 3023−1770 −1619 −2599 −2421 −2259 537 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 532 (A) 2935 −1714 −553 857 −2769 −1546 −1218−2333 −1106 −2591 −1873 −809 −2065 −934 −1502 −954 −1103 −1872 −2898−2374 538 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *533 (A) 1291 −1874 −176 1227 −2177 −1392 −109 −1909 277 −1891 −995 1134−1522 1248 −228 −361 562 −1492 −2090 −1419 539 — −149 −500 233 43 −381399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16−7108 −8150 −894 −1115 −701 −1378 * * 534 (W) −805 −687 −2581 −2028 138−2236 −697 897 −1681 −421 141 −1645 −2282 −1369 −1627 −1315 636 −90 44791809 540 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 4596 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * *535 (H) −408 −1801 −274 1284 −2096 −1385 1500 −1822 1168 −1802 −899 −33−1479 1381 −102 −303 595 221 −1996 −1339 541 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 536 (Q) −650 −1737 −627 −72 −1981 −1615−209 −1625 1223 392 −866 −318 1222 2120 50 −598 −572 −1326 −1932 −1394542 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 537 (P)−2931 −2878 −3420 −3706 −4181 −2925 −3468 −4621 −3859 −4490 −4165 −34914225 −3781 −3695 −3182 −3279 −4087 −3594 −4064 543 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−324 −7108 −2368 −894 −1115 −701 −1378 * * 538 (A) 2195 −924 −968 −546−1397 −1356 −583 −812 −365 −1167 −487 −618 −1660 1324 −684 −483 −404 462−1703 −1242 544 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −19 −6804 −7846 −894 −1115 −428−1961 * * 539 (P) 411 −1017 −1886 −1616 −1600 −1588 −1411 −962 −1408 495−755 −1384 3156 −1323 −1577 −847 −785 −783 −2111 −1716 545 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 540 (R) −1612 −2397−2037 −1033 −2897 −2352 −458 −2365 2184 665 −1520 −1051 −2334 −51 2602−1545 −1395 −2143 −2262 −2014 546 — −149 −500 233 43 −381 399 106 −626210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150−894 −1115 −701 −1378 * * 541 (Y) 712 −796 −2334 −1883 −370 −2028 −986−143 −1607 −663 −131 −1587 −2243 −1383 −1656 −1178 −771 1114 −965 3479547 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 542 (T)−527 −1669 1091 −27 −2315 −1379 −443 −2033 −151 −2081 −1218 −282 557 −41−650 1128 2077 −1576 −2321 −1690 548 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 543 (R) −2957 −3022 −3318 −2735 −3796−2998 −1968 −3912 −846 −3631 −3157 −2611 −3280 −1724 4056 −3026 −2913−3650 −3096 −3185 549 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 544 (G) −2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953−4671 −4212 −3320 −3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 550 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 545 (V) −1747−1296 −4310 −3948 −1758 −4023 −3716 2215 −3813 −615 −540 −3705 −3860−3670 −3887 −3339 −1741 3087 −3252 −2806 551 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 546 (L) −2871 −2457 −4231 −4103 −1033−3803 −3165 −541 −3734 3130 −31 −3935 −3797 −3286 −3484 −3713 −2869−1136 −2394 −2220 552 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 547 (A) 2404 −890 −1926 −1629 −1803 1275 −1415 −1282 −1490 392−963 −1316 −1930 −1328 −1674 −654 −644 −952 −2187 −1810 553 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 548 (K) −2620 −2961−2461 −2046 −3743 −2791 −1570 −3603 3784 −3387 −2839 −2048 −3039 −1260−465 −2604 −2536 −3331 −3001 −2988 554 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 549 (Y) −3621 −2707 −4176 −4424 2950−4049 −394 −2539 −4002 −1942 −1987 −2749 −3933 −2854 −3451 −3299 −3499−2690 349 4094 555 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 550 (A) 3438 −1472 −2846 −3040 −3287 −1726 −2735 −2840 −3028−3257 −2662 −2236 −2447 −2798 −2944 −1216 −1387 −2183 −3405 −3320 556 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 551 (H) −1741−2627 −2070 −1046 −3303 −2401 2713 −2751 2478 −2476 −1755 −1061 −2375−27 2379 −1621 −1497 −2477 −2379 −2161 557 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 552 (L) −1014 −876 −2956 −2408 −582−2550 −1529 1721 −2079 2042 345 −2114 −2581 −1775 −2028 454 −980 286−1414 −1096 558 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 553 (V) 933 −842 −2818 −2467 −1542 −1870 −1890 154 −2226 −1095−617 −1932 −2326 −1995 −2259 −1126 1070 2769 −2180 −1826 559 — −149 −500233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294−249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 554 (S) −787 −1522−1486 −1172 −2714 −1599 −1112 −2500 −433 −2563 −1791 −1110 −2067 −7961351 2916 −989 −1943 −2648 −2234 560 — −149 −500 233 43 −381 399 106−626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 555 (S) −326 −1010 −1779 −1541 −2691−1234 −1566 −2386 −1486 −2594 −1749 −1228 1196 −1330 −1747 2396 1967−1662 −2876 −2496 561 — −149 −500 233 43 −381 399 106 −626 210 −466 −720275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701−1378 * * 556 (A) 3121 −934 −2489 −2561 −3081 −1203 −2295 −2766 −2533−3080 −2234 −1669 −1953 −2234 −2533 936 −746 −1844 −3331 −3090 562 —−149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359 117−369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 557 (S) −897−1462 −2333 −2543 −3185 −1640 −2474 −3294 −2686 −3497 −2780 −1973 −2360−2483 −2703 3465 −1316 −2413 −3310 −3025 563 — −149 −500 233 43 −381 399106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108−8150 −894 −1115 −701 −1378 * * 558 (R) −586 −1873 −516 979 −2188 −1543−123 −1869 1290 −353 −980 −202 −1622 314 1886 −491 782 −1495 −2024 −1439564 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 559 (G)−2594 −2690 −3304 −3623 −4328 3747 −3462 −4761 −3953 −4671 −4212 −3320−3352 −3748 −3779 −2839 −2981 −4004 −3668 −4222 565 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 560 (C) 2804 3772 −3185 −3198−2739 −1303 −2462 −2065 −2882 −2628 −1924 −1927 −2044 −2547 −2727 −661−799 −1463 −3099 −2886 566 — −149 −500 233 43 −381 399 106 −626 210 −466−720 275 394 45 96 359 117 −369 −294 −249 — −16 −7108 −8150 −894 −1115−701 −1378 * * 561 (V) −1771 −1603 −3750 −3689 −2037 −3050 −3231 403−3479 −1154 −1076 −3246 −3399 −3383 −3437 −2628 −1917 3536 −3074 −2677567 — −149 −500 233 43 −381 399 106 −626 210 −466 −720 275 394 45 96 359117 −369 −294 −249 — −16 −7108 −8150 −894 −1115 −701 −1378 * * 562 (T)−1213 −1674 −2755 −2906 −3163 −1922 −2659 −2698 −2788 −3105 −2612 −2311−2600 −2708 −2753 −1463 3819 −2197 −3286 −3156 568 — −149 −500 233 43−381 399 106 −626 210 −466 −720 275 394 45 96 359 117 −369 −294 −249 —−16 −7108 −8150 −894 −1115 −701 −1378 * * 563 (D) −2784 −3432 4016 −1200−4140 −2466 −2197 −4505 −2621 −4365 −3956 −1551 −3014 −2039 −3232 −2593−2938 −4046 −3710 −3552 569 — −149 −500 233 43 −381 399 106 −626 210−466 −720 275 394 45 96 359 117 −369 −294 −249 — −21 −6715 −7757 −894−1115 −701 −1378 * * 564 (F) −525 −445 −2202 −1627 1946 −2001 −744 1247−1346 952 561 1079 −2030 −1067 −1362 −1067 −465 338 −714 −230 570— * * * * * * * * * * * * * * * * * * * * — * * * * * * * * 0 HMMER2.0[2.2g] Program name and version NAME dhad_for_hmm Name of the inputsequence alignment file LENG 564 Length of the alignment: include indelsALPH Amino Type of residues MAP yes Map of the match states to thecolumns of the alignment COM/app/public/hmmer/current/bin/hmmbuild -Fdhad-exp_hmm dhad_for_hmm.aln Commands used to generate the file: thisone means that hmmbuild (default patrameters) was applied to thealignment file COM/app/public/hmmer/current/bin/hmmcalibratedhad-exp_hmm Commands used to generate the file: this one means thathmmcalibrate (default parametrs) was applied to the hmm profile NSEQ 8Number of sequences in the alignment file DATE Tue Jun 3 10:48:24 2008When was the file generated XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4NULT −4 −8455 The transition probability distribution for the null model(single G state). NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085−142 −21 −313 45 531 201 384 −199 The symbol emission probabilitydistribution for the null model (G state); consists of K (e.g. 4 or 20)integers. The null probability used to convert these back to modelprobabilities is 1/K. EVD −499.650970 0.086142 The extreme valuedistribution parameters μ and lambda respectively; both floating pointvalues. Lambda is positive and nonzero. These values are set when themodel is calibrated with hmmcalibrate.

What is claimed is:
 1. A recombinant yeast host cell comprising at leasttwo engineered modifications comprising: (i) a heterologousdihydroxy-acid dehydratase Fe—S cluster protein expressed in the cytosolof the recombinant yeast host cell, wherein the heterologousdihydroxy-acid dehydratase Fe—S cluster protein is a polypeptide havingan amino acid sequence that is at least 90% identical to SEQ ID NO: 114using the Clustal W method of alimment using the default parameters ofGAP PENALTY=10, GAP LENGTH PENALTY=0,1, and Gonnet 250 series of proteinweight matrix over the full length of the protein sequence and has aspecific activity of at least 0.2 μmol min⁻¹ mg⁻¹; and (ii) anendogenous Fe—S cluster protein with reduced expression, wherein theendogenous Fe—S cluster protein is dihydroxy-acid dehydratase.
 2. Therecombinant yeast host cell of claim 1, wherein the yeast is selectedfrom the group consisting of Saccharomyces, Schizosaccharomyces,Hansenula, Candida, Kluyveromyces, Yarrowia and Pichia.
 3. Therecombinant yeast host cell of claim 1, wherein the endogenous Fe—Scluster protein is expressed in the mitochondria.
 4. The recombinantyeast host cell of claim 1, wherein the host cell is Saccharomyces thatexpresses a gene encoding a polypeptide having the amino acid sequenceas set forth in SEQ ID NO:114.
 5. The recombinant yeast host cell ofclaim 1, wherein the cell comprises an isobutanol biosynthetic pathway.6. The recombinant yeast host cell of claim 5, wherein the cell producesisobutanol.
 7. A method for the production of isobutanol comprisinggrowing the recombinant yeast host cell of claim 6, under conditionswherein isobutanol is produced.
 8. A method for the conversion of2,3-dihydroxyisovalerate to α-ketoisovalerate comprising: a) providing(1) the recombinant yeast host cell of claim 1; and (2) a source of2,3-dihydroxyisovalerate; and b) growing the recombinant host cell of a)with said source of 2,3-dihydroxyisovalerate under conditions where the2,3-dihydroxyisovalerate is converted by the host cell toα-ketoisovalerate.